Increased NAD With Clover Sprouts 

Thanks @adamd9418, for the next 72+ years, until I (hopefully) break the longevity record!

@@conqueragingordietrying1797 Hi, I have a question do the Red clover is the same as Crimson and next question what about estrogen we know the plants increase

Thanks Olya!

Thanks @xscale!

Thanks @barrie888!

I'm currently on-track to double clover sprout intake relative to this test (250g vs 121g), so it will be interesting to see if it's a real effect!

@@conqueragingordietrying1797 how much does it cost per day to take clover sprouts?

Thanks @BZ4. The latest NAD results are in, though, for a 2x increase for clover sprouts, and NAD definitely didn't increase-video coming soon

Thanks @mervynman6303. I eat the majority of the strawberries frozen in a daily smoothie with ~330g raw beets and 60g fresh parsley, which are nutritional All-Stars.

@@conqueragingordietrying1797 Thanks. Strawberry season is coming.

Cool, thanks @jonathansoaresdonascimento4059!

Good idea!

Interesting, and thanks @the123apz. 3% trigonelline = 30 mg/gram capsule. That would be 4 capsules for this recent test to yield 120 mg trigonelline, but 8 to equate the trigonelline content coming mostly from clover sprouts for the next. In other words, a lot of pills to match what I can get from diet...

Truth ​@@conqueragingordietrying1797

Definitely James. Plasma levels have been increasing over the past 2 tests with the inclusion of trigonelline-containing foods, with minor effects on biomarkers, though (so far)

Thanks @gravityhaha. I always try to optimize biomarkers via diet as a primary strategy, so if I can do it without nicotinic acid, that's the goal Low-dose NA may aggravate my GI tract in a similar way to tryptophan-I'm correspondingly doing that experiment by taking it out here and there while testing NAD levels...

Thanks @berdi4berdi4. Current clover sprout intake is 250g/d (2x the amount in the video), so it's a real effect, I'm expecting another NAD increase for Monday's test...

Hi Ed, and thanks. They might be yellow for a day or 2, but switch to green around day 3 or 4 btw, recent NAD results are in with 2x clover sprouts, and NAD definitely didn't increase. There may be a U-shaped curve for trigonelline, I'm not yet sure...

@@conqueragingordietrying1797 , I am putting dried parsley on almost everything I eat as another way to keep my NAD+ up as I age.

he did try 600 mg Niacin and reached the highest NAD levels with 67.4

@@MrSuninlove Niacin is the best one, the other ones have no evidence over niacin and are more "unnatural" making them likely less effective

@@MrSuninlove no he didn't He tried Nicotinamide (NM) 600mg That's totally different from naicn

He tried nicotinic acid 600mg. ​@@xjoy3920

Hey Peter, my plasma trigonelline level increased to > 1uM (1.1, 1.7) for the last 2 tests (previous 5 test avg: 0.3uM) in conjunction with the small NAD bump for the latest test. I wonder if your NAD would also be ~30uM with a similar plasma trigonelline level, but other NAD precursors can impact NAD levels, too, so also maybe not.

This is a question that befuddles me as well, also in conjunction with the research on other coffee health benefits - in which case it mostly is about the beverage. However, I don't particularly like the beverage, but can eat some roasted beans - which raises the question about the correct dosage (I get jittery from a much smaller weight of beans vs weight of grounds in a cup) and whether the same health effects apply, given the differing preparation.

@@conqueragingordietrying1797 Haven't tested NAD but will. Focused on other markers I need to optimize better.

@@LVArturs I drink 1 cup caffeinated and two cups decaf. Don't think I'll risk beans straight. As for being jittery I think if you take L-theanine along with caffeine you might avoid.

I'd like to increase NAD without supplements, as a primary approach...

@@conqueragingordietrying1797 Nichola Conlon has a couple good interviews on Siim Land's channel if you'd like to get a taste for her thinking about boosting NAD. The ingredients in her supplement are just posted out in the open on her website if you want to experiment with them individually or research them individually. (e.g. she likes nicotinamide over nicotinic acid).

Thanks @michael-qp9xd. I'm not sure about how the seeds taste-I'm more than happy to sprout them instead...

I wasn't aware, thanks @AT-xl9db. I'm not worried about estrogenic effects-total testosterone was in the 750-970 range for the past 3 tests, so even if clover sprouts make a dent on that, I have plenty of testosterone room to be ok

I'd bet likely yes, but at what dose? Or, how many seeds would have to boil to get enough trigonelline in the water to impact NAD?

Niacinamide does raise NAD, but it's been reported to increase homocysteine more than nicotinic acid, which is why I've focused on the latter, not former.

@@conqueragingordietrying1797 If homocysteine is such an issue why not just get the boost from a more holistic approach in the body with silymarin or selenium or (glycine+NAC) all which boosts glutathione/SOD/NAD and so on and call it a day with the niacin compound supplementing craze?

Thanks @solverapproved! True Leaf Market in the US, unfortunately I'm not sure if it's available internationally

red clover is Trifolium Incarnatum. ...and that is a unique name. It is pretty cheap (if available), here it costs 8€ per kilo, and sprouts are clean

@@monnoo8221 Note that I'm growing them myself, which I didn't mention in the video

Hi @lucacavalli4963, Crown prince, boneless, in olive oil

@@conqueragingordietrying1797 thank you!

There's published data that blood levels can increase in conjunction with muscle levels: pubmed.ncbi.nlm.nih.gov/32386566/ We'd need muscle biopsy to confirm, which is impractical (and I don't want to donate muscle to prove a point). To try to get around that, metabolomics (iollo's kit) can provide insight about what may be happening inside tissues, so I'm sending blood for NAD and metabolomic analysis on the same testing day

Hi @justinmireau9150, I already did that experiment, no luck: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE--xOSWlyrAb0.html

No NAD increase for chickpeas: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-KHiImuSKf34.html

Hi @Bamse747, I have, but with limited success in terms of homocysteine so far

A major focus of the channel is to not use supplements (only if necessary), and to go after diet first

Yep, I did that experiment, no luck with increasing NAD: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE--xOSWlyrAb0.html

@@conqueragingordietrying1797 so that would mean that trigonelline could not be responsible for increase in NAD levels.

@@cookmeadrinkshow4814 It's a dose question-clover seeds likely have a lot more trigonelline vs commercially available fenugreek seeds.

Yes, thanks, but the focus of the video is attempting to increase NAD via diet, not supplements

Nicotinic acid is a potential confounder when testing the clover sprouts-NAD relationship (if it exists). I fixed that for the next test, removing nicotinic acid. Thanks for the suggestion!

Thanks @soez_strg6166!

Hi @HopeItStixStudios, the lowest hanging fruit (in my case) is leanness, as the majority of biomarkers have improved with a 20 lb weight loss. I don't know your body composition, but that could be a good place to start...

@@conqueragingordietrying1797 no I have a healthy BMI. 130 at 5”’6’. Where do I go from there

Hi Paul, and thanks! I got the clover seeds from True Leaf Market, and then sprouted them myself. That's potentially bad news f they're sold out... I'm in Boston, not NY

@@conqueragingordietrying1797 Well, which variety of clover from Ture Leaf are you buying? I'm on the site right now, and there are Mamouth Red and Medium Red Clover seeds available in pounds. But the Organic Red Clover is only available in small pouches. Hmmm, that's the same situation with Johnny's Seeds, too. See what you did?! You posted this video and sold out the entire organic red clover seed bank in North America! You're a Boston guy? No accent. You really seem like a NYC area guy to me. I liked the point you made in the last video with Chris about this VO2 Max protocol only giving us a chance to live to mid-80's, maybe. The Bible says Moses lived to 150! And I guarantee Moses wasn't pumping iron or training for any marathons. My grandmother sat by her 3rd story window in the Bronx smoking Marlboro Red after Marlboro Red to the tune of 4-packs a day and lived to be a healthy 94. But, she survived colon cancer in her late 20's and ate almost nothing every day. I guess if you wear a colostomy bag eating anything probably doesn't seem ideal. The only thing my grandmother ate was cold cuts from the deli on her block. And even with that she'd maybe eat a few slices of roast beef and swiss cheese. The only vegetable I ever saw here eat was a few spoonfuls of potato salad. I think that CR (Calorie Restriction) is a powerful tool. I've been watching Peter Attia for 10 years and He gets carried away with his exercise regimen when it comes to the average person. I'm a certified Health Coach, not charging anyone at this point, and getting people to just add steps to their day or take up walking is asking a lot. Thinking people are going to stat pumping iron and worrying about VO2 max def isn't for the general population. I also get pissed at his....what I think...is over the top protein recommendations. He Don Layman on---who has connections to the cattle industry---and Don Layman is like, "Eat two cows and call me in the morning" when it comes to daily protein intake. It's too much! Attia made that minny clip where he says "I can find no evidence that high protein intake correlates with a higher risk of cancer". That's probably not verbatim, but Jeez Peter I can easily find a direct correlation between high protein intake and cancer in reports written by NIH. Anyway....hope you find the clover seeds. Its super cool you are growing your own sprouts. If you haven't grown microgreens yet be careful its addictive. Plus, the leafy greens anyone on the East Coast buys from a grocery store are best case scenario already 4-5 days old. It's actually better buying frozen. You lose the fiber but you get more vitamins. Later...

@@paulfiedler9128 Crimson Clover - Organic - Sprouting Seeds Ha, I'm originally from Brooklyn, not Boston

Ok, why?

@@conqueragingordietrying1797 Perhaps it gets metabolized or used up in some pathway while the seeds sprout. On the other hand, during coffee bean maturity, the contents of trigonelline changes very little with coffee seed development, so it might be the same with clover sprouts. BTW, Trigonelline is present in green coffee at 1% dry weight, so this could be another source. During the roasting process of coffee beans, trigonelline changes into N-methylpyridinium and nicotinic acid as its major products, which makes it a useful index of the degree of roasting. Normal samples of roasted coffee contain from 95 to 263 μg./g. of nicotinic acid. The nicotinic acid content of coffee is not dependent on the variety of bean. So you may ant to check its effects on your NAD levels.

There is published data showing that blood NAD levels are similarly increased relative to muscle when NAD levels are restored with niacin: pubmed.ncbi.nlm.nih.gov/32386566/ Outside of a muscle biopsy, intra-tissue levels are impossible, but to try to get closer to the truth, I'm sending blood for plasma metabolomic analysis on the same day as every NAD test, which can provide insight in terms of what be happening in tissues.