thank you for these videos. You mentioned in the videos that after preparing the plasma, you could cool it and administer it, or activate it and administer it. Does that mean that you do not necesarily have to activate it? thank you!
Whether you activate it or not will depend on your application. The platelets will be somewhat activated on injection by collagen in the tissue. If you want more activation in a localized area (tendon injections) then you would probably want to activate it before injection so that a fibrin clot will rapidly form and keep the platelets localized. If you want the PRP to spread and slowly activate (some cosmetic applications) then you would not want to activate it before injection.
The second syringe already contains concentrated PRP. There is no reason to spin it again. However, if you wanted even more concentrated PRP you could spin it again and then separate the layers once more. But of course if you do this you will get a reduced yield because of platelet loss during the process.
I think you may have misunderstood. We don't "add" any RBCs. We include the part of the buffy coat that is stained with RBCs because about half of the platelets are in that portion. Yes, this means we have some RBCs in the PRP. But without that portion our platelet yield would be much lower.
Most people use calcium. Either calcium gluconate or calcium chloride will work. We use 10%CaCl3 at a 1:20 ratio. You can also use thrombin or a host of other chemicals (platelets are very sensitive!) but we have no experience with these.
