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how to transform E coli by heat shock 

Learning Planet Institute
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A synthetic biology mooc sponsored by Mairie de Paris, Fondation Liliane Bettencourt Schueller, Citizen Cyberlab FP7 produced by mooc factory CRI Paris.

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3 окт 2024

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Комментарии : 19   
@anglman3234
@anglman3234 5 лет назад
Very well explained. I like your presentation, very nice and simple without the burden of heavy jargons. You deserve an award for the best teacher
@raslalique
@raslalique 3 года назад
However, if you keep skipping the jargon you'll learn a lot slower in the long run.
@KJKP
@KJKP 3 года назад
I really enjoyed the instructional style of the presenter.
@santelmo5222
@santelmo5222 7 лет назад
Lights, cells, action!
@spectator59
@spectator59 2 года назад
Good content and style. However, is the sterile technique used here really good enough?
@duyduong8519
@duyduong8519 5 лет назад
Thank you! I learned interesting knowledge from you.
@daxtonjabari3088
@daxtonjabari3088 3 года назад
You probably dont give a damn but does any of you know of a method to get back into an instagram account..? I was stupid forgot the account password. I would love any help you can give me.
@camiloramon3477
@camiloramon3477 3 года назад
@Daxton Jabari instablaster :)
@daxtonjabari3088
@daxtonjabari3088 3 года назад
@Camilo Ramon thanks so much for your reply. I found the site thru google and I'm trying it out now. I see it takes a while so I will reply here later with my results.
@daxtonjabari3088
@daxtonjabari3088 3 года назад
@Camilo Ramon it worked and I actually got access to my account again. I'm so happy! Thanks so much, you really help me out!
@camiloramon3477
@camiloramon3477 3 года назад
@Daxton Jabari you are welcome :D
@LeonardoGomez-wb6jr
@LeonardoGomez-wb6jr Год назад
Is the LB media added with the antibiotic?
@kazmkucuk1663
@kazmkucuk1663 2 года назад
Cold environment during transformation maintain cell membrane movement stability. Thus increases chance of plasmid to migrate into cell.
@rajghosh4485
@rajghosh4485 2 года назад
How do we calculate the transformation efficiency by following this protocol?
@acrobacia
@acrobacia 3 года назад
MADRE MIA!!
@anuradhapandit9824
@anuradhapandit9824 7 лет назад
After aliquoting from the 200 ul competent cells, can you refreeze it? Does it effect the cells in anyway? Thanks....
@PRITAM0000
@PRITAM0000 4 года назад
খুব ভালো
@alukooluseun8661
@alukooluseun8661 4 года назад
please what are the beads for?
@KJKP
@KJKP 3 года назад
The beads behave as vectors to distribute the bacteria.
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