ID Laboratory Videos: Antibiotic susceptibility testing

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"Antibiotic susceptibility testing", 2015.
Produced by Tufts University School of Medicine Center for Science Education (CSE) as part of the Infectious Disease high school curriculum:
sites.tufts.edu/ctse/great-di....
The Great Diseases curriculum provides a framework for students to learn high school biology in a more approachable and life-relevant context, designed to improve science engagement and health literacy.
Supported by the National Institute of Allergy and Infectious Diseases (1R25AI098781-01A1).
Written by EmilyKate McDonough and Kayle Sawyer.
Filmed and edited by EmilyKate McDonough.
Actors: EmilyKate McDonough, Revati Masilamani, Berri Jacque, and Eleanor Fleming
Lab space and materials: Andrew Camilli, Katya Heldwein, Tufts CSE
Music: "Discomutante" - A Smile for Timbuctu (alterations made) and "Air Hockey Saloon" - Chris Zabriskie.
Animation consultant: Andy Musser

Опубликовано:

16 июн 2016

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Комментарии : 155

@TimidAction 3 года назад

for any poor soul who needs to find the 9 mistakes Labeled the top of the agar plate rather than the bottom. Did not keep the cotton tip of the swab sterile. Did not orient the swab correctly when selecting a single bacteria colony from the stock culture plate. Did not spread the bacteria colony evenly over the agar plate to create a lawn of growth. Did not discard the swab in the waste container. Moved the disc after it fell on the agar plate. Placed a disc too close to the edge of the plate so it was not possible to measure the diameter of the zone of inhibition. Dropped a disc on the benchtop. Did not space the discs evenly on the agar plate.

@Old_Guard2 3 года назад

Thanks from the future, you are a life-saver

@livybearful 3 года назад

I love you

@juandiegofajardohernandez5369 2 года назад

@@livybearful omg, thank you! HAHA

@afnansyifamuhammad9016 2 года назад

OMG, Thanks So Much

@vanessaestrela1050 2 года назад

You saved me!!! thank you so much!

@iqdefinition3253 4 года назад

Thanks for not rushing through but putting it in simple and understandable terms. And that yellow glove scientist I found humorous though important as these are mistakes we could make but now we are alerted to be careful. thank you again and please keep helping students like us.

@mitra2028 2 года назад

Are antibiotic resistance test diagnosed by doing Urine for C/s test?

@therubinlab Год назад

I like the idea of including food colouring, that's a great teaching trick to show the concentration gradient! I think my students sometimes struggle with visualizing this with a verbal explanation alone.

@nourserag5991 2 года назад

WOW the experiment now for me seems so easy after watching every step of it carefully and also the brief summary in the end made it even more illustratable to one's mind .thank you

@SavePaws_FriendlyWorld Год назад

Best educational Concept Clarity.... Ur nature of Guiding is Excellent 👌 Now I can Study, rather than boring my Nuisance lectures

@navaneeth_us 2 года назад

Top-notch conceptual correlation and beautifully crafted and video. Thank you so much.

@manaseetelaang328 2 года назад

This made the concept incredibly clear, thank you!!

@asifkhanrahime445 5 лет назад

I like your way of teaching please keep doing all these stuff about microbiology..... And if you have time please make some videos about its full details........... THANK YOU SO MUCH

@hassanalaa2112 3 года назад

do you know what are the 9 mistakes ??

@yogeshlakshman8488 2 года назад

You explained it like a movie mam It is so awesome hoping more videos Thank you so much❤️

@adrianamathai7481 5 лет назад

This was so helpful! Thank you!

@samassefa8579 3 года назад

Great video. Super clear explanation!

@SavePaws_FriendlyWorld Год назад

U r just amazing.... crystal clear Concept. Thanks a lot madam🥰 amazing video

@adammajaliwa3125 2 года назад

U 're so amazing madam 🙌 u make it short but very much clear and educative too 🙏🙏

@liketylickchick 4 года назад

Thank you for doing this video! I think you're an excellent teacher, also very pretty!

@manikankana2553 2 года назад

That's one engaging and informative video. Thank you.

@poosichomper 2 года назад

I'm learning this at school, For science and I find this very interesting!

@shalleey 4 года назад

Thank you!❤️

@RedRose-gw9bt Год назад

I absolutely love your channel, but what type of bacteria did you use for this lab?

@azizaabdumuminova3135 6 лет назад

Great and helpful video 👍

@sunandinisingh589 2 года назад

Thank you sooo much! I could not visualize this by reading from my book!

@prettysavage2203 5 месяцев назад

So beautifully addressed this lecture ❤😊

@shresthamukherjee6071 2 года назад

So helpful 💯 Thank you so much

@user-tj6rf5tr7r 3 года назад

Очень полезное видео, спасибо большое!

@chrissychris9967 5 лет назад

Thank you

@pauldradre7169 6 лет назад

Thank you for your video

@anisahmad2714 3 года назад

Thanks for the video!

@daryaissa3191 4 года назад

Thank u so helpful ❤️

@erickemanueli898 4 года назад

this was fabulous

@sankimseng6094 2 года назад

This so useful and help me a lot Thank!

@matthiaseckert7344 2 года назад

Very nicely done

@gabrielrogerio7128 3 года назад

muito obrigado!!

@aboodameri2145 5 лет назад

Thank you Soooo much

@malakilani6283 2 года назад

that was amazing, thank you!

@komlalebu8333 5 лет назад

thank you so much

@roselycullen9071 6 лет назад

Thank you it's quite interesting and helpful

@dr.umheart2388 3 месяца назад

Thank you so much

@ujumiriam9174 2 года назад

Educative, thanks for this

@aichamni3543 3 года назад

I'm just a biggenner and my English is not very good but I understand this video it was so helpful thanks a lot 😘😘

@kmyachan9795 2 года назад

Thank you very much

@naimanasrin7073 2 года назад

Thank you sooo much!!!!

@45bounty 3 года назад

perfect..thks so much

@moh19931000 3 года назад

Do we have to do this in a laminar flow hood?

@user-ch9ng3no9m 3 года назад

Thank you bae 😍

@abdixakiinmukhtarcmm116 2 года назад

I very interesting the way u explain thnkz

@asifkhanrahime445 5 лет назад

Thank u

@dayanacheong901 6 лет назад

what is the name for the antimicrobial disc with the code W 2.5 ?

@endymion_hyp 3 года назад

What dye was used to show the diffusion zone?

@user-cx5in7pi5c День назад

Heat, measurable scale, reference table, sterilization

@mltlecturesbyTT83 3 года назад

Excellent

@tozfkfkvhv6053 2 года назад

Good morning great information sister

@nurulhidayah-ls6rc 5 лет назад

Great and helpful.. thank you

@hassanalaa2112 3 года назад

do you know what are the 9 mistakes ??

@user-wz8he9lx8i Год назад

تشکر .مرسی.tankeyou

@zakariazaki7513 2 года назад

I like this video keep going 🤠 greeting from Morocco

@tatendachawanda6129 5 лет назад

Well Explained

@hassanalaa2112 3 года назад

do you know what are the 9 mistakes ??

@idrisadamu5133 5 месяцев назад

Very interesting.

@tomasescribanoselak4334 Год назад

Awesome!

@jericoramirez-ac5397 Год назад

What is likely to happen if antibiotic discs are placed on the inoculated media and left at room temperature for an hour before incubation?

@RedRose-gw9bt Год назад

I'm looking for a lab idea (fast) and I was wondering if E.coli bacteria was used for this lab?

@axlaamali7560 4 года назад

great d helpful thank you

@rafiaakhter2214 5 лет назад

what are the aseptic techniques that were used

@krmanjahmad659 4 года назад

thanks for this type of vedio i love it.

@hassanalaa2112 3 года назад

do you know what are the 9 mistakes ??

@krmanjahmad659 3 года назад

@@hassanalaa2112 @Hassan Alaa yea 1.labeling is wrong, with line writing. 2. contamination of swab. 3.wrong in striking of plate. 4. mistake in puting antibiotics in target place. 5. not handling of cup plate. 6. mistake in taking one colony. 7. forgeting small dot(.) on swab 8.upside down of plate that cause differentiation . 9. not adding another same antibiotic on plate. 10. not taking care of safety (like not putting swab in waste container).

@Peace-fo1vx 4 года назад

thanks a lot

@hassanalaa2112 3 года назад

do you know what are the 9 mistakes ??

@bilalahmad6102 5 лет назад

thnks ma'am.

@Keepstudying-ex1vw 5 лет назад

I have one quaries ,i have isolated endophytic bacteria from plant ,then pure culture ,and i want to test my isolated strain on pathogenic bacteria by MIC Method,but i dont know how to perform it.

@yuniarelfira5793 5 лет назад

thanks for the video. But, i want to ask something about the preparation of inoculum. I read the protocol of Kirby-Bauer Disk Diffusion Susceptibility and it said that the preparation mus be followed by 0.5 McFarland standard. It means we have to check the density of our inoculum according to 0.5 McFarland standard. On your video, you just pick one colony from the agar then spred it with the sterile swab. According to your opinion, which one is better for the preparation of inoculum? And how the differences between them? thank you

@divyanshumishra4802 5 лет назад

yeah...I am agree to you...

@innocentabae1118 Год назад

Beautiful 🥰🥰

@bangashkpk7914 6 лет назад

thanks madam

@divya821 2 года назад

Mam provide more like multiculture on different samples on single media

@aya.h8846 3 года назад

What about the 9 mistakes?

@aarani4655 3 года назад

Thank you so much, really helped with my homework! :)

@hassanalaa2112 3 года назад

do you know what are the 9 mistakes ??

@humeeayisha3199 5 лет назад

Good job 👏

@hassanalaa2112 3 года назад

do you know what are the 9 mistakes ??

@humeeayisha3199 3 года назад

@@hassanalaa2112 no please Tell me what they are

@user-ve8yt7dy6e 3 года назад

هذا شرح يخبل وشغل مفصل مو مثلنا كضينا كل أسبوع تأجل الشغل عالورا 🚶‍♂️💔

@velusamypothiraj2300 2 года назад

In result they mension "sensitive" what it is means

@shalugoutam1763 3 года назад

So nice

@hassanalaa2112 3 года назад

do you know what are the 9 mistakes ??

@MD-bf2ce 2 года назад

I'm very new to Microbiology, but just about flipped when yellow grabbed the sterile cotton tip by the tip! Ahh!

@nonyequeto8406 Год назад

Whats the method of inoculation? Thank u

@sam_khan_1995 5 лет назад

Thans

@nargizaraximova762 3 года назад

It is interesting Video thanks,😇

@buhariabdullahi3128 5 лет назад

Biochemical test

@Az.Beauty.by.C 3 года назад

what if a disc does not have a halo over it ? does it mean it is resistant to the antibiotic?

@pinkeupanda6265 3 года назад

yes

@naomiyorgba2420 4 года назад

Pls make more video

@jennie-jen8138 6 лет назад

Great video.

@hassanalaa2112 3 года назад

do you know what are the 9 mistakes ??

@sokefunful 2 года назад

Narrator: Label the bottom of the plate Yellow gloves: how about no...

@zeenatullah9960 4 года назад

Gr8 madom

@alakwahid 5 лет назад

Do you use tetracycline in different dosage for six time?? Or different antibiotics for six time?? Someone explain me

@drhumajawed7536 5 лет назад

She used 6 different antibiotic, but here we can use one antibiotic with six different concentration

@hassanalaa2112 3 года назад

@@drhumajawed7536 do you know what are the 9 mistakes ??

@jakechandler5702 3 года назад

Why is she hating on scientists who wear yellow gloves? -_-

@olduglyentwife5700 3 года назад

They have messy handwriting

@hassanalaa2112 3 года назад

do you know what are the 9 mistakes ??

@bauhiniafolia9673 3 года назад

Wait, how about the 9 mistakes?

@kimberlycalip7981 3 года назад

Same,

@sivaprasadaraoboppudi4804 4 года назад

procedure explain in english

@stellafuego 4 года назад

Hi! I'm just a newbie. Just wondering how can I use garlic or other natural antibiotics for antimicrobial susceptibility test? I hope someone would answer.

@mostafijurrahmanalhadi5292 4 года назад

You need to extract the juice from garlic or other natural sources, storing them in ethanol. Make a disc soaked with those extract at different conc. (e. g. 30/60/90 microL or as you want). After preparing the discs with those natural extract, simply follow this spreading method and put your discs on the plate. Search for papers or articles on this.

@stellafuego 4 года назад

@@mostafijurrahmanalhadi5292 thanksssss

@bsparks4211 4 года назад

I like your question , impressive

@FunnyMoments-qh6lx 3 года назад

@@mostafijurrahmanalhadi5292 Men I had the same question and you answer it perfectly

@oulythorng3922 2 года назад

How to prepare the antibiotic dics?

@khalafalasad3209 4 года назад

Nice

@hassanalaa2112 3 года назад

do you know what are the 9 mistakes ??

@dallonpihema16 6 лет назад

Thanks exam passed

@hassanalaa2112 3 года назад

do you know what are the 9 mistakes ??

@A000a97 3 года назад

@@hassanalaa2112 I found 6 mistake 1 It is mistake when picking the coloni 2 Contamination of swab cotton 3 false way to distribute 4 don't put swab cotton in recyclbin 5 antibiotic fall from forceps 6 growth of bacteria not true

@unknownuser8297 3 года назад

What are the 9 mistakes?

@moonlight950 3 года назад

I also want to know the 6 errors in the video

@BrittanyTeague12398 3 года назад

There are a total of 9 mistakes. If you actually watch the video, you will see the mistakes. She basically points them out for you.

@pigeonlove 3 года назад

Stooopid

@hassanalaa2112 3 года назад

@@BrittanyTeague12398 do you know what are the 9 mistakes ?? just say it briefly... p.s: i spoted 6 mistakes

@mustafarahi8670 Год назад

Ur mom

@zahraafakih7020 2 года назад

To grow the bacteria no meed for a specific medium right? Just wonderning

@heatherwelberry1 3 года назад

Why in the world would you tell us there would be 9 mistakes and then not tell us what they were so we could see if we got the right one s?? That's actually cruel, you know !!!

@kamalkaperera3599 Год назад

D diomand Test

@mikemiller7231 Год назад

Why are they so baffled at doctor offices when you ask for them to do a simple test like this? Compartmentalized idiots we usually call them now! We did this in high school. Now they get frustrated rude and say bs.