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GRAIN SPAWN 101, Start to Finish Tutorial on How to Make Mushroom Grain Spawn from Liquid Culture 

Renegade Mushrooms
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A start to finish tutorial on how to make mushroom grain spawn from liquid culture geared towards beginners. Learn how to turn a liquid culture syringe into pounds of grain spawn. From there you can inoculate any number of substrates with your colonized grain to grow your very own mushrooms. In this video, I cover every step of the grain spawn preparation process, explaining what I do, as well as why I do it. Hit me up in comments with any questions 🍄
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20 фев 2022

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Комментарии : 527   
@DDawg1144
@DDawg1144 6 месяцев назад
Awesome video one of the best I’ve watched, appreciate your experience.
@johnsongibbs6567
@johnsongibbs6567 9 месяцев назад
I appreciate the delicate care with which he handled that rocker.. I was worried.. but when it came time, he handled it like a pro.
@bluehoney210
@bluehoney210 Год назад
Your technique is absolutely perfect. Beautiful demonstration, thank you.
@GnarGreen
@GnarGreen Год назад
Brotha mush love for the channel. Your content is great. Helping so much on my journey. These creatures are for real. Never felt more amazing
@RenegadeMushrooms
@RenegadeMushrooms Год назад
You're hooked, I can tell 😁 Thanks for watching and the kind words.
@citylotgardening6171
@citylotgardening6171 Год назад
Your videos are very informative and easy to understand thanks for sharing 👍
@mikeschatz9153
@mikeschatz9153 11 месяцев назад
I had no idea. First time seeing anything like this. Can’t wait to see all the videos. This looks amazing. Thank you for introducing me to my new obsession.
@RenegadeMushrooms
@RenegadeMushrooms 11 месяцев назад
The rabbit hole is deep 😁
@martink9785
@martink9785 2 года назад
Great guide, thank you 👍 some day soon I'll gather the kit I need to start growing 🙂
@Schmutz-Gaming
@Schmutz-Gaming Год назад
Super educational Thanks for making this video!
@pds002
@pds002 5 месяцев назад
This is great information and not too complicated on the ingredients. After watching, I'm very enthusiastic and confident to give this a try. You are a motivating teacher. I need to watch the next stage and will look through your videos to find it. Thank you so much for sharing.
@RenegadeMushrooms
@RenegadeMushrooms 5 месяцев назад
Thank you for watching and for the kind words. I'm always here if you have questions.
@tcon6504
@tcon6504 11 месяцев назад
I’m brand new to mushroom growing. Inoculated my first grow bags July 31. Thank you for the information. I’m trying to learn as much as I can.
@RenegadeMushrooms
@RenegadeMushrooms 11 месяцев назад
Good luck on your first grow!
@LynchpinDigital
@LynchpinDigital Год назад
Clearly a pro. Excellent technique here, kids. Thanks for sharing with the world.
@RenegadeMushrooms
@RenegadeMushrooms Год назад
Thank you, I appreciate your kind words.
@ep5293
@ep5293 Год назад
You have awesome content. Thanks so much for putting this all together. I'm slowly gathering all of my supplies, and your videos really help build confidence going into this hobby. Thanks again.
@jeffreyalbright3018
@jeffreyalbright3018 11 месяцев назад
I hope you' caught some good fish when you went you took a break for the grain to chill! Thank you for the education my friend, mush love from the FLA.,🌴🏖️🍄🔥🌅
@RenegadeMushrooms
@RenegadeMushrooms 9 месяцев назад
@@jeffreyalbright3018 I did catch quite a few yellow perch that day 😁
@kingsopa4910
@kingsopa4910 9 месяцев назад
thanks a lot for the knowledge. you shared here. im starting to replicate my own spore prints from 2 years ago. so far i have been having 50/50 (only made 2 grain spawns) i hope to get better and better. will check all your channel searching for more info. have a good trip
@RenegadeMushrooms
@RenegadeMushrooms 9 месяцев назад
Cool, I recently started working with the spore to agar process myself and I'm enjoying it. Thanks for watching 👍
@serenity6988
@serenity6988 2 года назад
I would like to complain about your videos being off the charts!! Your awesome sir! And it took me less than 10 min to leave this. lol!
@RenegadeMushrooms
@RenegadeMushrooms 2 года назад
Thank you very much, that means a lot to me. Thank you for watching 👍
@markmac9515
@markmac9515 9 месяцев назад
Nice video, very clear thanks.
@CarolynsLoveRickshaw
@CarolynsLoveRickshaw Год назад
I love your content!! Thank you 🙏🏽
@CarolynsLoveRickshaw
@CarolynsLoveRickshaw Год назад
How long can fully colonized jars be stored?
@bounceurabdelaziz4973
@bounceurabdelaziz4973 Год назад
Thanks you very much for your sharing greetings to you from Algeria 🇩🇿.
@kartalbaba981
@kartalbaba981 2 года назад
Thanks for that great video!
@marlamays2131
@marlamays2131 Год назад
I've got to get some of those grains. I never thought about Chicken Chow. TY
@jasoncasey5ify
@jasoncasey5ify Год назад
This is hardest part thanks again
@dylski4294
@dylski4294 2 года назад
Man I love your videos. When I flip the tv on the first thing I check is to see if you have a new video lol. Anyway, would you ever consider doing a video on grain spawn to substrate? Using maybe a 6-20qt tub? I am a beginner. The PF Tek seems to be a little weak sauce in my opinion as far as growing potential. Learning how to grow in a tub would be ideal. Anyway, thanks again for the great content brother. You are a good dude
@RenegadeMushrooms
@RenegadeMushrooms 2 года назад
I don't do much sub tub stuff anymore other than with the mycelium flood method I use for Cordyceps. Maybe I could try to do some agaricus blazei on compost/manure at some point. I'll keep it in mind. Thanks for watching 😁
@MAGT
@MAGT Год назад
Good info bro. Thanks.
@okiedokiecookie
@okiedokiecookie Год назад
Thank you for sharing. Got my first attempt too wet. Slows down growth and very susceptible to rot. I'll do your process next attempt.
@ecoagricol65
@ecoagricol65 3 месяца назад
Nice video.Thank you.
@allenrogers6557
@allenrogers6557 Год назад
Good video. Thanks
@Unclemushlovin
@Unclemushlovin Год назад
Cheers just started my channel out. getting some good info mush lovin
@LordGeoff56
@LordGeoff56 Год назад
I'm digging how thorough you are. I'm just starting out and currently at my parents so I don't have room to set up a flow hood. I'll definitely check out more of you vids
@RenegadeMushrooms
@RenegadeMushrooms Год назад
You can do a lot without a flow hood. Focus on using liquid culture to make grain spawn and growing on pasteurized fuel pellets.
@Enochulate88
@Enochulate88 Год назад
I got a 3d printed one off Etsy for 110$
@spewter
@spewter Год назад
Instead of laminar flow, use a HEPA air purifier with a dry cleaning bag on the output. Cut two arm holes and it’ll create a clean environment for basic tasks, like inoculating spawn jars
@MrJackson66
@MrJackson66 8 месяцев назад
A cheap homemade still air box will get you growing quick, easy, and inexpensive... I have both, but usually just use my sab...
@wheremylifego3790
@wheremylifego3790 5 месяцев назад
​@RenegadeMushrooms eze bro about to try my first grow just trying to learn as much as possible first....do I need to use LC for spawning grain? Will spores still work the same? ❤
@mr.picklepants1246
@mr.picklepants1246 2 года назад
I love your channel
@mitchellflannery7013
@mitchellflannery7013 Год назад
Great video, learning a lot from u, starting to inoculate my second rye grain, first 1 was a fail. I feel more confident now thanks for sharing 👍
@RenegadeMushrooms
@RenegadeMushrooms Год назад
I'm here if you have questions 👍
@dylski4294
@dylski4294 2 года назад
Yet again another flawless video. Thanks brother. If I wanted to make only one quart jar of grain spawn, how much water would I add to the mixture grain? I know you said they expand about 40%
@RenegadeMushrooms
@RenegadeMushrooms 2 года назад
I've never done the TEK where you just add a pre-measured amount of water to the grain and PC. I always just soak in hot water overnight. You could just fill the jar a little over half full with grain, then fill the jar with hot water and soak overnight. The key is to make sure you allow the grain to air dry before packing it into the jar for the PC run. Wet grain is no bueno.
@blenderbenderguy
@blenderbenderguy 2 года назад
Thanks for another! Although I've always inoculated down the sides using the same circular motion that you use, I often wonder why not just straight down the center. Then when it's obviously colonized to the outside you're certain it's fully colonized as opposed to guessing or waiting several additional days to further be certain that it's colonized to the inside where you can't really see. Never seen that explained elsewhere, but that's the way everyone seems to do it.
@RenegadeMushrooms
@RenegadeMushrooms 2 года назад
Yeah I here ya. It's just habit for me with the LC swirlie. I guess my thought is to hit as many individual kernels with the LC as possible. But, I always give them a shake after injecting anyway, so I'm not sure it really matters.
@michaelgrouse7850
@michaelgrouse7850 2 года назад
I think the assumption is that if you squirt it down the side of the jar the liquid will fall further down between the glass and the grain and cover more area. Also you get to see if and when the mycelium take off and when its time to shake.
@dantosinferne
@dantosinferne Год назад
I put an agar wedge right in the center. They colonized fine, but I realized the main reason people say to do it near the glass is so that you can keep an eye on their progress. I think lol When it's smack dab in the center, you basically can't tell how well it's colonizing until it's almost done. so if growth slowed for some reason, you don't realize it, and can't potentially remedy (maybe you need better incubation temps, maybe you need more holes in your lids, etc). it probably matters more to newbies, able to see results faster and able to detect potential problems with their process. come to think of it, i wonder if doing a line straight through the center, as if cutting the jar in half with the lc, might be the best of both worlds. at least when it comes to jars you aren't going to shake like brf.
@mrglasecki
@mrglasecki 4 месяца назад
Outstanding
@xS.B.FEINx269
@xS.B.FEINx269 Год назад
You had me at ice fishing and michigan, go blue!!
@seanwelch1844
@seanwelch1844 9 месяцев назад
Just inoculated my first 2 bags im in Massachusetts i see the hat lol. Hoping it goes well thabks for the vid.
@RenegadeMushrooms
@RenegadeMushrooms 9 месяцев назад
Hope your first grain spawn project goes well, thx for watching 👍
@faridadoumari1927
@faridadoumari1927 2 года назад
Thank you so much
@oscardziki4543
@oscardziki4543 4 месяца назад
Thanks man. Just thanks. Awesome.
@metlwrker4285
@metlwrker4285 Год назад
On one of your vids I observed you wrapping your plates but not with parafilm. I'm very interested in how you got the plastic wrap into the 1" size. I'd love to see a vid on the process you take making the small rolls for wrapping plates and jars. Keep on teaching I'm loving the learning process!
@RenegadeMushrooms
@RenegadeMushrooms Год назад
I just cut up a roll of saran wrap with a hacksaw. I must say though, I just bought some parafilm and it works much better.
@ethankaufman8577
@ethankaufman8577 9 месяцев назад
Look for tree grafting parafilm it is much cheaper than the lab grade stuff and is usually an inch wide and much cheaper
@chepindichepindi3328
@chepindichepindi3328 11 месяцев назад
Thank you very muck.
@MrsMystic22
@MrsMystic22 11 месяцев назад
Thank you for making this video! I appreciate the time you took to explain everything the way you did. Did you simmer or boil the water separately?
@RenegadeMushrooms
@RenegadeMushrooms 11 месяцев назад
No, for this method I simply use hot tap water. No simmer or boil needed.
@MrsMystic22
@MrsMystic22 11 месяцев назад
@@RenegadeMushrooms Thank you so much for answering me!
@daveeatmon4718
@daveeatmon4718 11 месяцев назад
Awesome video, you are one of my favorite RU-vidrs to watch for sure! Now to the question. When you add this grain spawn to your pasteurized fuel pellets, what ratio do you use?
@RenegadeMushrooms
@RenegadeMushrooms 11 месяцев назад
I add about one pound of grain to every bag. Finished bags weigh about six pounds. Nice smallie 👍
@daveeatmon4718
@daveeatmon4718 10 месяцев назад
@@RenegadeMushroomsthanks! I happen to live next to an awesome lake for smallies. It’s Lake St. Clair in Michigan, great for Walleye and Muskie too!
@nic9779
@nic9779 2 года назад
Love your videos. tape 3 quarters to the rocker to make it a little less annoying and still maintains correct psi
@RenegadeMushrooms
@RenegadeMushrooms 2 года назад
Cool trick, thx for watching. I've actually come to love the rocker swizzle of the presto, but I might be the only one 🤔
@nic9779
@nic9779 2 года назад
Definitely might be the only one 😆 I have been contemplating buying one of the induction plates for awhile to get it out of my kitchen
@RenegadeMushrooms
@RenegadeMushrooms 2 года назад
@@nic9779 Those 1300 watt waring pros I run get the job done. But not alot of juice to spare.
@nic9779
@nic9779 2 года назад
Might have to pull the trigger. I tried a cheap electric single burner I had awhile back and found out real quick that it was not powerful enough kept kicking off
@lucasbogan7103
@lucasbogan7103 Год назад
Nice video.
@RogerJackson-rz1ly
@RogerJackson-rz1ly Месяц назад
Thanks for the video, can you use mixed corn for chickens ? It’s wheat and cut maize . Thanks
@rad7142
@rad7142 2 года назад
thank you for this, you just showed why my grain inocculation did not go well at all. got yeast contam . grain was too wet and some split ....
@RenegadeMushrooms
@RenegadeMushrooms 2 года назад
Split grains greatly increase risk of contams, as do grains that are too wet. That's why I settled on this method, perfect moisture balance and no burst kernels. Hope it works out for you. Thanks for watching 👍
@WhiteBeardHashtag1205
@WhiteBeardHashtag1205 3 месяца назад
Those Microppose hydrophobic adhesive filtration disks are awesome! Ive been using them for about 2yrs now on my PP5 grain jar lids. Being hydrophobic you dont have to ever worry about the filter disks being a vector for contamination. I ordered several sets, because I thought I would be replacing them, but theyve been through dozens of PC runs and are still perfect! I havent had to replace a single one, and not a single one has come off my lids either. I HIGHLY recommend them, I recommend them so much that I promote them in my videos for free. I was unaware that the purge sequence on the PC run is unnecessary, I will definitely try skipping it and see if my tam rate increases. Btw, the foil is completely unnecessary as well. I quit using foil to cover my lids last year, and my tam rate did not increase in the least. Same with gypsum in my sub/grains/LC, quit using it and absolutely nothing changed, I cant for the life of me what its purpose is in this hobby. My tam rate is still virtually zero, and there has been no loss in weight on harvest. I dont see the benefit, so its no longer an additive in my materials. I believe the point you made about light is s fallacy, an old wives tale. I run 2 6500k LEDs in my grow space that the jars are exposed to 12hrs a day, and I never have issues with colonization. I break n shake at 30% (approx 10 days), and achieve full colonization in approx 2.5 weeks. Not saying that it helps, but it definitely doesnt hurt, in my experience. Unrelated to your video, I also learned from a lab tech that agar can be poured at much lower then previously recommended temp. If you are decent at pours, you can actually pour at 100f as opposed to the recommended 130f. Ive adopted this method and have been using it for about 2yrs. I normally pour around 130 plates per run, and the agar doesnt set up on me until after I finish the pour. Pouring at the lower temp reduces both bubbles and condensation on the plate lids.
@RenegadeMushrooms
@RenegadeMushrooms 3 месяца назад
I love the microppose lid gear as well, made my life so much easier. I still use the foil lid covers for my LC. I personally saw an up-tick in contamination without them which I determined was probably a result of a few of my well-used RTV injection ports being so swiss cheesed that they allowed contams in after awhile. For that reason I stuck with them. I use the silicone lid shields from Microppose.com on my grain jars now just to give my lid gear a bit of protection from direct steam, but I remove them after the PC. I also run 6500K LEDs. My limited testing has shown a bit faster growth (mostly at jump off) between dark and direct fruiting light. I have a video on that on my channel. The difference may be so insignificant that it doesn't matter much in the long run, but as I said, my testing has been very limited. Certainly total darkness is not necessary, that notion died long ago, but I still give them a bit of protection from direct fruiting light. I haven't graduated to pouring my own agar yet, but I get what you're saying. I just buy my plates pre-poured. For me it's really just a time issue at this point. I've also noted the same with the gypsum. Sometimes I add it, sometimes I don't, and haven't noticed a difference. I mostly just add it now because I'm trying to use up the giant bag I have.
@WhiteBeardHashtag1205
@WhiteBeardHashtag1205 3 месяца назад
@@RenegadeMushrooms haha! I did that as well, I had a huge bag of gypsum because it was cheaper to buy in bulk. I used it simply because Im such a cheapo I couldnt throw it away and not use it 🤣 You got those silicone lid covers? How do you like them? I have 5 dozen grain jars in rotation, and I saw what it would cost to buy enough for all those lids, and again, Im a cheapo so I didnt pull the trigger. They look really nice though. As far as agar goes, if I had more expendable cash I would definitely sub out my pours. Kids are hella expensive though, and I can only afford to DIY
@RenegadeMushrooms
@RenegadeMushrooms 3 месяца назад
@@WhiteBeardHashtag1205 I here ya, I have my first one about to go to college. As far as time and money go, I don't have much of either 😂 but with agar plates I'm buying my lunch for now. I do like the silicone lid shields. After about 20 or so PC runs they seem like they will last forever. They pop on and off easy like a swim cap.
@tipofthecapmushrooms5523
@tipofthecapmushrooms5523 Год назад
Same, I never vent for any sterilization and works fine.
@thereseayakouame596
@thereseayakouame596 Год назад
Perfect ❤
@troybrigham1434
@troybrigham1434 2 месяца назад
Hi, great tutorial, thank you. What temperature do you keep your jars at while running the mycelium.
@RenegadeMushrooms
@RenegadeMushrooms 2 месяца назад
65 to 70F for me works good for most varieties. If you're growing tropical strains that like it a bit warmer I use one of these: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-wV69bbHtSFg.htmlsi=I0aw2O2nrvcvmHtn
@DrGaryGreen
@DrGaryGreen 2 года назад
Thank you again for all you are sharing. I'm going to ask about Rye. Same hydration as wheat? Handle it the same as wheat?
@RenegadeMushrooms
@RenegadeMushrooms 2 года назад
Basically yes, I haven't done a lot of work with Rye personally but I think it's just a bit slower on the hydration than wheat. The hot water overnight soak still seems to get it nicely hydrated though.
@tonyjohnkoski9482
@tonyjohnkoski9482 Год назад
THANKS ALOT . tony
@lilywoodfarm
@lilywoodfarm 4 месяца назад
Thanks for the great video! I am very motivated and love your method. What was the growth time to full colonization? You gently shook the jars after 7 days, but did not mention the period until colonization was complete. I appreciate this varies, bt a rough guide will suffice. TIA
@RenegadeMushrooms
@RenegadeMushrooms 4 месяца назад
Generally two to four weeks for full colonization with most species.
@lilywoodfarm
@lilywoodfarm 4 месяца назад
Thanks @@RenegadeMushrooms
@chandracox6814
@chandracox6814 Год назад
I've never heard anyone say LC is prone to contam. MSS are way more prone to contam if not put to agar first. This video is great. Very easy tutorial to make some grain jars. Mush love!
@metarox69
@metarox69 Год назад
is prone to contam bacause is a nutritious medium for bacteria, so everytime you use a liquid culture you run a risk, still is the best method to inoculate
@narannavan
@narannavan Год назад
Yeah it is more prone. I test my jars on agar before use
@brianbarton477
@brianbarton477 Год назад
Awesome video once again. I don't have a Blue seal store here any more. I tried looking up Blue Seal wheat grain, I didn't get a hit with any just saying Wheat grain??? Is there a specific name on that bag you where showing? Thanks again
@RenegadeMushrooms
@RenegadeMushrooms Год назад
It's used for chicken feed. If you have a country store like tractor supply around they should have wheat or oat grain in big bags. Either will work, although I prefer wheat. Red or white wheat both work great, just check out the poultry/livestock feed department.
@RobBanks81
@RobBanks81 Год назад
Great video, so much information. Unless I missed it, what temperature range is good for growing out the mycelium in grain spawn? I have a cold house, but I can use a heated seedling mat under a tub if necessary. Thank you.
@RenegadeMushrooms
@RenegadeMushrooms Год назад
65 F is fine if you have a room at that temperature. You have to be careful with heat mats, a little too cold is better than too warm.
@RobBanks81
@RobBanks81 Год назад
@@RenegadeMushrooms Thanks! My basement is in the low to mid 50's this time of year. I'll use the heat mat and set the thermostat to the high 60's.
@kenyamarlow1408
@kenyamarlow1408 2 месяца назад
Nice video! What if I don't have a flow hood? Will this be an issue? Are there alternatives or safe practice I can use without one? Thank you
@RenegadeMushrooms
@RenegadeMushrooms 2 месяца назад
Most people start with a still air box until they can afford to buy or build a flowhood. Check out the "nocbox" from North Spore.
@get1moore
@get1moore Год назад
I'm a newbie to growing mushrooms. I'm almost ready to do my first batch. I'm loving your videos. I'm learning a lot. I have a question. What's the reason for moving the cultured spawn from the jars to the hardwood pellets? After a few weeks, can't you just harvest the mushrooms right from the jars? BTW, I'm in western NY as well.
@RenegadeMushrooms
@RenegadeMushrooms Год назад
Hey there and welcome 👍 Some mushrooms will fruit straight from grain, but your yields will be very low. Grain spawn is really just intended to be a nutrient rich carrier for mycelium, not a fruiting substrate. By moving it to a sawdust or other larger substrate block, your yields will GREATLY increase. Also, most raw substrate material is way cheaper than grain per pound.
@get1moore
@get1moore Год назад
@@RenegadeMushrooms That makes sense! Thanks! BTW, I have accumulated a ton of questions as I've gotten into this. I'll try to ask them one at a time and over the course of time, just so I don't overwhelm you (not that you can't handle the questions). Keep up the great job teaching all of us.
@get1moore
@get1moore Год назад
@Philip Augustine Thanks Philip, I'll check it out.
@eithnebullick1266
@eithnebullick1266 6 месяцев назад
I prepared my first batch of LC from spores and ready for the next step - testing for contamination on agar and then on to inoculating grain. Can you please advise on the types of syringes and needles you recommend including needle gauge size? Disposable or reusable? Luer locks? Thanks so much!
@RenegadeMushrooms
@RenegadeMushrooms 6 месяцев назад
I personally prefer 20cc syringes and 14 or 16 gauge needles, both Luer Loc. You generally get what you pay for, B-D brand stuff is nice. I use disposable.
@faerie_willow
@faerie_willow Год назад
Alright my first attempt went bad, attempting number two after watching your vid 😂🤞🏻
@RenegadeMushrooms
@RenegadeMushrooms Год назад
Moisture balance and a good lid setup are the two keys. I'm sure this attempt will ho much better.
@faerie_willow
@faerie_willow Год назад
Update: we have mycelium! 😁🎉 Your lid video was super helpful too! Thanks!
@brandonpetrisky2058
@brandonpetrisky2058 3 месяца назад
Yea same here 1st attempt ended in a moldy mess. I used a pre made spawn bag I got from online not sure what went wrong. Round 2 is to follow your instructions step by step! Wish me luck and happy growing.
@dylski4294
@dylski4294 Год назад
Do you use the silicon gaskets with the pp5 lids for your grain spawn? I remember you do it for LC but was unsure for grain spawn. Trying to find out if they’re necessary for quart grain jar spawn pp5 lids
@RenegadeMushrooms
@RenegadeMushrooms Год назад
They're not necessary for me as long as I keep the jars in a clean storage tote for incubation, but they definitely seal much better with the gaskets. If you're working in a dirty air environment or incubating in open air, I would definitely use the gaskets.
@frasercoasthomemaintenance7701
@frasercoasthomemaintenance7701 2 года назад
Hi, I love your videos but I cannot find any information on the internet how to introduce the grain spawn to the growing bags and how much grain spawn you need per kg to start the mushroom growing. It would be nice if you can let me know or show a video how to do this. Thanks very much and keep up the good work.
@RenegadeMushrooms
@RenegadeMushrooms 2 года назад
Check out my oyster mushroom growing video, it shows the entire process in detail. Here's a link: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-uyEZLDJ15UE.html I only use pasteurization, I don't sterilize, so I use 1 quart or about 1 lb. of grain spawn per 5 lb. bag of sawdust. My finished fruiting blocks are a little over 6 lbs.
@user-im6zv7vc6i
@user-im6zv7vc6i 2 месяца назад
Awesome video. I didn't catch you say how much LC you are putting inside of the jar or that doesn't matter?
@RenegadeMushrooms
@RenegadeMushrooms 2 месяца назад
For quart jars I usually to 4 ccs or so. You usually get around 12 ccs in a store-bought LC syringe so I like to get three jars done with them. It depends on species though. You would want to go heavier with slow growing species, especially if you're making your own LC.
@serrated0311
@serrated0311 4 месяца назад
Great video while I’m researching proper technique. I’m all for the soaking and pressure cooking, but curious why you don’t boil. Is that dependent on grain type or you do you just skip boiling altogether?
@RenegadeMushrooms
@RenegadeMushrooms 4 месяца назад
I prefer to skip boiling all together because no matter how careful you are, boiling always leads to increased numbers of burst grain kernels. Burst kernels increase contamination risk so I go with methods that minimize them as much as possible. Starting with hot water, and just soaking and monitoring has always worked much better for me. You can adjust your starting water temp and doak time to suit your needs and grain type. Here's a couple more of my videos that might help you out. ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-sPxdyCY0Ys0.htmlsi=-gn5DItm5lvSkRxB ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-AKwpj4Gc700.htmlsi=S0h4EpzFzPOUBgus
@ronaldwilliams6028
@ronaldwilliams6028 2 года назад
Love the videos and advice. However went looking for the Blue Star Wheat Chicken feed. The only one they have in the Blue/Red bag was made of Cracked Corn, Oats, Wheat and called Scartch Chicken Feed. What do I look for. Also could find a wheat only their web site. What I look for or what's it called ? Thanks in advance for your help.
@RenegadeMushrooms
@RenegadeMushrooms 2 года назад
Its Blue Seal, not Blue Star, just want to make sure you're searching the right company. The other thing I've come to realize is that grain availability is very regional. That being said, you should be able to find wheat. Do you have any agricultural feed stores near you? They would know. You just have to be careful buying wheat that is intended to be used as seed. Seed grains are commonly treated with chemicals to aid in germination. You don't want the chemicals obviously.
@elizabethhappiestwhengarde2938
Thanks for all your informative videos! I sterilized some rye grain January 30 (I got a wee bit excited and got ahead of myself). Will the grain still be hydrated enough to inoculate? Also do you have to add air vents in the tote you place the inoculated jars in?
@RenegadeMushrooms
@RenegadeMushrooms Год назад
That's a long time to leave grain, you might want to resterilize it or just make a new batch. I prefer vents in my incubation totes. I just stuff the holes with balls of polyfil.
@elizabethhappiestwhengarde2938
@@RenegadeMushrooms Thanks for getting back to me. Yeah I figured it was too long. It wasn't my intention to wait that long, but you know how life sometimes gets in the way of the things that you like to do LOL
@RenegadeMushrooms
@RenegadeMushrooms Год назад
@@elizabethhappiestwhengarde2938 Indeed 😁
@user-yz6cg3gi2r
@user-yz6cg3gi2r 7 месяцев назад
I have a question I seen in the background on the left side at 12.20 you have glass jugs and I recognized the plastic thing on top that looks like a bubbler and was just wondering what you used those for? I had a few of those that I found and couldn't figure it out so I put a little water in the chambers and used it as a bubbler for a gravity bong worked well but heat and plastic don't get along lol
@RenegadeMushrooms
@RenegadeMushrooms 7 месяцев назад
They are "air locks" used for making homemade wine. I make 6 gallon batches of wine now and then 😁 They prevent air from entering the jugs, but allow CO2 from the yeast to escape.
@theresasteinbacher
@theresasteinbacher 5 месяцев назад
where can i see your video of the different canning jar lid setups for putting in PC. Thanks.
@RenegadeMushrooms
@RenegadeMushrooms 5 месяцев назад
This video shows my favorites, these are the one's I use: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-Wm4znts9f1g.html
@teardowninc4661
@teardowninc4661 6 месяцев назад
Waiting that 10min helped me stop breaking the bottom of my LC jars. Maybe a coincidence,I don’t know.
@bmartin2304
@bmartin2304 8 месяцев назад
A few questions for you sir. How much LC ml do you put in each jar roughly? was the total spawn time 2-3 weeks? How long can your colonized jars last if you want to put it off for a bit, can you fridge them into dormancy? or is it best to colonize the bulk substrate blocks and store those a bit like store bought mushroom kits. Im trying to get a feel for the timeline for the various steps and shelf life on those steps. Thanks! appreciate the content.
@RenegadeMushrooms
@RenegadeMushrooms 8 месяцев назад
3 - 5 ccs for a quart jar of grain typically. You are better off fridging your grain jars than substrate bags because most species are less likely to fruit on straight grain, and jars take up less space. It's still better to use them as soon as possible, but you can hold them off for a bit in the refrigerator. Just don't do that with a tropical species like pink oyster or almond agaricus because fridge temps will kill them. Colonization times for grain and sawdust are typically 2 to 3 weeks with most species.
@davidmitchell3671
@davidmitchell3671 7 месяцев назад
Thanks for this. I have done some grain spawn by putting it in an Instant Pot with excess water for 5 minutes, and immediately releasing the pressure, then air drying it like you show. It seems to give the right amount of moisture without bursting any grain. I hope I didn't miss this in the video, but I was wondering if you see any benefit from adding gypsum or a small amount of sawdust to the grain spawn. Some folks seem to say that a small amount of a future substrate will help the speed of growth in the end. And gypsum for the sulfur. I guess I've done it without gypsum before, too, but I don't think I have done it enough to notice a difference. Others say add 1/2 teaspoon of lime per jar to keep the pH from dropping too low. Any thoughts on that stuff? Thanks again. Very straightforward videos with subtleties on the art explained well.
@RenegadeMushrooms
@RenegadeMushrooms 7 месяцев назад
The only benefit I've seen from adding sawdust to grain is that the sawdust absorbs excess moisture if your grain is too wet. If you're going to add anything, I prefer wheat bran rather than sawdust because the wheat bran will add more nitrogen and increase the yield of your finished blocks while still helping to absorb excess moisture. I show that in this video: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-S38v-5Bi65w.html Other than wheat bran, I generally don't add anything to my grain. Thank you for watching, and for the kind words 👍
@ScottWConvid19
@ScottWConvid19 Год назад
13:04 I love the passive roast 😂
@RenegadeMushrooms
@RenegadeMushrooms Год назад
Could you sense the sarcasm 😁
@RogerJackson-rz1ly
@RogerJackson-rz1ly Месяц назад
Hi , thanks for great video, can you use wheat grains and cut maize , known as mixed corn for chickens, many thanks
@RenegadeMushrooms
@RenegadeMushrooms Месяц назад
If the corn kernels are cracked, I definitely wouldn't use it. You want whole grain kernels. Whole grain wheat and corn mixed would probably work, but it wouldn't be ideal. If you could find just wheat, that would be much better.
@RogerJackson-rz1ly
@RogerJackson-rz1ly Месяц назад
@@RenegadeMushrooms thank you .
@carlfranz
@carlfranz 2 года назад
Do you prefer working with the flow hood to the side? I'm about to build a hood but not looking forward to facing directly into the wind, which seems to be the usual orientation. I'm going to try your suggestion of wheat -- many of my popcorn spawn jars seem to start out ok but then stall and gradually get ugly. I don't see mold until they've gone seriously downhill so I wonder if contamination can develop so gradually?
@RenegadeMushrooms
@RenegadeMushrooms 2 года назад
The side orientation is just the way I've always done it so I'm used to it. Some people prefer it blowing right at you though. Both work, just personal preference I think. Anything wider than 2' and the side orientation wouldn't really work. As far as the spawn goes, yes, it's always a race between the mycelium and the contamination. Even when we PC bacteria survive, just in a weakened state. Popcorn is pretty prone to contamination in my experience. It is one of my least favorite grains. I think you will love wheat.
@jamesb9448
@jamesb9448 Год назад
The reason for having it facing head-on is that even when ‘clean’, the human body is a ready source of contaminants, mostly from commensal organisms. Side-on can be fine, but you’ll just have to be careful that your hand doesn’t come between the flow and the sterile medium. Of course if you don’t want it bowing towards your face you could configure it pointing towards you and down at about 45 degrees. With that configuration you’ll probably also want a screen. As suggested in the vid, the needle is the only thing which is making contact with the media so you just have to flame it and I always also squirt some 70% IPA onto the injection ports a minute before inoculation. I also like LC but am no expert so have some contam issues, but that’s largely from experimenting with recipes. A lovely clear dextrose makes it easy to see contam and it’s a beautiful thing indeed when you have a nice jar with great clouds of mycelium but no turbidity (as that’s the best indicator of contam).
@lovelyspacevideos
@lovelyspacevideos 8 месяцев назад
super video, just one question , why plastic lids over Metal?
@RenegadeMushrooms
@RenegadeMushrooms 8 месяцев назад
They both have their advantages but I like that plastic lids never rust out on me. I also find the plastic lids easier to remove smoothly when I'm dropping agar wedges to grain. That being said, I still mostly use metal lids for my liquid culture because they seal very well and I still have a lot in reserve. If you go plastic for liquid culture, I highly recommend buying lids with a built-in silicone sealing ring like the Masontops Tough Tops lids.
@ranjanty
@ranjanty 2 года назад
Another great video. I learned my lesson when I made 3 different LC jars from the syringe's I got from Gary. I wiped them down with alcohol before putting them in the SAB. The labels disappeared in a instant. Needless to say I was worried about getting the 3 mixed up before I could re-label them. Live and learn. Cordicep update: I put them in a grow tent on the 16th and I just looked and they are colonizing but I see 3 or 4 dark specks which I guess is mold.😞 anyway to save them ? I did everything in front the flow hood but as I mentioned earlier I had to keep going in and out the room to the outdoors. I'm wondering if I could transfer the clean substrate into jars to salvage the grow? Is all lost at this point? Thanks
@RenegadeMushrooms
@RenegadeMushrooms 2 года назад
Where are the dark spots? Are they only at the surface interface with the plastic tub, or scattered around? I'm sure it is mold, but you may be able to save them.
@RenegadeMushrooms
@RenegadeMushrooms 2 года назад
Also, what is the tent setup you put them into?
@ranjanty
@ranjanty 2 года назад
@@RenegadeMushrooms down on the bottom and on the side of the tub.
@ranjanty
@ranjanty 2 года назад
@@RenegadeMushrooms it's a vivo-sun 4x4 tent. No ventilation or lights.
@ranjanty
@ranjanty 2 года назад
I even sealed the lid with 3M micropore all around the edges.
@ForsakenCrimmy
@ForsakenCrimmy 2 месяца назад
What were the carboys and airlocks for? Just noticed it in the background and was curious.
@RenegadeMushrooms
@RenegadeMushrooms 2 месяца назад
I make homemade wine and hard cider. That's a six gallon batch of blood orange sangria.
@ForsakenCrimmy
@ForsakenCrimmy 2 месяца назад
@@RenegadeMushrooms I like you. Lol. I'm growing pink lemonade blueberries and hoping to have enough this year to make a melomel with it. Appreciate the reply.
@tonyjohnkoski9482
@tonyjohnkoski9482 Год назад
on 9-12-22 i used my 6 qrt.pressure cooker to process 1 bag of rye grain , i just wraped the top of the bag around the grain and covered up the filter patch ; when i took it out of the p.c. the bag was loose and had a lot of condensation inside . can i put the grain into a towel to dry if again and the refill the bag and process it with the filter patch uncovered this time. ? thanks .please a.s.a.p.
@RenegadeMushrooms
@RenegadeMushrooms Год назад
That's why I don't like bags for spawn in small scale pressure cookers, more often than not it turns out badly. I always use jars. You can reuse the grain as long as you don't have a ton of burst kernels. Just get the moisture content right and repackage it. If there are a lot of burst kernels, you need to start over. Burst kernels lead to contams big time.
@MathieuGoldstein
@MathieuGoldstein Год назад
That’s very interesting, thank you. I used to soak then put my grain in boiling hot water and just let it sit heat turned off for about 30min. This way the grain gets extra plump (the light cooking makes it suck more water than just the initial soaking. It used to work just fine, yet since a few weeks I’m running into sour rot contamination problem which had me ditch so many grain jars. I am then switching to your technique to put a dryer grain into my grain spawn jars. Let’s hope I get rid of this awful bacteria 🤞
@RenegadeMushrooms
@RenegadeMushrooms Год назад
Hopefully it helps, losing jars is a real bummer.
@momsmushroomsjodyfoster5786
@momsmushroomsjodyfoster5786 4 месяца назад
Random question, is the term field capacity refer to grains or only substrates of wood, manure, straw ect.? Just for my own information. Thx
@RenegadeMushrooms
@RenegadeMushrooms 4 месяца назад
That's a good question. Off the top of my head, I have to say I've mostly heard it used in regard to substrate hydration vs grain in mycology circles. Always referring to hydrating a substrate to it's optimum moisture content.
@bobcat7380
@bobcat7380 Год назад
If you don't use all your lc do you have to take off the needle and put the luer lock tips back on or can you just leave needle and cap it
@RenegadeMushrooms
@RenegadeMushrooms Год назад
Just leave the needle cap on and store it in a ziplock bag in the fridge.
@danlee7376
@danlee7376 Год назад
. 1 question that I have is What I have going on is freshly planned Hardwood Sawdust. Do I have to prepare the sawdust Before I Add from the jars. .
@RenegadeMushrooms
@RenegadeMushrooms Год назад
I only use hardwood fuel pellets, but I think you want to let fresh sawdust age for a couple of weeks before using for substrate. If you have Facebook, ask at the "Mushroom Growers for Beginners and Experts" FB group.
@dylandepuy3715
@dylandepuy3715 Год назад
Your lids have self healing injection ports but is there nothing to allow for gas exchange?
@RenegadeMushrooms
@RenegadeMushrooms Год назад
9:45 Microppose injection port and filter disc in every lid.
@donjohnson7189
@donjohnson7189 Год назад
I'm thinking on getting into growing mushrooms now having said that I'm into making wine,beer and "other" form of alcohol this also needs to be sanitized and I use "Star sand" do you think I can use this as a sanitizer for this new endeavor?🤔
@RenegadeMushrooms
@RenegadeMushrooms Год назад
I actually was just reading a discussion on this very topic on FB and I also did some research on Shroomery. From what I've read, Starsan is far less effective than 70% isopropyl and not recommended for mycology. Some people have tried it on not been happy with the results.
@donjohnson7189
@donjohnson7189 Год назад
@@RenegadeMushrooms cool thanks for the quick response.😉
@chascodelviso
@chascodelviso Год назад
What temperature do you shoot for when incubating the jars?
@RenegadeMushrooms
@RenegadeMushrooms Год назад
60 - 70 F
@dylandepuy3715
@dylandepuy3715 Год назад
Would you do anything differently if you were using cracked corn instead?
@RenegadeMushrooms
@RenegadeMushrooms Год назад
I wouldn't recommend cracked corn, it's pretty prone to contamination. If you're looking for cheaper options, bird seed works much better. Especially if you find it without the sunflower seed in it. Wheat and oats are cheap and you can find them at most farm and feed stores and they work great. Just my 2 cents.
@mikewaldrop4977
@mikewaldrop4977 Год назад
After transferring grain spawn to my sterile strata do I have to have an air filter on meh poly bag?
@RenegadeMushrooms
@RenegadeMushrooms Год назад
The bag needs to breathe so yes you either need an air filter or breather holes in the bag. As a general rule, for sterilization with supplemented substrates, you need a filter patch bag. For pasteurized grows with unsupplemented substrate, you can just poke holes in any old bag or container, or use a filter patch bag if you wish.
@kacydavis3767
@kacydavis3767 7 месяцев назад
Do we loosen the jars while in the pressure cooker and c9ver with foil?
@RenegadeMushrooms
@RenegadeMushrooms 7 месяцев назад
Don't loosen the lids, but cover them with foil lids or I also like the silicone lid shields from Microppose.com.
@LifeWaytv4287
@LifeWaytv4287 Год назад
🇰🇪 Very insightful.✅
@whodidit99
@whodidit99 2 месяца назад
If you used spores instead of LC would you squirt it in the jar differently?
@RenegadeMushrooms
@RenegadeMushrooms 2 месяца назад
No, maybe just add a little more because of the germination lag. Honestly it's a waste to squirter spores on to grain though. Make your spores into LC, expand it, then LC to grain. Pennies on the dollar.
@guybuckland4404
@guybuckland4404 Год назад
Is there a reason you don't use the metal lids with inoculation ports, etc.?
@RenegadeMushrooms
@RenegadeMushrooms Год назад
Metal lids work fine, but they rust eventually and are tougher to drill holes in. I still use metal for liquid culture, but plastic for everything else.
@mattnsim
@mattnsim 2 года назад
I find I get far less contamination in jars than bags. I work in front of a flow hood too..
@840swed840
@840swed840 Год назад
Kinda noticed this also, maybe it’s because you eliminate any issues that can come with the lid/self healing injection port
@connormurphy3085
@connormurphy3085 Год назад
that’s mainly due to the fact that bags are full of seams and areas where contam can get in vs jars where there are only one or two possible areas where contam can be introduced
@TeteKodjo
@TeteKodjo Год назад
Merci je le vidéos en Français svp
@arabiclearner711
@arabiclearner711 Год назад
Maybe you should try sterilizing for longer times with bags, I have heard many recommend it and it makes since that they would require it
@oldschoolman1444
@oldschoolman1444 Год назад
It takes longer to get to the proper temperature due to the bulk, takes longer for the middle to get hot enough.
@jcondron
@jcondron Год назад
Assuming you refrigerate your spawn jars, how long can you wait between PC and inoculation?
@RenegadeMushrooms
@RenegadeMushrooms Год назад
It's best to use them asap, but I would say two weeks tops.
@nerdoutreachprogram5358
@nerdoutreachprogram5358 Год назад
I never leave the rocker weight off. I leave it on the whole time and everything continues to work fine.
@aarongentile7232
@aarongentile7232 6 месяцев назад
great video!!! LOL @ "wetter is better" comment
@seanpinnell4806
@seanpinnell4806 10 месяцев назад
Can you store fully colonized grain jar spawn in fridge, with the intention to be taken out later to inoculate substrate?
@RenegadeMushrooms
@RenegadeMushrooms 10 месяцев назад
Yes, unless it's one of the tropical strains that can't tolerate fridge temps like pink oyster.
@lisaamirant5595
@lisaamirant5595 8 месяцев назад
I just got jars exactly the size in this video and they are almost ready, but I’m not sure what to do next. My first ones were in small jars that I “birthed” and kept cakes. Do you do the same with larger grain spawn jars? I can’t find any info on what to do next. Thank you🙏
@RenegadeMushrooms
@RenegadeMushrooms 8 месяцев назад
You can fruit some species straight from grain, but usually it's best to mix large volumes of grain into a prepared fruiting substrate like sawdust, compost, etc. Research what substrate your particular species prefers to fruit on and go from there. As I said, some will fruit from grain, but grain is typically best used as a "carrier" for your mycelium to inoculate a larger volume of substrate.
@lisaamirant5595
@lisaamirant5595 8 месяцев назад
@@RenegadeMushrooms so from the jar into maybe a plastic shoe box? Then does it need heat and light and to be covered for moisture? I really appreciate your advice 🙏
@RenegadeMushrooms
@RenegadeMushrooms 8 месяцев назад
@@lisaamirant5595 I typically use plastic containers or model 3T filter patch bags from Unicorn Bags for my fruiting substrate. The substrate you use depends on the species you are trying to grow, but yes you will need to seal the bag or cover the container to maintain humidity.
@lisaamirant5595
@lisaamirant5595 8 месяцев назад
@@RenegadeMushrooms thank you
@BettyHensley-bk5rm
@BettyHensley-bk5rm Год назад
I have stored wheat that is several years old. Can I use this older wheat for this process?
@RenegadeMushrooms
@RenegadeMushrooms Год назад
As long as it's not moldy, go for it.
@neilfarmer08
@neilfarmer08 6 месяцев назад
The reason you leave the rocker off, to vent it making sure there's no blockage. You can accomplish the same thing by sicking through the vent. You'll know if it's clear.
@RenegadeMushrooms
@RenegadeMushrooms 6 месяцев назад
I guess that makes sense if you're doing bags in the PC, or something else that could potentially expand and plug your vent. Another reason I prefer and always recommend jars for people with small PCs. As far as being plugged with debris, I always just look through mine.
@elizabethr2908
@elizabethr2908 6 месяцев назад
Can I use this same method with oats? Great video!❤
@RenegadeMushrooms
@RenegadeMushrooms 6 месяцев назад
Yes, this will work with oats 👍
@elizabethr2908
@elizabethr2908 6 месяцев назад
@@RenegadeMushrooms , awesome! Thank you, I’m learning so much from your epic channel! I’m starting my oat soak now. Thanks so much for your speedy reply ❤️
@RenegadeMushrooms
@RenegadeMushrooms 6 месяцев назад
@@elizabethr2908 Glad you are enjoying the channel. Remember to soak your grain until you can push your fingernail into a kernel and easily compress/crease it with your nail. It may take up to 15 hours, but you can add more hot tap water half way through to speed things up if you want. Also remember to let it air dry for a few hours before packing it into your jars. Good luck!
@brianp91
@brianp91 Год назад
New to mushrooms and I really like and appreciate your videos. I am having a terrible time getting my grain spawn to grow. Every batch is contaminated(10). I wash hands, glove and mask up, 70% alcohol hands, jars, surfaces, flame needle then wipe with completely soaked paper towl in alcohol. Not sure what else I can do. I am using rye berries and after 4hrs drying they are not Moises to the touch. After I use PC I can see moisture in jars. Dried again and did PC 2nd time and still a little moisture on glass jar. I even turned off house ac 30 min prior to inoculation. Ideas? Thank you for your time! Brian
@RenegadeMushrooms
@RenegadeMushrooms Год назад
Moisture in the jar after the PC run is normal, even with properly prepared grain so don't sweat that. It will dissipate after a good shake and a few days time. That is not causing your contamination. Some questions: 1. How long do you PC for and at what pressure? 2. What lid setup are you using on your jars? 3. Where do you get your liquid culture, and is it all one batch or multiple species?
@brianp91
@brianp91 Год назад
@RenegadeMushrooms PC for 90min at high which is 12 psi and I am at sea lvl. I grew my own liquid culture with no problems so that's why I think the PC 12psi is not the issue? Lids I have tried multiple. High temp sealant with another hole for air with a filter, 3m breathable tape. I also got the disks that can be injected through but that round doesn't let jar breathe. When injecting LC it sucked it right into jar. It's all one batch not multiple strains.
@RenegadeMushrooms
@RenegadeMushrooms Год назад
@@brianp91 Gotcha, well if you're going to use breathable tape for your filter, make sure you use two layers of it. I like the little filter discs I show in the video, but lots of people use two layers of tape with good success, just make sure you have some type of lid filter. If all of your jars contaminated, the two likely culprits are: 1. Contaminated batch of LC 2. Incomplete sterilization If I was a betting man, I'd put my money on contaminated LC.
@brianp91
@brianp91 Год назад
@@RenegadeMushrooms what does contaminated LC look like? Mine is clear with the cloud of spores
@RenegadeMushrooms
@RenegadeMushrooms Год назад
@@brianp91 That's the one drawback to LC vs. agar. It's much more difficult to visually see contaminants. Did you make it from a spore syringe? Also, are you getting mold or bacterial contamination?
@dylski4294
@dylski4294 2 года назад
What size holes do you have drilled in the sides of your tote? The ones you have poly-fil stuffed in
@RenegadeMushrooms
@RenegadeMushrooms 2 года назад
1.5 to 2 inches works well. I use a hole saw. Make sure you press gently though so you dont crack the plastic.
@dylski4294
@dylski4294 2 года назад
@@RenegadeMushrooms Thanks broseph
@RenegadeMushrooms
@RenegadeMushrooms 2 года назад
@@dylski4294 NP broosevelt
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