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Inoculating Grain to Grain - Making Mushroom Spawn 

PhillyGoldenTeacher
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Quick lesson today on grain to grain transfers.
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30 янв 2021

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Комментарии : 330   
@davidafenderson3446
@davidafenderson3446 3 года назад
After watching your videos for the past month I have successfully created my own: SAB Spore syringes (totally sterile) Agar plates done with your solo cup idea Growing spores on agar (totally sterile) Isolating my first genetics Successfully developed my own unique recipe of grain I have a shelf full of about 30 jars with mycelium running like Bolt Spawned three shoebox tubs and it all looks great. Thank you sensei, I really appreciate you taking the time to collect all this information and show us, step by step, how easy it is. Once I have extra funds I’m going to support the Patreon. 🍄💛
@morghan_glamour8214
@morghan_glamour8214 3 года назад
Do you know what technique he used to colonize the first jar???
@Enochulate88
@Enochulate88 2 года назад
Good job fam
@timothywallace5507
@timothywallace5507 2 года назад
@@morghan_glamour8214 agar to to bird seed
@0psec_not_good
@0psec_not_good Год назад
Good stuff man! Keep at it!
@Gingerbred_Hed
@Gingerbred_Hed Год назад
Its because of you and the rookie mycologist that gave me confidence to grow my own medicine. Youre doing gods work. Keep it up!
@PhillyGoldenTeacher
@PhillyGoldenTeacher Год назад
🙏🍄
@darkangel686868
@darkangel686868 3 года назад
PGT, you make it look so simple, you’re a true pro!
@calebisenor6292
@calebisenor6292 3 года назад
It doesn't have to be complicated. The simpler the better.
@craig265
@craig265 3 года назад
I'm a pro ... But you will have to ask for me on broadway Friday night...
@shawnwillis767
@shawnwillis767 2 года назад
It is simple!!
@chandracox6814
@chandracox6814 Год назад
It is simple.
@zeezaweth6088
@zeezaweth6088 3 года назад
One main critique: Do ALL your shaking OUTSIDE of the box. Get your primary spawn jar broken up then place into box. Once you add the spawn to the next jars, just tighten the lid and set aside. As always, keep movement to a minimum inside the SAB. Once you have done all the jars, pull them from the SAB and spread that spawn. One thing I love about starting this hobby is there are hundreds of way to go about things. I am always learning and trying to dial in my own tek. Thanks again for another great video!
@markl4287
@markl4287 3 года назад
Do you crack lid a 1/4 turn to allow some gas in?
@donuts_are_good
@donuts_are_good Год назад
yup
@thorny3218
@thorny3218 10 месяцев назад
He working with a laminar flow 🤣
@philosophyman
@philosophyman 3 года назад
This is THE most important video for bulk
@wheatonna
@wheatonna 2 года назад
It's crazy how your 90% colonized jar looks so much less colonized after the shake-shake.
@VladTheImpalerTepesIII
@VladTheImpalerTepesIII Год назад
That's because technically it was only 20% colonized if that. A fully colonized jar of grain cannot be shaken loose, you have to go at it with a spoon (sterile) and work at scooping it out. If you can shake it loose and see grain, it's not ready yet for G2G and certainly not ready for spawn to bulk.
@LarryBird_
@LarryBird_ Месяц назад
@@VladTheImpalerTepesIIIthis is not true 😂 If you cant shake your grain loose before S2B you have bad spawn
@mmpatriot2170
@mmpatriot2170 Год назад
So simple. I didn’t even know this was possible. Thanks!
@frotob3816
@frotob3816 2 года назад
i’ve never had so much fun learning something. your videos make the flood of various contradictory information seem very much more digestible considering i have this baseline to refer to, giving me the confidence to start and learn more as i continue this journey. Thank you!
@jamesdubois2129
@jamesdubois2129 3 года назад
Im going to send my support when everything comes to fruition. Love your videos man very well put together
@smokenjoe001
@smokenjoe001 Год назад
No sir, THANK YOU for all your wonderful helpful videos and instructions. You truly are incredibly good at teaching.
@levihinsen1917
@levihinsen1917 3 года назад
Best content out there Philly🔥
@chadwalston28
@chadwalston28 2 года назад
Literally the best my dude. You give me confidence humanity will make it.
@EarthPoweredHippie
@EarthPoweredHippie Год назад
Thanks for everything bro, ur channel is so useful and well explained, as long as u put out these videos for the community I'll be here to learn.
@PhillyGoldenTeacher
@PhillyGoldenTeacher Год назад
Thank you! I wouldn't be able to do this without you and everyones support in this wonderful community. I'm glad you are a part of this journey! 😁
@joeyonelove4708
@joeyonelove4708 3 года назад
Thanks for the content PGT! Always look forward to a new video from the sensei! Damn look at that patreon list grow man! Come a long way since what 2 or 4 names on that jawn. Keep on keepin on brother!
@Kalashnikov762x39AK
@Kalashnikov762x39AK 3 года назад
Definitely gonna try this for my next inoculation ! Thanks for the info!
@barryspodge6972
@barryspodge6972 Год назад
Another straight to the point video thank you
@breathinginsilence
@breathinginsilence 3 года назад
love a good surprise video!!
@budlight9308
@budlight9308 3 года назад
perfect timing, thanks for great information, mush appreciated 🍄✌️
@Loumoli954
@Loumoli954 3 года назад
Thank you for sharing your knowledge!
@mr.b9127
@mr.b9127 3 года назад
Thanks PGT!
@chronomeatbag4223
@chronomeatbag4223 3 года назад
iv found its worth adding a few layers of 1-3mm vermiculite in LC grain jars if you dont shake during colonization you end up with so many tiny inoculation points when you use it for g2g.
@sage5757
@sage5757 3 года назад
Loving you're video's there helping my new hobby so much thank you sir🤘😛
@jay7282
@jay7282 3 года назад
Works great turned 3 half pints into 7 quarts for me
@cybergimp
@cybergimp 2 года назад
awesome! i have exactly 3 half pint jars colonizing and was wondering how far i could stretch them.
@MisterBones223
@MisterBones223 3 года назад
Excellent work! I'm a recent subscriber, and I'm fascinated with mycology. Thanks for giving us this information!
@tonyman1030
@tonyman1030 3 года назад
Froggo doggo? More like Froggo Druggo 💪🏻😎🍄
@connerwatson5818
@connerwatson5818 3 года назад
couldn't get to sleep and woke up to this beauty, keep up the awesomeness!
@mikehoncho2149
@mikehoncho2149 3 года назад
Same
@donkulasmushroomsandagri3123
@donkulasmushroomsandagri3123 2 года назад
Sir PGT 👍🍄
@tylerliberty4162
@tylerliberty4162 3 года назад
Thanks for these videos 🙏
@raulduke1528
@raulduke1528 Год назад
Hey your my favorite teacher.
@PhillyGoldenTeacher
@PhillyGoldenTeacher Год назад
Hey you're an amazing part of the community! 😁
@Mohammadparvazi
@Mohammadparvazi 11 месяцев назад
Awesome this was actually a great lesson. I learned a lot from you and this is my second day of leaving my bulk substrate and spawn in a monotub to give me mushrooms. Thanks
@PhillyGoldenTeacher
@PhillyGoldenTeacher 11 месяцев назад
I'm glad it helped! I wish you the best on your journey 🙏 🍄
@mitchelllott
@mitchelllott 3 месяца назад
big thx phillyG!
@benreed4918
@benreed4918 3 года назад
I did bird seed to Rye grain got full Colonisation I a week
@bigpimpin6085
@bigpimpin6085 3 года назад
1:47 god damn that was a smooth transition
@l0lan00b3
@l0lan00b3 3 года назад
Literally had me thinking he pulled another jar from the side lolol
@digdug6515
@digdug6515 3 года назад
TY Philly ☮️💕🍄
@shadyshrooms9056
@shadyshrooms9056 3 года назад
Respect Sensei
@williamwesley5078
@williamwesley5078 3 года назад
Great, my friend just did a g2g and its working! Do you count it as a 2nd Generation Mycelium because of its Maturaty. And is it a good way to Isolate a Specific Strain? This is my friends new fav hobby, he said it's quite easy once you know what your doing. Hes on his first bulk grow, thx for knowledge
@AngelAnge1
@AngelAnge1 Год назад
Amazing so easy! Thank you ❤️
@PhillyGoldenTeacher
@PhillyGoldenTeacher Год назад
Good luck on your journey! 😄
@jakemertz2660
@jakemertz2660 Год назад
Wow this gives me so much to think about!
@PhillyGoldenTeacher
@PhillyGoldenTeacher Год назад
😁 Learning is fun
@forestbracht5278
@forestbracht5278 2 года назад
Very helpful!
@metaglypto
@metaglypto 3 года назад
Newbie mushroom grower here. I have played around a bit with those mushroom blocks you buy to grow mushrooms. I was wondering if anyone has experimented with using those blocks after one or two fruitings, as an inoculant for another batch of mushrooms. I was thinking of using a 5 gallon bucket, with holes, of fresh substrate and an old "log" crumbled up for an inoculant. Anyone tried this, and if so, how did it work?
@KidCharlema9ne
@KidCharlema9ne 2 года назад
Hey PGT, have you had any increase in contam rates with those unmodded lids? How do you take them out of PC - Do you wait for them to cool before opening PC so contamned air can't get sucked in before you screw the top? I made some screw top no pours and was stunned when they triched out in the few minutes they were a quarter turn loose when I pulled from PC.
@MrCowboyjeff
@MrCowboyjeff 2 года назад
My question is that I'm about to transfer a Uncle Bens Rice bag to 7 Qt. jars of Oats. My Oats expanded a lot more than I was hoping. Would it be a bad idea to spoon out a couple inches of the oats before I add the rice? I am working in a SAB.
@lucacavalieri2735
@lucacavalieri2735 2 года назад
Hello Professor! Due to money and space, and having a grain spawn purchased online, I would go for a grain to grain transfer using 1,5 liters plastic bottles, do you think this could work? Thanks for your knowlede, always inspiring!
@khari83637
@khari83637 2 года назад
I have UB bag that has colonized grain on top that I want to use for g2g. assuming i use a SAB and sanitize properly - can I transfer uncolonized rice from a freshly opened UB bag to a grain jar with GE lid and spawn from jar instead of using the manufacturers bag?
@irjeco
@irjeco 3 года назад
Sensei! I love your vids this is my first time growing I have one question though, I inoculated two bags on the same day and kept them next to each other and one of them is doing so good already spreading mycelium and the other one looks like is doing some sort of oxygen exchange and its holding a stable humidity but I'm not seeing anything at all. Is this normal?
@tommyguntommy9816
@tommyguntommy9816 6 месяцев назад
Is there a way to extend the shelf life of a “donator” jar of inoculated grain? Such as in a way where this donor material can sit for months and be used from time to time without the mycelium expiring from lack of nutrition. Can this be achieved by a fridge? Or adding food from time to time? Thanks for any thoughts on this
@dillonjones1767
@dillonjones1767 2 года назад
Can this create an endless supply of mycelium? Once the 2nd one is fully developed spread that into new jars. Or will the process stop at some point?
@frankdatank2304
@frankdatank2304 3 года назад
So If I have a fast colonizing mycellium will it still be fast after grain to grain? Also would that be considered a genetic trait?
@kkrollingskkrollings3173
@kkrollingskkrollings3173 3 года назад
At this point do you leave the jars cracked a little to allow some oxygen in, i have watched most of your videos and i just found u and subbed, i think u said in a earlier video to keep open lid a liitle like a quarter turn or something but i dont remember for sure, as i have a bad memeroy. Thanks .
@kingmetroplex
@kingmetroplex Год назад
Brilliant
@gregoriotoscani9964
@gregoriotoscani9964 3 года назад
When you works with agar and jars you use a Still air box or a Laminar flow hood?
@darreno7602
@darreno7602 2 года назад
Would grain to grain transfer work if your colonized jar is essentially 90% colonized or is too far along to do so? I have an extra jar that wasn't ready at the time I moved the other jars to the sub. it has solid myc growth covering the entire jar and only a few grains are left to colonize
@entityknownaszlap7298
@entityknownaszlap7298 3 года назад
God man you have such great thumbnails
@bigtank222g4
@bigtank222g4 3 года назад
PGT can you do a class on isolating to monoculture. Having spotty harvest. Genetics suck. Thanks brother
@douglasmolik8069
@douglasmolik8069 3 года назад
Been there, keep @ it brother
@devinshilightsdale9738
@devinshilightsdale9738 3 года назад
Why do fully colonized jars look so uncolonized when you break it up?
@overzone666
@overzone666 2 года назад
Mycelium is microscopic :D
@spewdog
@spewdog Год назад
I think the mycellium bruises and changes color when it is broken up and turns white again after a few days. Maybe PhillyGoldenTeacher can elaborate
@stephenodubhlaoich
@stephenodubhlaoich Год назад
​@@spewdog Nah it's just because it's so fluffy, like cotton candy. A thick fully colonized agar plate will become just a piece of material around the size of a grain of rice or smaller on your finger if you swipe your finger around the whole thing. But it leaves small amounts of mycelium everywhere it already was before the shake and after it had already eaten from the grain so it regenerates easier.
@VladTheImpalerTepesIII
@VladTheImpalerTepesIII Год назад
Because it's not actually fully colonized ;). A fully colonized jar of grain cannot be shaken loose, you have to go at it with a spoon (sterile) and work at scooping it out. If you can shake it loose and see grain, it's not ready yet for G2G and certainly not ready for spawn to bulk.
@stephenodubhlaoich
@stephenodubhlaoich Год назад
@@VladTheImpalerTepesIII That's not true though. Mycelium grows outward every direction if it isn't on a 2d surface like agar. If the jar looks fully colonized from the outside of the jar, it's good on the inside too, for a transfer at least. A jar doesn't need to be consolidated so long that it refuses to break up, to be considered fully colonized. Neither me or anyone I know, including professional growers, let it get to that point before G2G. Although I do let them sit a few days or so after fully colonizing, to consolidate, before spawning. There's a difference between the two. Colonize just means when it's covered the grain, consolidate is when it has more time to digest it, and it gets thicker during this time. Also pouring grain for G2G is better than a spoon, so shaking it is something you should be able to do. But if you're not waiting a while for your jars to consolidate like that, and they're still hard to break up, it could be a sign of bacteria, which normally will still fruit, but not to it's potential.
@emrecelik4683
@emrecelik4683 5 месяцев назад
Should the jars be totaly sealed or should they be little bit open? I forgot to drill holes in my. Thanks for answer
@yung_dev1365
@yung_dev1365 6 месяцев назад
I didn’t take any grain from my jars before transferring to bulk would I be able to use mycelium from the tub for a transfer to grain jars or should I just take mycelium from the fruits after harvest?
@axelnio7498
@axelnio7498 2 года назад
Could i also use the content of my fruiting cake to colonize a new jar?
@SacredBear777
@SacredBear777 3 года назад
do you use manure for psyllicibin...or will coco work for it too and can i make this up a day in advancc before i inject spores
@Hernand3zFam
@Hernand3zFam 2 года назад
I'm new to mushrooms and I wanted to know could u use beans like pintos or another to use as my grain instead???
@MikeWMiller
@MikeWMiller 3 года назад
Always love the videos and the quality. Just one question -- is the wire cooling rack you always use in your SAB actually doing anything? Assuming you wipe down the bottom of your SAB as well as the sides when you begin, it seems like it does nothing except introduce instability to whatever you set on it...?
@PhillyGoldenTeacher
@PhillyGoldenTeacher 3 года назад
it provides an elevated surface to work on, and in my case it does provide stability because im using the lid of the SAB as the base and its flimsy and not really a true flat surface.
@ripme6616
@ripme6616 3 года назад
Mad!!
@Shady-bp6es
@Shady-bp6es 2 года назад
After a a jar fully colonizes can you place it in the fridge to store until ready to use?
@kembotmt
@kembotmt 3 года назад
PGT, Do you have any ideas of how I might maintain the room temperature once my substrate & grain go into my monotubs? My home sits at about 16 degrees Celsius (60F) throughout most of the day and night. Will that negatively affect my growth? Or just slow it down a little?
@nicinightbird
@nicinightbird 2 года назад
I had mine abort in the cold. I just got a lil heater from walmart :)
@muckimuck733
@muckimuck733 3 года назад
No holes in the led needed? Can I do G2G without a still airbox? Thx
@Jonnygurudesigns
@Jonnygurudesigns Год назад
How long can I store a grain spawn jar in a dark closet space? What are the options for a longer term storage ? A few months? Any ody have an idea?
@RyanWalsh83
@RyanWalsh83 2 года назад
How many times can you do it? Like, if I used one of the new grain to grain to create more. Then a new one from that to create more?
@terrymccorry8849
@terrymccorry8849 3 года назад
Ty
@BBJamesB
@BBJamesB 3 года назад
You said you can go grain to any other sort of grain, what about dirt to grain? Or dirt to dirt? I'm actually unaware if my jars are grain since I ordered them from Midwest Grow Kits. Have 12 jars inoculated but the site doesn't say that it is grain (or anything really). As long as the substrate is sterile, it shouldn't matter? Or am I wrong here...
@chrisd3372
@chrisd3372 3 года назад
Do the jars your transferring to need a hole for air?
@gep7175
@gep7175 3 года назад
When you are having your grain colonize in jars, do you need to have any air exchange holes with polyfill or filter patches? Or can it stay completely sealed. I know a self seal injection port is good for liquid culture for injections.
@dressedanddepressed2301
@dressedanddepressed2301 3 года назад
You can technically get by without any holes or modifications on the lid but growth may/can stall. I would leave the lids loosened 1/4th turn and flipped upside down if you aren’t using any holes or mods. If you use mods then use the micropore d surgical paper tape or polyfill
@cvictor8999
@cvictor8999 3 года назад
looks to me every jar is hermetically shut hence no air exchange of any kind. mycelium growth does not need oxygen to happen?
@afinedayforfinearts9670
@afinedayforfinearts9670 3 года назад
Can you do a video on reusing substrate?
@Allaboutpie
@Allaboutpie Год назад
Does anyone know if you can put grain in the bottom of your bag and your substrate on top. Wondering if an all in one bag would work. Just to basically only have to have a bag open once time to reduce chances of contamination.
@CindyyyToon
@CindyyyToon 2 года назад
This is great! I have one question though. Can I use any type of jar for this? And do the lids need to have breathing holes? The ones you used were fully sealed so I thought I should ask
@frotob3816
@frotob3816 2 года назад
any clean jar will work. he keeps the jars loose a quarter turn for air :-)
@ivan3465
@ivan3465 2 года назад
Thx❤️
@sa3kes111
@sa3kes111 11 месяцев назад
What if your grain is fully colonised and the myc is to strong to break and shake, how would you do G2G then?
@chessjohns6396
@chessjohns6396 2 года назад
PGT.im a noobie. are you doing this inside a sab? and since there are no ae holes in your lids once you transfer grain and shake it around do you then loosen lid a quarter turn ? thanx.
@richardview1281
@richardview1281 3 года назад
Love your videos. Do those plastic jar lids work just as well as the metal ones? My metal ones keep rusting and will switch over if it's all the same.
@PhillyGoldenTeacher
@PhillyGoldenTeacher 3 года назад
Yes I made a video about lids, check it out on my channel
@richardview1281
@richardview1281 3 года назад
@@PhillyGoldenTeacher thanks, will do
@rosevaldez8820
@rosevaldez8820 9 месяцев назад
Do you have syringe inoculation video ❤️ from syringes ? What’s are YOUR basics ?
@sirbloodyrectum
@sirbloodyrectum 3 года назад
How do you grain and grain transfer with brf tel???
@666grizwold
@666grizwold 2 года назад
Why many times when you are working in air still box you put your jars on this unstable metal crate? Is there some special reason for those crates? I'm anxious when watching that :D
@alexravelo6522
@alexravelo6522 3 года назад
Can you do the same process with substrate?
@Pandawea
@Pandawea 3 года назад
What kind of jars are those? How do You sterilize the grain un those?
@intergalacticprophylactic
@intergalacticprophylactic 3 года назад
ALL HAIL!
@redmoose7227
@redmoose7227 3 года назад
can you use the remnants of a basic growkit for this?
@knifeskillz911
@knifeskillz911 Месяц назад
Do you have to pressure cook the fresh grain jars your gunna use?
@witnessforchrist7778
@witnessforchrist7778 3 года назад
Can you keep doing grain to grain indefinitely? Thinking of it as sort of like a sourdough starter where you can continuously add to it and multiply it
@julian-io5wl
@julian-io5wl 3 года назад
Technically you can but it makes sense to start from time to time from agar to makes sure that your culture is not contaminated.
@spewdog
@spewdog Год назад
PhillyGoldenTeacher, does the mycellium change color when you break it up?
@sindelm7437
@sindelm7437 2 года назад
I have a butt ton of milo (sorghum). Wonder how 100% sorghum or an oat/sorghum mix will do 🤔
@judykonopka9029
@judykonopka9029 2 года назад
How long does mycelium stay viable in a grain jar?
@roostnboostmtb5013
@roostnboostmtb5013 3 года назад
How many times can you transfer to new jars?
@timm8311
@timm8311 3 года назад
Can you reuse one of the new 10 jars for another 10 jars, or is there a limit? Love the videos as I'm new to the delicatessen.
@lilbigGerm
@lilbigGerm Год назад
i’m a year late but yes you can!
@samigirardet6245
@samigirardet6245 Год назад
Hi, I'm wondering about this unmodified lid grain jars. There is no air exchange, doesn't that slow the colonization a lot? I had very slow growth when I don't have a good air exchange. What is your experience?
@PhillyGoldenTeacher
@PhillyGoldenTeacher Год назад
It can slow down some extent of growth potentially, but not to a large extent. It sort of depends on your need and wants for preference if you are really aiming towards maximizing production times, but it is not a major difference in most sense.
@pgdenik3890
@pgdenik3890 Год назад
Can i inoculate grain from already fruiting grow kit?
@njsurfnskate20
@njsurfnskate20 3 года назад
Love that wire rack to work on, is there a link you can provide? Thanks!
@PhillyGoldenTeacher
@PhillyGoldenTeacher 3 года назад
They're called baking racks amzn.to/36zCLGm
@richmac6345
@richmac6345 2 года назад
Is it essential to do this in front of a laminar flow hood? Great video. Thank you
@laurencerosenthalwinckler4439
2 options: plastic box with 2 glove holes (cheap option with low space) or flow hood with H14 filter. You can try your luck soon after shower in a toilet without carpets that u just sanitized like a OCD mom spraying alcohol on all the walls with all the windows and doors of the house closed using a mouth mask. Out of those 3 options its just too risky to get contaminations.. Like.. Those spores are literally EVERYWHERE.
@Lordskeep
@Lordskeep Год назад
Why do your jars have no holes in the lids? I've been told you need a hole for gas exchange. I'll go this method if you don't need any hole.
@Itswiggs93
@Itswiggs93 2 года назад
PGT, can you make a video on popcorn tek!?
@cruellust
@cruellust 2 года назад
Someone told me that the mycelium gets weaker each time you transfer is this true or false ?
@sjewitt22
@sjewitt22 2 года назад
can you put plastic jars in a pressure cooker?
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