How to Design, Run, and Optimize A Viral Gene Delivery Experiment -

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Learn how to do your own viral gene delivery project: from viral vector selection to delivery
You want to express a gene, but when it comes to choosing the optimal viral vector, your choices can seem overwhelming. Do you go with lentivirus or AAV? Or perhaps adenovirus or retrovirus? Each viral system has its own set of advantages and disadvantages-we'll be breaking it down for you in this upcoming webinar so you can easily pick the right one for the job.
We'll also guide you through the complete workflow, with tips on experimental design to safe and effective delivery of your virus to your cell of interest.
You'll learn about:
- Types of viral vectors and their properties
- How to choose the optimal viral vector
- Designing the right system: promoters, reporters & tags
- How to safely package your virus
- How to optimize your viral infection: calculating titers and MOIs
Links to additional resources mentioned in the webinar:
- Choosing a Viral Vector: Vector Selection Tool - www.abmgood.com/vector-produc...
- Online Titer Calculator (under "Titer Calculator" section on the page) - www.abmgood.com/High-Titer-Le...
- Lentivirus Packaging Protocol - info.abmgood.com/hubfs/Lentiv...
- Lentivirus Infection Protocol - info.abmgood.com/hubfs/Lentiv...
- Knowledge Base and Videos - www.abmgood.com/marketing/kno...
- Viral Vectors Blog Posts - info.abmgood.com/blog/topic/v...
- abm's Genome-wide Vector and Virus Collection - www.abmgood.com/Vectors.html
As thank you for attending our webinar, here's your exclusive promocode for an additional 10% OFF the lowest advertised price on abm vector and virus (lenti-, retro-, AAV, adenovirus) products:
Apply promocode: Web-Vector10 at checkout, or let your account representative know to claim your discount.*
*For North American customers only, some restrictions may apply.
➜ Can't make it live? Sign up to receive a recording of the webinar instead: info.abmgood.com/viral-vector...
Connect with us on our social media pages to stay up to date with the latest scientific discoveries:
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Опубликовано:

18 июн 2019

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Комментарии : 14

@mahnazmehrabi7626 2 года назад

Very nice webinar

@adwoabiotech 4 года назад

The audio was hard to hear but the content was excellent ⭐⭐⭐

@abmgood 4 года назад

Hi Adwoa, thanks for watching our video and leaving such a nice comment! We are always working to improve our audio quality - thanks for your feedback!

@smoothhunterz 5 лет назад

30:20: 10 million in 10^5 is 100,000 not 1 million. 31:16: What is the virus titer for respective cell lines for those suggested MOIs. Do all those cell lines have uniform virus titer?

@abmgood 5 лет назад

Hi Saikumar, sorry about the confusion for your comment at 30:20. Our speaker, Michael, was providing a verbal example that was different from the one on the right of the screen. (He goes on to cover the displayed example on the right shortly afterwards.) As for your question, it doesn't matter what the titer is for respective cell lines, as it is a calculation of titer vs the amount of cells. You can adjust the amount of cells with whatever titer you obtain. A titer of 10^7IU/mL is the most common, however.

@smoothhunterz 5 лет назад

@@abmgood Thank you for your reply, for example, MRC5 has MOI 1, PC12 has MOI 20 and when they were tested from 1 to 30 MOI. How do we discern differences between 1 and 2 MOI for MRC5, 20 and 21 MOI for PC12. Will a change in one MOI be cytotoxic to the cells?

@abmgood 5 лет назад

@@smoothhunterz A change in 1 MOI will not cause the virus to immediately be highly cytotoxic - this is more of a gradual cytotoxicity induction. we choose the MOI that shows best infectivity without showing cytotoxicity. It could be that an MOI of 5 OR 10 for a cell line won't cause cytotoxicty, but have similar infectivity - in these cases, we choose the lower MOI as to reduce the amount of virus we use, whilst obtaining similar results. Hope this helps!