The simplest estimate of IC50 is to plot x-y and fit the data with a straight line (linear regression). IC50 value is then estimated using the fitted line, i.e., Y = a * X + b, IC50 = (0.5 - b)/a.
MTT assay to determine the IC50 value of the different drugs and analyze their effect on cell viability. ... The IC50 value (that is, the concentration of drug which exhibited 50% cell viability for MCF-7 and MDA-MB-231 cells) were 15, 15, 10 μM, and 100 nM, respectively, for AZA, SAM, SFN, and TSA, respectively.
The half-maximal inhibitory concentration is a measure of the potency of a substance in inhibiting a specific biological or biochemical function
11 сен 2024
