Finding and Isolating Phages 

Yes, this was modified at the link above. Thank you for your suggestions.

Thank you for very nice video demonstration. I would appreciate to receive information that noutinf.michodigni7943 has asked .

Sorry to confuse. Great comment! That last plate was the result of the plaque isolated and replated to make a plate lysate. That is why the plaques were homogeneous. They were all derived from a single plaque! The plate is then used for further propagation and purification of the phage. Usually we get a lot of different looking phage plaques. But because you use only one isolation strain you are going to be biased.

@@BCMTAILORLabs aha! So your overnight culture of bacteria was MRSA, and at the end you show the endresult of all your work? I assumed it was E. coli since you mentioned that species in the intro, but now I understand that was just an anecdote. I like the sound of that, a lot of different looking plaques, so you are peeking into a microbial jungle! Exciting.

Isn't anthrax a virus and phages are bacteria killing viruses not virus killing viruses, right?

Yes. So how this essentially works is, as we know Bacillus anthracis is a bacterium that causes anthrax disease and phages are viruses that kill bacteria. Therefore phage therapy is used to introduce bacteriophages in the host (human with anthrax) to attach & lyse with B.anthracis' cell wall, they penetrate and hijacks the entire biological machinery of the bacteria. The phage then enters either undergoes the lytic or lysogenic cycle. The lytic cycle involves the reproduction of more bacteriophages after successfully infecting the host cell to manufacture more viruses; the viruses then burst out of the cell & the parent cell disintegrates (the OG B.anthrax bacteria that was hijacked. So instead of more B.anthracis being reproduced from one cell, you have mini phages coming out. Bacteriophages are inturn harmeless to humans. In the lysogenic cycle, the phage incorporates its viral genome (DNA) into the host cell (anthrax's chromosome) genome, infecting it from within. This allows the phage DNA (prophage) to be copied and passed on along with the cell's own DNA. Hence making anthrax say goodbye to its ability to produce more copies of its-own-self. Also, apart from genetically engineering phages against B.anthracis, Phages active against B. anthracis (both lytic and lysogenic) are widespread in the environment and have been isolated from soil, carcasses, feces, sewage, and the intestinal tract of the earthworm.

Hi what's your location?

@@Fidelanne Hungary, in Europa.

@@monikafelegyhazi529 Okay please.

You are a bit out of reach logistically

@@Fidelanne Do you have phages ? Do you use phage to treat people ?

It is the same thing for me

Phage therapy is used in europe

Will fix

The link is through google docs. It seems to be working. We can send you a protocol if necessary. If you are on twitter @bcmtailorlabs direct message us.

This is different with human cells. Our expertise is only bacterial viruses.

@@BCMTAILORLabs Couldn't you just take an infected sample and find the abundant viruses that should be there? Just curious.

Î' am hope you give me a complet and easy protocle to isolat this phage.....

The buffer is here bit.ly/397acAi

The buffer is in the recipe. Detailed protocol link bit.ly/397acAi

I believe it is due to the stools and other large particles (even bacteria) blocking the filter pores as centrifuging doesn’t always “pull” all of them down.

We were referring to the difference of the clearing of a bacterial lawn verses an artifact caused by an air bubble. The best way to know is to just pick anything that looks like a plaque and replate it to make another lysate.