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Immuno PCR with IQELISA® [Why you may need it] 

RayBiotech
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More on the Immuno-PCR Kit platform (IQELISA®):
- www.raybiotech...
More on Services: www.raybiotech...
The IQELISA is an immuno-PCR assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR (RT-PCR, also referred to as quantitative PCR or qPCR), providing you with a detection platform that uses familiar ELISA workflows while pushing the frontiers of your detection limits and sample volume requirements.
The benefits of an IQELISA include over a standard ELISA include:
Up to 10x more sensitivity-As with all ELISAs, assay sensitivity depends on the antibodies' sensitivities. However, the lowest limits of quantification (LLOQs) of IQELISA are, on average, 23-fold lower than standard ELISA when using the same antigen-antibody pair.
Sample volume requirements as low as 10 µL-Compared to a standard ELISA, IQELISA immuno-PCR kits require 1/10th the sample volume, ensuring quantitative detection even when the sample is limiting.

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21 авг 2024

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Комментарии : 2   
@jayashreelaxmekuppuswami8600
How does it compare to western blotting????
@RayBiotech
@RayBiotech Год назад
Both ELISA and Western blot use antibodies to detect proteins. ELISAs require an antibody pair where both antibodies detect the target protein to provide a signal. A western separates proteins by size and uses one antibody to detect the protein and another antibody to detect that antibody. The ELISA and IQELISA technique uses a 96-well platform that is typically more user friendly and allows more sample throughput per experiment. Additionally, quantitative data can be obtained on a routine basis with ELISA and IQELISA whereas with the western blot it can be more demanding.
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