idk what youtube algorithm is doing but its already far beyond human comprehension. I watched the entire thing even though i dont even own or use this stuff.
Why don't you? You should get them! You're missing out! Think of all the fluids you could pipette into vials! And then pipette them out of those vials into other vials! Tbh I reckon this should be an olympic sport. >_> I'm kidding, I'm kidding. But it can be useful for routine testing of things. Are you a gardener? Fancy hybridizing or cloning a specific plant to improve it or make it resilient to something? NPK measurements of hydrponic solution benefit from good lab technique like this, note it all down, and then perfect your mix!
@@bashkillszombiesHaha olympic gold medal for pipetting sounds kind of nice. But yes i agree, its useful to know some day. I have actually grown some oyster mushrooms once and that was quite hard since everything had to be sterile. I have grown some plants too. Who knows maybe some day i can use this.
1st of all why was this recommended to me? 2nd of all I am on vacation right now, I didn't really want to have anything to do with work related things. 3rd and last point. I use the pipette frickin everyday in the lab, for years by now, I should know how to use it. So tell me what has been going on in youtubes mind?????
Eppendorf tips are mostly universal except for certain manufacturers like Rainin but matching manufacturer tips with each pipette will produce the greatest accuracy and precision.
There's air between the liquid and the piston, so aspirating a few times lets surface molecules evaporate off the surface and saturate that air. This way you don't lose anything potentially significant to evaporation into that space. That's the explanation on ThermoFisher's site. Maybe it's most significant for very small volumes?
When dispensing the liquid does the pipette tip need to be against the wall of the test tube? And must the pipette be vertical position when dispensing the liquid? Thank you!
You have to pipette in the exact same way. If there is any residual it’s probably because it’s not liquid only but some of the cells or their debris might attach to the tip.
I wished she would've shown both ends of the pipette when measuring. It gives room for misinterpretation if you don't show both actions at the same time.
If the pipette tip is clean and attached from the original container and not immersed into the sample beyond the minimum necessary depth, there is no need to wipe the tip. Wiping only increases the opportunity of absorbing the sample from the tip and rendering the sample volume inaccurate.
You do not need to wipe the tip. Also with the small volumes you pipette with these at times it could greatly affect your results. But since these tips are not wiped you should pay attention to immerse your tip just enough for it to take up the liquid Pushing to the second pressure point allows all the liquid within the tip to come out. Normally wiping is only done with large glass pipettes and even then the tissue shouldn't touch the tip as to not suck out liquid accidentally
If the angle of immersion is not followed properly, there'll be inaccuracies in the amount of fluid withdrawn as well as drawing up of air bubbles especially if the fluid withdrawn is of low volume (for example, withdrawing 0.2ml from a 0.35ml sample.) If the angle of ejection is not 20-45° and in contact with the deposit vessel walls, there could also be erroneous delivery of such sample. Air bubbles are caused by different factors including poor immersion depth (the commonest), the type of fluid, using the wrong pipette tips, releasing the plunger too fast and some other factors that depends on the skills of the person using the micropipette.
@@p.m.satyanarayana8505 Choose the appropriate tip. When putting on the tip, apply a little pressure on the tip to make sure it fits perfectly to prevent any spaces around the borders of the tip and the micropipette. The pressure should be enough to make the tip stay on the pipette and also enough to make the tip fall off when it's time to eject the tip using the ejector slide on the side of the pipette.
@@p.m.satyanarayana8505 Then that's probably a mechanical problem. If I understand you well. You can use newer pipettes or send the said pipette for repair if the tip isn't gripping the pipette because the metal part is loose..
It actually also differs from manufacturer to manufacturer. And depends on the range of the pipette. Reference precision scales can be found in the relevant protocols on the companies website (look up your pipette model and then the calibration protocols). You'd still need to check your pipettes in regular intervals and send them in if your result is outside the referenced precision scale
Didn't know what this was and was curious - saw the beautiful lady with the sensational voice and decided to say......after 3:09mins I didn't know know what this was all about. Bye for now Dr. Henke.
