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Introduction to spatial sequencing data analysis 

Sanbomics
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I show how to find, load, and process 10x genomics visium data. But I also explain how to load in spatial data from other platforms. This is useful if you want to analyze your own spatial-seq data, or supplement your project with analysis from already published data. I do this in python using scanpy.
Notebook:
github.com/mou...
Reference:
Murthy et al., Nature 2022
www.nature.com...

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20 сен 2024

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Комментарии : 38   
@梁一鹏-v7n
@梁一鹏-v7n 2 года назад
Your videos have been particularly helpful and I sincerely hope that you will consider doing a full video on the project of single cell rna analysis, similar to reproducing the Bioinformatic diagrams of some of the articles, which could help people to really understand how to interpret the actual problem. I take the liberty of bothering you and sincerely hope that more and more people subscribe to your channel
@sanbomics
@sanbomics 2 года назад
The reason I didn't have a video this week is because I decided to make a full single-cell rnaseq analysis video! Its at like 1+ hours so far and growing haha. Hopefully people will actually watch it! I'm doing almost exactly that. I found an interesting paper and I am reproducing some of the figures/analyses
@梁一鹏-v7n
@梁一鹏-v7n 2 года назад
@@sanbomics wow! I can’t wait to see it !!!!
@sanbomics
@sanbomics 2 года назад
I'm glad someone is! I'm a little worried that people wont click on a two hour video haha. But.... I'm gonna go for it xD
@梁一鹏-v7n
@梁一鹏-v7n 2 года назад
@@sanbomics Haha, I believe your 2 hour video can really help people a lot , thanks for your effort again
@sanbomics
@sanbomics 2 года назад
I managed to keep it to only 1 hour 18 min xD ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-uvyG9yLuNSE.html
@shreyaslabhsetwar6083
@shreyaslabhsetwar6083 Год назад
Amazing video! Awaiting your video on SquidPy!
@sanbomics
@sanbomics Год назад
By the time i do it there may be something even better xD
@shreyaslabhsetwar6083
@shreyaslabhsetwar6083 Год назад
@@sanbomics ahaha yeah the field is evolving soo rapidly!
@lessthanagamer1739
@lessthanagamer1739 2 месяца назад
Would really love to see a video on 10x genomics Xenium data processing as an aspiring high school researcher!
@libox8106
@libox8106 Год назад
Thanks for your time and effort.
@sanbomics
@sanbomics Год назад
Happy to help!
@urmumification1
@urmumification1 Год назад
Something which will be nice is an analysis between sections. Let’s say one spatial image before and after drug treatment
@yaseminsucu416
@yaseminsucu416 3 месяца назад
Hello! I am curious if you will have a much expanded version of the Spatial Seq Data analysis, also curious if there is any Proteomic analysis tutorial coming up! Thanks for your great work!
@sanbomics
@sanbomics 3 месяца назад
I might do a visium HD and/or a xenium tutorial soon but i have a lot of things in the queue and not enough time xD
@tamaterinha
@tamaterinha 3 месяца назад
@@sanbomics Visium HD would be amazing! I´m new to spatial transcriptomics and my first project is on visium hd data, am desperately looking for a nice workflow of analysis
@MrRamaeri
@MrRamaeri Год назад
thank you, it is very useful
@sanbomics
@sanbomics Год назад
You are welcome!
@neurostudywithme
@neurostudywithme Год назад
Can you make a video about spatial seq analysis on RStudio?
@sanbomics
@sanbomics Год назад
Unfortunately, unless there is a new tool that is specific to R that I want to try, I may not :(
@sriyellampally2649
@sriyellampally2649 Месяц назад
could you do a tutorial for geomx analysis
@vimalaa8721
@vimalaa8721 10 месяцев назад
Thanks for the great video. Could you please tell me how to convert a spatial seurat object into a scanpy for an integrated seurat object (two images)? Also, how should I handle these images in scanpy?
@Santiago-op3sb
@Santiago-op3sb 5 месяцев назад
Thank so much, I am performing Merfish (vizgen) analysis. Do you know when do we integrate all the samples toguether? Before normalization? after normalization of each sample but before PCA? thanksss
@ashwaqkhaled7530
@ashwaqkhaled7530 Год назад
Great video! How to annotate the clusters or the cell types?
@sanbomics
@sanbomics Год назад
I would say you can do it similarly to typical single-cell data. But just be aware that each spot is likely 5-20 cells. There are deconvolution methods you can look into as well that are supposed to break those into the individual cell types. Of course no deconvolution will be 100% biologically accurate though
@user-gl9kt4cf6n
@user-gl9kt4cf6n 10 месяцев назад
@Question: have done 10x spatial transcriptomics, and downstream analysis i try to use R, but the R generated UMAP (dim=30) is different from the UMAP that I se from the same data in the Loupe browser. I have played wtih the dim= 5, 10, 20, 30, still different? Any comment would be helpful. bthw: nice tutorial
@nepalchirag116
@nepalchirag116 Год назад
@Sanbomics Have you tried with Spatial-seq data using stereopy It would be great to got through, if you have a tutorial.
@sanbomics
@sanbomics Год назад
I haven't, but I will keep this in mind if i ever do!
@davidstivenarboledaprado8731
@davidstivenarboledaprado8731 3 месяца назад
Do you have a code for doing the same in R studio, I've been trying to built a Seurat object with public available data, using the counts, position and images, with no success. Thanks
@sanbomics
@sanbomics 3 месяца назад
Sorry :( only code for this in python atm. What step specifically are you having trouble with? The initial loading of the data?
@neurostudywithme
@neurostudywithme Год назад
Also can you make a video which you explain all the elements of a seurat object
@sanbomics
@sanbomics Год назад
I've thought about it. chatGPT might do a really good job at it!
@bioinfo3
@bioinfo3 Год назад
This is documented online in plenty of places
@bioinfo3
@bioinfo3 Год назад
Did you get an opportunity to do a follow up video where you demultipex each spot and use the squidpy package?
@sanbomics
@sanbomics Год назад
Not yet! I fear that by the time I do they will already have true single-cell spatial resolution xD (well there are some ways to do it already but its not mainstream yet)
@bioinfo3
@bioinfo3 Год назад
@@sanbomics what ways can you do it already? do you mean different technologies and not the Visium?
@sanbomics
@sanbomics Год назад
Yeah there are different ways than Visium. Even 10x has a new method coming out with higher resolution. They just aren't mainstream commercialized yet
@bioinfo3
@bioinfo3 Год назад
@@sanbomics What is the current best method to demultiplex each Visium spot or analyse 2 portions of the same Visium image? cheers
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