It's so cool that I just discovered your channel, while I'm studying for my biochemical analytics exam, and all your new videos are about my exam topics :)
Excellent video! I have a question regarding the techniques prior to SEC. Would it also be compatible to develop an HPLC-TOF-MS method to characterize an antibody and then purify it using SEC?
Yes, at least to me that sounds like a good idea! The mass spectrometry will give you the relative mass values which I believe you can use to determine the "pore size" of the beads of the size exclusion chromatography. However, I might be wrong as I'm not super familiar with the more practical aspects of size exclusion chromatography.
Interesting question. I must admit I don't know for certain but I'm sure as with almost all things in life that it's not a 100% perfect system. So yes, I do believe that happens sometimes.
