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How to calculate LOD and LOQ by different ways 

Dr.Abdelkhabeer
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How to calculate LOD and LOQ by different ways?
• 6-How to calculate LOD...

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22 авг 2024

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Комментарии : 107   
@AmiraMohamed-xr8dg
@AmiraMohamed-xr8dg 2 месяца назад
Thank you very much for the well-organized and step-by-step guidance for method validation. I appreciate it much.
@AccreditedLaboratory
@AccreditedLaboratory 2 месяца назад
Thank you very much. The previous video had poor quality due to its age. Please check this updated link. ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-kThlQO1qHKc.htmlsi=cHalldMr_nYzHEoj
@AccreditedLaboratory
@AccreditedLaboratory 2 месяца назад
The following link in arabic ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-7g_twN60TvM.htmlsi=VStiBT7LUjOFhrE4
@jeddaaah
@jeddaaah 5 лет назад
اتمنى انك تستمر بالفيديوهات.. سجلت ك مشترك جديد لقناتك.. اعجبتني مستوى لغتك في الانجليزي.. لانه نادر جداً احصل اخ من مصر الحبيبه يتكلم انجليزي بشكل جيد.. استمر يابطل
@AccreditedLaboratory
@AccreditedLaboratory 5 лет назад
بأمر الله جزاك الله كل خير
@kinghob6647
@kinghob6647 3 года назад
احسنت انت رائع اتمنى منك تزويدي بمحاظراتك باللغة العربية للأستفادة وجزاك الله خيرا
@AccreditedLaboratory
@AccreditedLaboratory 3 года назад
جزاك الله خيرًا فيه بعض المحاضرات باللغه العربية ولكن المعظم بالإنجليزي لأنه مطلوب أكتر في مجالنا خد جولة كده في القناة هتلاقي إن شاء الله فيديوهات كتير مرتبة
@kinghob6647
@kinghob6647 3 года назад
اشكرك استاذ والله يحفظك ويمن عليك بالصحة والسلامة ..... هل توجد لديك صفحة على الفيس او واتس اب للتواصل معك لطرح بعض الاسئله ان امكن اني اعمل في مختبر ونحن في طور التأهيل ولدي بعض الاسئله فهل يمكنك مساعدتي والله يحفظك استاذ
@AhmedAbouHalima
@AhmedAbouHalima Год назад
الله ينور عليك يا دكتور محمد.
@AccreditedLaboratory
@AccreditedLaboratory Год назад
ده قديم أوي معلش إسمع ده أفضل بالعربي ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-7g_twN60TvM.html
@AhmedAbouHalima
@AhmedAbouHalima Год назад
@@AccreditedLaboratory مفيش مشكله. انجليزي حضرتك كويس وقادر أفهمه. ياريت تشرفني بقناتي علي شغل Spectroscopy and chromatography instruments
@AccreditedLaboratory
@AccreditedLaboratory Год назад
Please follow the advanced completed training course for method verification & validation with a high quality audio and video ru-vid.com/group/PLyMlCjuRlnXE4F6QIVO4ZvhUcc5T6wqIs
@tayeebatarannum
@tayeebatarannum 3 года назад
Thank you. May Allah give barakah in your knowledge.
@AccreditedLaboratory
@AccreditedLaboratory 3 года назад
Thank you so much
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Full method validation follow this link Method Validation ru-vid.com/group/PLyMlCjuRlnXEdUbYVyeVZrflxBNdbXHmS
@articles2757
@articles2757 3 года назад
Good work sir, Keep going please
@AccreditedLaboratory
@AccreditedLaboratory 3 года назад
Thank you
@munawarahmed9674
@munawarahmed9674 4 года назад
Salam Mr. KhabeerThank you very much, very well explained, keep it upGod bless you
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Munawar Ahmed Thank you so much... This is playlist for whole method validation in sequence ru-vid.com/group/PLyMlCjuRlnXEdUbYVyeVZrflxBNdbXHmS
@Kirov_reporting_95
@Kirov_reporting_95 2 года назад
Many thanks for this video, I am preparing for a job interview at the moment and your channel has been very helpful
@AccreditedLaboratory
@AccreditedLaboratory 2 года назад
You don’t know how much this comment makes me happy thank you
@Kirov_reporting_95
@Kirov_reporting_95 2 года назад
@@AccreditedLaboratory I got the job, I am now working in a lab doing method development. Your videos have been incredibly helpful in helping me learn quickly what I need to know for the role. Many thanks my friend :)
@AccreditedLaboratory
@AccreditedLaboratory 2 года назад
Alhamdollah my channel not from the top scientific channels but if my lectures will help someone to get a job that will be enough for me. Thank you so much for your follow and support and I wish I can help thousands of people to get a job and improve in their job
@vaidehikatoch8619
@vaidehikatoch8619 Год назад
Well explained sir Can we use this method in raman spectra as Iam facing a problem in LOD and LOQ in raman?
@AccreditedLaboratory
@AccreditedLaboratory Год назад
Listen to this playlist Method development & validation (High Quality) ru-vid.com/group/PLyMlCjuRlnXE4F6QIVO4ZvhUcc5T6wqIs
@AccreditedLaboratory
@AccreditedLaboratory Год назад
This can be applied for the method itself with any instrument
@samimaktharshaik2697
@samimaktharshaik2697 3 года назад
Alhamdulillah 😀, it is help full
@AccreditedLaboratory
@AccreditedLaboratory 3 года назад
Alhamdullilah, thank you
@KingSuleupdates
@KingSuleupdates Год назад
Thanks a lot
@AccreditedLaboratory
@AccreditedLaboratory Год назад
This is higher quality ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-kThlQO1qHKc.html
@AccreditedLaboratory
@AccreditedLaboratory Год назад
This is in Arabic ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-7g_twN60TvM.html
@nadiyataha8533
@nadiyataha8533 9 месяцев назад
Could you please explain the effect of standard curve on high quality control and low quality control
@AccreditedLaboratory
@AccreditedLaboratory 9 месяцев назад
Do you mean if the calibration curve is not accepted or slope of the curve not within the acceptance limit
@toninogerber3103
@toninogerber3103 3 года назад
This video is great, it helped me a lot. Thanks for that!
@toninogerber3103
@toninogerber3103 3 года назад
You earned my subscription even for just the first explanation of the difference between LoD and LoQ, very nice and clear declaration!
@AccreditedLaboratory
@AccreditedLaboratory 3 года назад
It’s my pleasure brother thank you so much for your concern
@AccreditedLaboratory
@AccreditedLaboratory Год назад
Announcement, English Through this link, you can enroll in a comprehensive course on Method Validation, Verification and measurement uncertainty Calculations in English. The course comes with guidelines and an Excel sheet with calculations that you can download and utilize for practical applications. For all my followers and those who are new to the channel, you will find the complete course exclusively available here. Additionally, any future updates to the course will be accessible to you, allowing you to continue benefiting from it. At the end of the course, you will receive a certified certificate from Udemy. I want to emphasize that this course is exceptionally well-explained, making it straightforward and accessible. Many individuals have expressed their gratitude for how it has helped them in their personal endeavors and professional development. Best of luck to all, Insha'Allah! www.udemy.com/course/method-validation-based-on-iso-170252017-requirements/?referralCode=103AAC39019691D0F9F0
@mohammadseddiqmortazavi3872
@mohammadseddiqmortazavi3872 3 года назад
hello thank you for presentation
@AccreditedLaboratory
@AccreditedLaboratory 3 года назад
Most welcome brother
@hunderafiromesa4541
@hunderafiromesa4541 4 года назад
Keep it up
@MohamedMahmoud-gs1jo
@MohamedMahmoud-gs1jo 4 года назад
Thanks so much for your video :)
@jamalkhan2305
@jamalkhan2305 Год назад
If you are injecting 2 ppb conc. for both LOD and LOQ then how you determine which one is LOD and LOQ? Can you please care to assist to rectify this confusion? Thank you.
@AccreditedLaboratory
@AccreditedLaboratory Год назад
That’s why I said to select LOQ and from that you will calculate standard deviation that will be used to calculate LOD from 3*S USE THIS LINK ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-kThlQO1qHKc.html
@shohrabalam5704
@shohrabalam5704 5 лет назад
Very good lecture...sir....
@AccreditedLaboratory
@AccreditedLaboratory 5 лет назад
Thanks for your concern
@articles2757
@articles2757 3 года назад
Thank you for making this video. Is Limit of quantification and quantitation is the same? What is MDL (Method detection limit), surrogate standards, internal standards, and mixed standards? Can you please address these questions?
@AccreditedLaboratory
@AccreditedLaboratory 3 года назад
Most of answers for your question you can find here ru-vid.com/group/PLyMlCjuRlnXG-zm5vhknFsL6eMZZdjVCm
@AccreditedLaboratory
@AccreditedLaboratory 3 года назад
and this is internal standard ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE--xDxBV0Rwdo.html
@adolfxavier4858
@adolfxavier4858 Год назад
Thank you ❤
@AccreditedLaboratory
@AccreditedLaboratory Год назад
Most welcome, please find this playlist with high quality ru-vid.com/group/PLyMlCjuRlnXE4F6QIVO4ZvhUcc5T6wqIs
@ashish030394
@ashish030394 4 года назад
Thanks sir
@krishnapriyatk9877
@krishnapriyatk9877 3 года назад
Sir.. Is it correct to have limit of detection value higher than the experimentally done minimum concentration in Stern volmer plot?? I am getting like that. How can I correct that?
@AccreditedLaboratory
@AccreditedLaboratory 3 года назад
Can you please clarify the question
@kishorsambhana7791
@kishorsambhana7791 4 года назад
Nicely explained, ca u give more examples for related substance impurities LOD LOQ calcutaion. like in one drug product three impurities present, then how to calculate
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
kishor sambhan Thank you, can you please contact me here to give you that example facebook.com/Accredited-Laboratory-2285343028361472/
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Also this is the list for method validation Method Validation ru-vid.com/group/PLyMlCjuRlnXEdUbYVyeVZrflxBNdbXHmS
@didiibra84
@didiibra84 2 года назад
What is the guidline or referance that say that lod equal blank+3SD and that Sd calculated from recovery of expected LoQ
@AccreditedLaboratory
@AccreditedLaboratory 2 года назад
In all guidelines you can find how to calculate LOD AND LOQ separated but you should understand how to apply in the real validation because in each you should select the lowest concentration. So, better to select the lowest conc can be quantified accurately by your instrument and method which is LOQ, then to get the S from that and apply it to LOD equation
@HassanAhmed-hq4zx
@HassanAhmed-hq4zx 3 года назад
You did a mistake at the 7 minute in the video... LOD=mean blank+3Sd of the blank(that's the right ) You said LOD=blank +3SD of the 6 replicate of the lowest concentration detected (that's wrong)
@AccreditedLaboratory
@AccreditedLaboratory 3 года назад
Thank you for your interest. In validation you need to calculate standard deviation for all replicates then you will calculate LOD using this SD by this equation. So it should be replicated if blank sample spiked with known concentration not only blank or how you will get SD
@gosaidhruval5935
@gosaidhruval5935 4 года назад
sir please give me all your video link regarding LCMSMS and GCMS i am new learner of this instrument so you help me.......
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Playlist for LC MS/MS ru-vid.com/group/PLyMlCjuRlnXEeiMwLE-wuW_JQoruiVK8m Still not completed but near I will start again after ISO lectures
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Method validation lectures ru-vid.com/group/PLyMlCjuRlnXEdUbYVyeVZrflxBNdbXHmS
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Measurement uncertainty lectures ru-vid.com/group/PLyMlCjuRlnXExllF3CG4S1yXFIeJzPADJ
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
ISO 17025 lectures ru-vid.com/group/PLyMlCjuRlnXHdBY3bfW6Ftt91KdjlIVHS
@humeeayisha3199
@humeeayisha3199 4 года назад
Thanks you
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Most welcome, don’t miss this playlist Liquid chromatography mass spectrometry ru-vid.com/group/PLyMlCjuRlnXEeiMwLE-wuW_JQoruiVK8m
@shadishd1514
@shadishd1514 4 года назад
I think you made a mistake at 20:42 the first x is the indivual result and the second x is de average. Other than this the video is very informative thank you.
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Thank you so much, I will check that and go back to you inshaalah
@shadishd1514
@shadishd1514 4 года назад
@@AccreditedLaboratory habibi no problem 🙂
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Shadi Shd just give me the time exactly if you please
@shadishd1514
@shadishd1514 4 года назад
@@AccreditedLaboratory 6:07
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Yes you are right
@ghpharmacy
@ghpharmacy Год назад
لم لا يكون الشرح بالعربي؟ طالما هو موجه لناس عرب بالطبع فلم لا يكون بلغتنا الاصلية؟
@AccreditedLaboratory
@AccreditedLaboratory Год назад
مين قال أنه موجه للعرب الشرح لكل الناس وعامة دي فيديوهات عربي ru-vid.com/group/PLyMlCjuRlnXG-zm5vhknFsL6eMZZdjVCm
@AccreditedLaboratory
@AccreditedLaboratory Год назад
ru-vid.com/group/PLyMlCjuRlnXGehEbCQZSLGCs3LWLS92Hc
@AccreditedLaboratory
@AccreditedLaboratory Год назад
ru-vid.com/group/PLyMlCjuRlnXGrhAaQUqpPu-rLjJPGh5B7
@paulec6629
@paulec6629 4 года назад
hello, maybe you can help me with the name of some book or paper where they use the third method (use of degrees of freedom) .. it would be of great help
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Can you please contact me on Facebook page?
@HassanAhmed-hq4zx
@HassanAhmed-hq4zx 3 года назад
I think that recovery range is from 98%-102%, How did you get this limit (70-120%)?
@AccreditedLaboratory
@AccreditedLaboratory 3 года назад
This range depend on the guideline used or as listed in the method or depend on the matrix. This range in Eurachem guidlines and in most of food residue analysis methods
@Al3k5and3r
@Al3k5and3r 4 года назад
Hello. What do you think about ICH recommendation about calculating LOD and LOQ from the equation of regression based on fortified samples?
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Thank you so much for your comment, can you please give me example about your query?
@Al3k5and3r
@Al3k5and3r 4 года назад
@@AccreditedLaboratory there is a sheet database.ich.org/sites/default/files/Q2_R1__Guideline.pdf and there is some docs www.cysonline.org/article.asp?issn=2229-5186;year=2011;volume=2;issue=1;spage=21;epage=25;aulast=Shrivastava and some examples: www.uttv.ee/naita?id=24440
@Al3k5and3r
@Al3k5and3r 4 года назад
@@AccreditedLaboratory SO, the main idea is that if I want to calculate LOD or LOQ for METHOD (its important) I should make fortified samples on 5 different concentrations (lowest as possible) with some replications. And then I should make an anlytical procedure with that samples. After that I will obtain results like dependency of signal from concentration (recovery should be over 70% for all samples). Then we calculating regression function and defining the linear range. The error of our regression function will be the complex error of the method. And then we calculating LOD like 3.3 times error of regression equasion devided on slope of function. LOQ - the same but 10 times. The physical sence of it that our LOD and LOQ ever would be grater than any error of method in linear (or close to that) range with recovery over 70% as it should be.
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Can you please message me on this link facebook.com/Accredited-Laboratory-2285343028361472/
@MohamedMahmoud-gs1jo
@MohamedMahmoud-gs1jo 4 года назад
Can you please recommend me a good training provider for HPLC, I want to have deep HPLC training in Germany!
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Mohamed Mahmoud in Germany you can find many companies providing this course like Agilent or ABSCIEX. This is playlist for LC MS/MS lectures to be continued inshaalah ru-vid.com/group/PLyMlCjuRlnXEeiMwLE-wuW_JQoruiVK8m
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Mohamed Mahmoud Inshaalah after I will finish this course, I will start HPLC
@HassanAhmed-hq4zx
@HassanAhmed-hq4zx 3 года назад
ايه المقصود بال maximum residual limit?
@AccreditedLaboratory
@AccreditedLaboratory 3 года назад
Rejection limit. If you get a result which is higher than this limit. So, sample will be rejected
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
Liquid chromatography mass spectrometry ru-vid.com/group/PLyMlCjuRlnXEeiMwLE-wuW_JQoruiVK8m
@HassanAhmed-hq4zx
@HassanAhmed-hq4zx 3 года назад
In the third approach, how L.O.Q is 0.13, and you said firstly it is 0.2ppb?
@AccreditedLaboratory
@AccreditedLaboratory 3 года назад
Yes it’s 0.2 not 0.13
@sarathbabumanda7277
@sarathbabumanda7277 3 года назад
@@AccreditedLaboratory what is the limits of LOD N LOQ
@PrabaCaptain
@PrabaCaptain 2 года назад
Can you tell the exact reference for lod calculation using replicates
@AccreditedLaboratory
@AccreditedLaboratory 2 года назад
In all guidelines but I said in the beginning I use EURACHEM
@articles2757
@articles2757 3 года назад
What do you mean by 3SD? you mean multiple 3 with SD? or add 3 different SD? Its confusing
@AccreditedLaboratory
@AccreditedLaboratory 2 года назад
Multiple
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-HBZbT95LrkQ.html
@AccreditedLaboratory
@AccreditedLaboratory Год назад
Please follow the advanced completed training course for method verification & validation with a high quality audio and video ru-vid.com/group/PLyMlCjuRlnXE4F6QIVO4ZvhUcc5T6wqIs
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-CoIzD9sMNOI.html
@AccreditedLaboratory
@AccreditedLaboratory 4 года назад
ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-4BDz0hDbp-A.html
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