How to make Platelet Rich Plasma (PRP) with no Kit!

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How to make #PRP with no Kit!
Making platelet rich plasma going from from 120cc blood to 6cc PRP.
Draw Blood: Each 60cc syringe has 53cc blood and 7cc ACD-A
Split into two 50cc sized test tubes (30cc in each tube)
Centrifuge RCF = 2170
First spin = 2.5 min
After First Spin.....
Draw off only the yellow plasma/platelets with a blunt needle and place ALL of the plasma in one 50cc syringe (will be about 25cc -30cc plasma)
Add 1.5cc ACD-A to the same 50cc test tube.
Counter balance with the left over blood into a second 50cc test tube (about 30cc)
Second spin = 5.0 min
After Second Spin
Discard the counterbalance test tube.
Draw off the plasma until you get to the desired end volume.
Resuspend the platelets in the bottom back into the plasma.

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2 окт 2024

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Комментарии : 20

@texasorthobiologics Год назад

Platelet count (blood)= 214 ; Platelet count(PRP)= 2827. WBC(blood) =3.1 WBC (PRP) = 11.5 GRA(blood)=1.8 GRA(PRP)=1.4 LYM(blood)=1.00 LYM(PRP)= 8.50 MON (blood)=0.3 Mon (PRP) =1.6

@fuganetti 2 месяца назад

So 13.2x concentration factor and a platelet dose of 16.9 billion total in the 6ml? Impressive!

@donbufordmd 5 месяцев назад

You can check PRP in a hematology machine. You should first check the platelet range on your machine to make sure it covers platelet count to up to around 3500 or 4000 so you don’t have to dilute the PRP

@tonypatz9387 2 месяца назад

Do you add ACDA tin the drawing syringe? If so how much.

@marce005 Месяц назад

Any reply?

@shawnisabellalin8021 4 месяца назад

Do you need to activate with calcium?

@Dr-789 5 месяцев назад

Can we check platelets and other components in PRP by hematology machine that uses usually for CBC etc or it can damage hematology machine ? Thanx

@texasorthobiologics 2 месяца назад

yes you can and it will not damage the machine. For higher platelet concentrations you should check the range on your hematology machine to make sure it can handle platelet counts over 1000....and higher in the PRP ranges.

@donbufordmd 4 месяца назад

Activation is not ecesssry for PRP to work. PRP is activated pretty rapidly upon. Injection anyway

@satiashaw1857 2 месяца назад

Wow! So informative, thank you!!

@20573867291823047859 2 месяца назад

It seems to me it would simplify the process and collect more platelets by using a gel separator tube. After a single spin, you could aspirate the top 80-90% of the plasma. Then you could re-suspend the remaining plasma and platelets in the first tube (thanks to the separator). This should get nearly all of the platelets since you dont have to eyeball it, stopping at the RBC.

@texasorthobiologics 2 месяца назад

Tried...gel separator tubes typically have a very low platelet recovery percentage ...about 50% worse than the technique shown here. There are many reasons for this...but the easiest way to prove it is to run blood at the same time on the two techniques and get the data... we have done that.

@shawnisabellalin8021 4 месяца назад

You had acda in the blood draw, and you add acda again for the second spin?

@donbufordmd 4 месяца назад

Not every kit protocol does that....but yes, I do add 1.5cc of ACD-A into the test tube for the second spin to make sure there is no platelet aggregation. We have tested this and done it this way for 7 years or so.

@wreamdeaver8931 4 месяца назад

How long can the blood sit before spinning for plasma?

@texasorthobiologics 2 месяца назад

if you mean after drawing the blood....3-4 hours if handled properly

@Dr-789 5 месяцев назад

Good but u left a lot in first tube which is first PRP ...so my advise to you as dont discord too much in tube after first spin...

@donbufordmd 5 месяцев назад

Unfortunately that is simply not true when we actually test that supposition. After the first spin the red is nearly 100% RBC When making LR-PRP we don’t want any of that. After many thousands of doses and a few published papers on this and BMC, I think we have a pretty reliable way to do this. Thanks for taking the time to comment

@Dr-789 5 месяцев назад

@@donbufordmd U did not understand me...i am not saying ,not to do double spin...i am actually saying dont left a lot of plasma in tube after first spine as i seen the video...bcoz still there are a lot of platelets in bottom of plasma layer even after first spine which will yield better PRP after second spine....so dont discord plasma after first spin at bottom and take that precious plasma that is close to RBC layer ...thanx

@donbufordmd 5 месяцев назад

@@Dr-789 I understand, thanks for the clarification. The typical platelet recovery percentage with the technique shown is 80% which is good......When the goal is truly leukocyte poor I think 80% is good enough platelet recovery. The WBC in the protocol shown usually drops from baseline as opposed to a LR-PRP where I would get 1 or 2 cc of the "red" which would give more platelets and leukocytes also.