The polysorbate 80 is a surfactant and helps the oil based fragrance blend in with the rest of the ingredients which are water based. Without it, the fragrance will separate from the mixture and the beads will not be able to soak it in. Hope this helped answer your question!
Thanks for the question! After testing the beads, their scent lasted about two weeks before we topped them off with more fragrance mixture to freshen them up.