So I started being crazy with slants -- I added an injection port to the top and now I pretty much use slants rather than dishes. Once I've isolated the genetics I like, into the slants they go -- and now that I have injection ports I can quickly add in 10cc of LC mix, do a bit of shake and extract if I want to inoculate a big mother jar. I may even play with making mini-LCs out of some of the spare tubes... much rather lose a 50ml tube to contam rather than a 600ml jar :)
Bravo! Bravo! Rah! Rah! Rah!!!! It is Pioneers like you that this "Renaissance of the Psyche & Health" needs my friend..!! First time in all of these Y.T. videos that I come across a brilliant idea instead of Ass kissers, Praisers & Whinny complainers... Well done! And for the 'material' used by one petri dish, you could inoculate 4-5 slants and come up with 4-5 more possible clean growths and one tenth of the risk for infections since the diameter of the slants is 10X smaller than the "mouth" of an regular agar dish! Then transfer your infection-free sample to a Really Clean Dish and Ump your rate of success by 500% Right? Let me know if 'this' idea compliments yours... Thanks NCShooter..! Are you from N. Carolina? I have a Sister there...
I forgot all the procedures I learned years ago in college. Your excellent instruction in this video has not only reminded me of the SOP but inspired my confidence to get back in to the lab. God bless you sir! SUBSCRIBED.
Same! Im about to have to turn myself in and do a little time and i wanted to make sure i keep all my best genetics for when i get home! Once again, Thanks PGT👊🏻🤘🏻
Dude... Stop kissing his arse like he is Moses or a prophet... The guy is great because he invested the time and effort doing what he does and now he's sharing and making money from RU-vid... He learned it the same way we all learned how to do anything. Give him a like and get off your knees or people are gonna start getting funny ideas... Jesus was a Legend.. And a myth... Phylly is just a Man...
Love your stuff man. Slants are "Outdated and Old-school".. Want long term storage without refrigeration? PC some tap water in a tube/jar/container etc. (yes, tap water, you can use distilled but you don't need it. the minerals are beneficial) for an hour at 15 psi. Take a chunk of your agar plate with your strain you wish to store and put it in the sterile water once cooled. (can put as much in as you want to store, but don't over pack it) Put a cap/lid/stopper on tight, and that's it... Done. Op. seal up the cap with cling wrap, parafilm, or grafting tape for extra protection if you want. It will stay good for 4-5+ years as is.. Slants are Out dated. I use 20 mill cryogenic tubes and have my entire library of 120 strains in a small container slightly larger than a big mac container, sitting on my lab bench.. I've come back six years later to one I forgot I had and it was just as fresh as the day I put it in there. Hope this helps anyone reading this.
@@droidnick The mycelium, remains on the agar. All your doing is taking chunks of your agar, just like you would do if you were inoculating grain jars with agar plate. only instead of a grain jar, you're placing it in a jar of plain old water. the mycelium remains in a dormmate state on the agar. when you go to use it, simply grab slice of the agar you placed in the jar and inoculate some grain with it. The mycelium will be on the agar within the water jar.
@billbill5396 This is brilliant if it works! Would you point me in the direction of any literature on this? Is there a Tek name for it? I can stretch LC out a couple years if I keep it very lean, but this sounds like a game changer.
@@droidnick it's not new and it absolutely works.. Edward Grand uses this same tech. I was told. He is a brilliant mycologist. And has a channel on RU-vid.. look him up, be ready to learn all things mycology.. hope this helps
@@droidnick I found out about it by accident actually. had a left over chunk and just threw it in a PC'd jar of water to use later. Forgot about it and found it years later and inoculated a grain jar with it. took off no problem. Unlike a LC you are not giving it any nutrient to grow, and it just stays dormmate even at room temp. Follow correct sterile procedure and you should have no problem. I recommend it for storage only, every time you open it up, you run the risk of contamination. I don't know if there is a name or not for it. But someone said Edward uses the same technique, and I know he has a channel and even does Q and A's.. So you can sub him and ask more about it if you like.
Enigmas are absolutely amazing had some of the most profound trips on enigma. I've had PE, golden teacher, mexicana, and possibly liberty 🗽 😅 but enigma had an amazing calming effect I recommend for micro dose changed my life 😀. But the true trips I quit 🚬 after 29yrs and all hard booze 😀 then I started micro dosing with them its amazing they sharpen your vision lesson any anxiety depression, and keep me calm, very respectful and confident in my purpose in life, and anything.!!
I know you have a lot on your plate philly and yet here you are gracing us with more knowledge to add to our arsenal. Remember philly, nothing is worth your sanity, make sure you prioritize you and don’t over extend yourself! We love you man! 🙏🍄❤️
Ethan... Cool your motherly love for the new baby... He gets RU-vid milk ($$) for what he does. It's nice that he does it & the only one risking its 'sanity' here is you.
Backing off on the lids allows pressure equalisation and stops the plastic parts of vessels from deforming in the heat under pressure of expanding air. Also, if you don't put water in your jars, the agar won't boil over, putting sugars on the threads of your lids, like what happened in your video, and potentially inviting contam. It's the hot steam that surrounds the vessels that produces the heat to sterilise everything over time, not so much the steam that enters vessels. Maybe steam getting into the slants helps a little bit, but it's not the primary reason we keep lids loose in an autoclave.
I understand that, general consensus on shroomery was to back up the lid 1/4 turn and seal with parafilm to allow gas exchange. Would you agree or not?
Good logical thinking! Unlike Mr. Wang the Tang above who wants the man to do another whole video to 'explain' how to transfer a bit of Mycelium from a petri dish to a 'slanted' petri dish... For an Asian guy, "he not too smart"..!
Too funny, You uploaded this video right as I was making some slants myself. I sadly don't have any cultures to put in them yet though. I'll have to go hunting for some funny fungi =)
Hey there bud! I love the videos and thank you for your content! Comment on the food colouring, I think 5 drops to such a small amount of liquid may have been too much. The blue "leaking" out of the jar may have just the high concentration of colouring allowing the dye itself to still want to travel and find media to colour if that makes sense. I am saying this cause you just taught me some thing and i would like to watch you continue with colours :D
Hey Philly,love your work brother, I've been going back and watching all of your vids to brush up on things and maybe learn something I missed,and I have had a lot of success from using your instructions and am interested in using slants eventually,haven't made it that far yet and having to take a break on things myself but I would like to see and maybe others will too your method of getting transfers out of a slant without messing things up I know it can't be much harder than taking a plate transfer but would still maybe make an interesting video if not I understand and hope you are having a good weekend.
Great video! Thank you. I have one question: can the thinner (corn dog) sticks work? For whatever reason i thought the 15ml tubes were going to be more narrow.
This agar recipe is different from your specified Corn Syrup-Potato Flake Recipe. Is it specific for slants? Do you prefer one recipe over the other? Situation dependent?
I just got a reishi slant and have no idea how to transfer a piece to agar . I’ve tried using a razor knife and could barely even get a piece out to transfer
Would there be any benefit to using activated charcoal instead of food coloring? I understand that activated charcoal might have an antibacterial effect.
I was initially confused about the extra nutrients in there, but a quick search on shroomery informed me that the agar soaks the wooden rod and makes it accessible to the colonizing mycelium. Is this correct?
The dye getting into the water would indicate that all the solution is getting in there. The dye wouldn’t separate itself, and without it you would never know. That said, does it make a difference if the solution comes out a bit? Is it boiling over?
Hey I have found a old bag of specimens is there any way to do a Lazarus with old dried fish concert , I read about tissue culture but a video would always be helpful
have you has any problems with the blue caps in the PC ? i have been told to only buy the green caps (thought i'd ask to find out some facts) great video, thanks
I don't see a difference here if you tape either of them with micro-pore tape or any other type... Even Saran Wrap cut into tape-form can seal anything...
I'm curious how your slants didn't melt. Is it because you put them in a glass jar first? Beginner here, but i put my agar plates in the PC, rather than trying to go from a beer bottle to the petri dish in a still air box. Well i opened the cooker to find melted dishes and agar water. I'm done wasting money. What slant tubes did you use?
The slants are a special plastic that can withstand pressure cooking…. Your melted plates means you ordered plastic plates probably “presterilized” in a plastic that doesnt withstand PCing. Polypropeleyne is one type of plastic that is autoclaveable/PCable
Is it normal for colored agar to start to lose its color within a month of swabbing your agar? Most of the surface space is colonized but it was red and now it's yellow.
Question, why not use the boiled water from hydrating the popsicle sticks for the agar? Wouldn't it make a more nutritious agar, especially for wood lovers?
Just keep making mistakes and keep looking for answers. Its a learning experience. PGT is probably one of the guys i mostly follow. Everyone has different procedures, you just have to find what works for you.
No, not really... Slants are dorms for the mycelium because they lack the sugar needed to grow faster. Slants= powder grain + agar. Agar plates: Same + Plus sugar in any form for energy and fast growth. That is why slants are only used for long-term storage... Not to grow mycelium. (still grows but at a slower rate..)
I personally don't see what the big deal is about wiping the colored water spew off the slant's plastic... It is not like it's hair dye or a permanent marker mess... The food dye is a mild agent that is easily diluted and dissolved in any media that is applied/added to, otherwise, if it was so bad, we'll all be walking around with different colored ass holes glowing in the dark ('cause the Sun don't shine there, right?) so, the convenience of having different colored cultures for easily identifying bacteria & mold vs not using any, I'd say is barely significant... Just infuse your paper towel sheet in Alcohol and you'll be disinfecting & cleaning at the same time... Gee...
On 90 second mycology’s channel he mentioned that a quarter inch is perfect because the mycelium only grow outwards, not up and down, so don’t worry that’s completely normal
Also I’m pretty sure that’s why he tilted them onto the side in this video, he said it’s to increase surface area, but if the mycelium grew up and down the surface area trick would be pointless
In my opinion, N.Y. is by far the best ingredient you can add to any agar mix... It boosts growth, even a pinch of it and you can use it in any combination mixes. You just have to super-duper sterilize your media since bacteria loves yeast..!! Try it.
Yes. The culture will have more food and take longer to go through the whole tube. That said, if you keep these smaller ones in the fridge they’ll probably hold out for a year already bc the cold slows the growth so much. We use 50ml tubes but mostly because the opening is larger and easier to work with
At 13:50 you mention "70% isopropyl...". You've mentioned this in several other videos as well. I've got 99% isopropyl alcohol, but had a vague recollection that 70% was actually more effective as a germ killer. I looked it up and yes: 70% isopropyl alcohol (and 30% water) is much more effective as a sterilizing agent. Rubbing alcohol is also 70%, but may contain oils, scents. Simplest is to get 99% and dilute accordingly I think.
Sorry for saying this but it does present barriers for the hard of hearing as the word translations do not always coincide to the actual wording being spoken. Why do people think they have to put any kind of music in the back ground It makes for an ugly video no matter what the content of the video is about. Questions; If you want the cultures to stay longer, why do you slant them, allowing only a small amount of nutrients at the bottom, I would think leaving them at a much higher angle would be much more beneficial for time storage Or if one was to use a jar with considerably more depth to the agar. Would that not be allowing the culture to live or stay longer, And would one be able to freeze them rather than just at fridge temperatures. I do realize there is less culture to chose from when vertical, But I would think if the culture is good, one should have no problem collect myc.
The myc doesn't penetrate that deep, the depth is mostly so it doesn't dry out like it would if it were just shallow the whole way. You want it to be a lot of surface area so the myc has lots of space to live, if you did it vertically it would be more likely that the myc dies.
