General Method of Extraction of Glycoside | Extraction of Glycoside | Glyocsides Extraction 

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GLYOSIDES
Number of medicinal plants containing organic constituents in conjugation with a sugar moiety .It can be 1or 2 .such compounds are called as glycosides. They exert therapeutically significant effect on human and animals .Traditionally used in modern medicine because of their cardio tonic, purgative, analgesic, anti-arrhythmic, demulcent action.
Definition
Glycosides are define as organic compound from plants and animal source, which on enzymatic hydrolysis gives one or more sugar moieties along with anon sugar moiety. Sugar moiety is called glycon and non sugar moiety is called aglycon or genin.
General method of Extraction
1. The method by which glycoside are isolated is called stas-otto method. The drug containing glycoside is finely powdered and subjected to successive extraction in a soxhlet apparatus with alcohol or suitable solvent.
2. During this method at first take drug containing glycoside, finely powdered that, and it is extracted with alcohol or water by using soxhlet apparatus.
3. After extraction, collect the extract and treat with lead acetate to precipitate tannins after that filter it and to the filtrate pass H2S gas, no lead acetate the precipitate as lead sulphide as this is toxic. Now after the extract again filter.
4. The filtrate is subjected to fractional crystallization, distillation or chromatography gives pure component and the extract molecular structure is determined by the spectrophotometer, Ultra Red assays, Infra red , NMR and mass spectroscopy etc
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