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İneklerde oosit toplama (collecting cow oocytes) 

ender yalcinkaya kalyan
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Просмотров 5 тыс.
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A brief description of how we collect oocytes from bovine ovaries. The first step of bovine IVF.

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10 сен 2024

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Комментарии : 31   
@papodecripto5062
@papodecripto5062 4 месяца назад
Parabéns
@mohdshamshad1932
@mohdshamshad1932 4 месяца назад
Thanks for sharing your valuable knowledge and experience, please can you send me the more information regarding the procedure ( step by step). Actually i want to use it in Goats.
@enderyalcinkayakalyan
@enderyalcinkayakalyan 4 месяца назад
Thanks for your attention. I have not been performing bovine oocyte ivm for a long time. For that reason, I dont have the protocol for now. I guess you can find a standard protocol on google very easily. I tried to show the technical part in this video. Good luck
@emiliebaker4010
@emiliebaker4010 5 месяцев назад
Thank you for this video! Say I am not interested in using the oocytes past grading them. Do i need to put them in a media to look under the scope?
@enderyalcinkayakalyan
@enderyalcinkayakalyan 5 месяцев назад
It will be easier if you put them into a small drop of culture media to check easily
@DrAli-jv9si
@DrAli-jv9si 3 года назад
Thanks for sharing your knowledge and experience.. You said the washing solution is pbs.. What is the medium that's have collected egg?
@enderyalcinkayakalyan
@enderyalcinkayakalyan 3 года назад
Thanks for your valuable comment on my video Dr Ali. In this experiment, I used a fertilization medium including Tyrode's medium as the basal salt solution and NaHCO3, PYRUVATE, LACTATE, gentamycin and BSA as the supplements. I can send you the formulation if you are interested via email.
@DrAli-jv9si
@DrAli-jv9si 3 года назад
@@enderyalcinkayakalyan thanks for your feedback to my comment I'll be glad if you email me for sharing more details please.. teşekkür ederim😊 dr.alialiraqi88@gmail.com
@santiagobidart9884
@santiagobidart9884 3 года назад
@@enderyalcinkayakalyan very interesting video, thanks for sharing knowledge. Mayy i have the formulation as dr ali please? my email is santiagobidart@hotmail.es Thank you!
@enderyalcinkayakalyan
@enderyalcinkayakalyan 3 года назад
@@santiagobidart9884 Already sent!
@elizabethraquelingamachuca8735
@elizabethraquelingamachuca8735 3 года назад
@@enderyalcinkayakalyan ... very interesting video, thanks for sharing knowledge. Can I have the formulation as dr ali please? my email is raquel3116@gmail.com, Thank you!
@ZNaim-ez8my
@ZNaim-ez8my 3 года назад
Which type of filter is used for oocyte collection? Plz reply. Thank you.
@enderyalcinkayakalyan
@enderyalcinkayakalyan 3 года назад
I used a Falcon cell strainer with 100 um pore size to remove big cells here.
@ZNaim-ez8my
@ZNaim-ez8my 3 года назад
You are incredible 😍 Can you please give me the step by step procedure of oocyte maturation?
@enderyalcinkayakalyan
@enderyalcinkayakalyan 3 года назад
Maybe I need to make another video on this;) Keep following me✌️
@amolcnikam
@amolcnikam 2 года назад
very nice ender !
@enderyalcinkayakalyan
@enderyalcinkayakalyan 2 года назад
🙏
@777hodhod
@777hodhod Год назад
can you tell me the name of this separation filter ( company name and its contact ) i need one
@enderyalcinkayakalyan
@enderyalcinkayakalyan Год назад
If you mean cell strainer it is corning brand. You should search for a distributor in your country. I dont have contact right now;sorry
@777hodhod
@777hodhod Год назад
@@enderyalcinkayakalyan I mean the filter on min 3:14 which separate the oocyte from the fluid and please can you send me your whats up to contact you iam ahmad from egypt
@enderyalcinkayakalyan
@enderyalcinkayakalyan Год назад
@@777hodhod It is a cell strainer which we also use for IVM in human. You can ask your questions via info@embenderyalcinkaya.com
@777hodhod
@777hodhod Год назад
@@enderyalcinkayakalyan which diameter is the best for bovine oocyte ? 40 or 70 or 100 um
@enderyalcinkayakalyan
@enderyalcinkayakalyan Год назад
@@777hodhod I used 100 but all is ok
@ihgaming6856
@ihgaming6856 4 года назад
Kindly use English language for understanding of different countries people. Thanks
@enderyalcinkayakalyan
@enderyalcinkayakalyan 4 года назад
Thanks for your interest. I will add the subtitles as soon as possible.
@dr.ashutoshbasera9210
@dr.ashutoshbasera9210 3 года назад
U should speak in english or give subtitle
@enderyalcinkayakalyan
@enderyalcinkayakalyan 3 года назад
The video has subtitles I guess;)
@souheylabenbia4889
@souheylabenbia4889 Год назад
i need your email please
@enderyalcinkayakalyan
@enderyalcinkayakalyan Год назад
enderyalcinkayakalyan@gmail.com
@souheylabenbia4889
@souheylabenbia4889 Год назад
@@enderyalcinkayakalyan thanks, i will contact you in challah
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