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Even if you silence the tumor suppressor genes, wouldn't the finite length of the telomeres still limit the immortalization if you use only that method?
Could you differentiate between the tranfer plasmid with and the envelope plasmid? For example, if I want spike proteins on the pseudoviruses , to which plasmid( transfer plasmid or envelope plasmid ) should I clone the sequence for spike protein?
Thank you for the comment! The transfer plasmid would carry the genetic material that the pseudovirus will deliver into the target cells, but it does not control which proteins appear on the surface of the pseudovirus. To have spike proteins on the surface of your pseudoviruses, you should clone the spike protein sequence into the envelope plasmid.
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I like the theory and how didactic the explanation is... But I find it hard to find videos about it showing the practical procedures. But thank you for this video.
Great video! If I wanted to knock out a gene in C. diff by using bacteriophage deployment of the cas9 protein, complementary gene sequence, and the guide rna, how would I do so?. A few more questions are: 1. Which of the 3 viral delivery methods would be bacteriophage? 2. How would I go about designing the dna strand that I want to insert? 3. How would I get the edited bacteriophage to grow clones? 4. How can I assure that the bacteriophage will target the C. diff? 5. How can I tell if c. diff uses the Nhej method or the hdr method of dna repair? 6. What can I use to find the base sequence of a particular gene in the c. diff genome? Sorry for all of the questions!! I hope that you all can help 😁
