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IR Spectroscopy and Mass Spectrometry: Crash Course Organic Chemistry #5 

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It’s time for molecular analysis! On this episode of Crash Course Organic Chemistry, we’re learning about mass spectrometry and infrared spectroscopy through the lens of a forensic investigation. Put on your lab coats, and let’s solve this mystery!
Episode Sources:
Silverstein, R. M., Webster, F. X., Kiemle, D. J., & Bryce, D. L (2007). Spectrometric identification of organic compounds, 8th ed., Hoboken, NJ: John Wiley & Sons, Inc.
Spectra for pseudoephedrine: BioRad/Sadtler Data Collection, Bio-Rad Laboratories, Philadelphia, PA (US), Spectra ID: BR125952
Spectra for methamphetamine: Kunalan, V., Daeid, N. N, Kerr, W. J., Buchanan, H. A. S., McPherson, A. R., “Characterization of Route Specific Impurities Found in Methamphetamine Synthesized by the Leuckart and Reductive Amination Methods,” Anal Chem, 2009, 81, 7342-7348.
Other spectra reproduced with permission from: SDBSWeb : sdbs.db.aist.go.jp/ (National Institute of Advanced Industrial Science and Technology, 6/9/2020.
Series Sources:
Brown, W. H., Iverson, B. L., Ansyln, E. V., Foote, C., Organic Chemistry; 8th ed.; Cengage Learning, Boston, 2018.
Bruice, P. Y., Organic Chemistry, 7th ed.; Pearson Education, Inc., United States, 2014.
Clayden, J., Greeves, N., Warren., S., Organic Chemistry, 2nd ed.; Oxford University Press, New York, 2012.
Jones Jr., M.; Fleming, S. A., Organic Chemistry, 5th ed.; W. W. Norton & Company, New York, 2014.
Klein., D., Organic Chemistry; 1st ed.; John Wiley & Sons, United States, 2012.
Louden M., Organic Chemistry; 5th ed.; Roberts and Company Publishers, Colorado, 2009.
McMurry, J., Organic Chemistry, 9th ed.; Cengage Learning, Boston, 2016.
Smith, J. G., Organic chemistry; 6th ed.; McGraw-Hill Education, New York, 2020.
Wade., L. G., Organic Chemistry; 8th ed.; Pearson Education, Inc., United States, 2013.
***
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8 июн 2020

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Комментарии : 148   
@joymae
@joymae 4 года назад
I’m digging the purple accents in your backdrop and how it matches your sweater
@user-xx7pg3vw9k
@user-xx7pg3vw9k 4 года назад
Joy Mae that was no accident...
@animeandstuff5377
@animeandstuff5377 4 года назад
My condolences to those who didn’t get to watch this series while in organic chemistry 😔
@Laaaa
@Laaaa 4 года назад
Anime and Stuff this series is about 12 years too late for me 😩
@dogzrzosmz
@dogzrzosmz 4 года назад
I feel this deeply
@saumyamathur4862
@saumyamathur4862 4 года назад
I took it this same time last year, & while I’m happy that today’s ochem students have this at their fingertips, I’m also very jealous that I didn’t 😩😂
@kevinconrad6156
@kevinconrad6156 4 года назад
Condolences accepted. My lecture teacher was good when he stayed on topic which he did not do often. The lab teacher was great and often gave short lectures on what the lecture teacher missed.
@birzhanabdikhan8175
@birzhanabdikhan8175 4 года назад
Totally agree brother
@MrShipp100
@MrShipp100 4 года назад
My PhD is in advanced IR spectroscopy using femtosecond lasers, and this is a really nice concise explanation of the basics!
@definty
@definty 4 года назад
Isn't that just a laser that turns on and off very fast? Or is it something different?
@alarcon99
@alarcon99 4 года назад
@Shippers I stan
@extremdeath1234
@extremdeath1234 4 года назад
@@definty spectroscopy on the femtosecond time scale is a bit more than "very fast". It is ludicrously fast... you can actually monitor reaction on that time scale. for perspective, it takes about 300fs for molecular iodine to vibrate once...
@user-ik6wc1rn4j
@user-ik6wc1rn4j 4 года назад
WAIt , u are saying this is the BASIC?
@grad3us
@grad3us 4 года назад
Please, I want CrashCourse Neurology! It would be really cool to understand the brain, just as it was cool to understand computer science.
@glennng7612
@glennng7612 4 года назад
As a chem major, I really love how concise the explanations are. Great job crash course!
@xxstargirlxx
@xxstargirlxx 4 года назад
I saw Abby(from NCIS) So cute
@anne12876
@anne12876 4 года назад
Side note, during my first internship as chemistry student, I worked as a lab technician at the anti-doping centre for sports. I spent a summer analysing urine samples by IR spectrometry and sometimes by MS to check for illicite drugs. I was fun to see what happens in the lab during big big sports events and the science behind it.
@ariame2722
@ariame2722 4 года назад
The molecules dancing made my day 😭
@EverythingScience
@EverythingScience 4 года назад
I gotta say these videos seem much more advanced than the normal topics you guys cover
@MysteryMan1910
@MysteryMan1910 4 года назад
Just finished my PhD in structural mass spec. This is a pretty good intro! Although, at least in my field, no one really uses the magnetic sector MS instruments shown here anymore - we more often uses ToF, ion trap or orbitrap mass spectrometers.
@mawatake
@mawatake 4 года назад
I'm a Japanese, but I understand this content because of English subtitle ! I want to watch this series about 1H-NMR and 13C-NMR
@TheTwick
@TheTwick 4 года назад
I’ve always enjoyed that scene in a movie when a grizzled old detective runs his fingers through a black substance at a scene and says to the young detective “Send this back to the lab and have it analyzed.”!
@zagisch4789
@zagisch4789 4 года назад
I’m writing a home exam about the use of analytical chemistry in archaeology atm, so the timing of this is perfect 😍
@TheTravelerww
@TheTravelerww 4 года назад
the T.V scientist in the graphic; is that meant to be Abby Sciuto from NCIS, great reference.
@RedwoodGeorge
@RedwoodGeorge 4 года назад
A long time ago I worked for a company that made Fourier Transform Infrared Spectrometers. All new employees went through the two week customer training course to learn how the machines worked. In our class one of the customers was an evidence tech from the Royal Canadian Mounted Police - I was sitting next to an actual Mountie! Cool! One of the attachments for the spectrometer was a microscope - the Mountie had brought along a sample paint chip with three layers of different color paint. We focused the microscope on each layer and ran the results through the database to identify the brand, color and year of each layer! For even deeper analysis, we worked with a GC / Mass Spec to split up the sample by boiling point and molecular weight. The boiled off results were deposited on a cold plate in a labyrinth pattern and then the spectrometer sampled along the path to further analyze the components. Those were fun days working with some pretty cool machinery :-)
@christianandrews7764
@christianandrews7764 4 года назад
Was not expecting this but I’m liking the progression of this series! Looking forward to episode 6
@CrossingDS
@CrossingDS 4 года назад
As I biochemist, I found it very complete but also clear. Also, loved the effort put into the actual video!
@radunicoara8057
@radunicoara8057 4 года назад
I have finally learned what mass spectroscopy is. As a physics major, I never really understood it. Incredibly good course!
@BenTajer89
@BenTajer89 4 года назад
As somebody who's already been through highschool and undergrad O-Chem, I really wish your explanation of the mass spec had gone just a TINY bit more in depth, you describe all the parts and how the ion goes through, but none of how the magnet actually separates the masses as they go around the curve. This concept is actually super intuitive.
@user-pr1st5rh9r
@user-pr1st5rh9r Год назад
I just love this channel .. their way of teaching along with case studies, various examples is just so awesome & practical.❤️👍🏻Keep it up guys !
@emilyensing2184
@emilyensing2184 4 года назад
I love the major mass spec and abby reference in cartoon form!
@knate44
@knate44 4 года назад
Omg. Can't wait for the inevitable Crash Course Analytical chemistry!
@sheepyboi4219
@sheepyboi4219 4 года назад
Omg omg thankk youuuu. I have my Chemistry exam next week and this is something that is the hardest for me in unit one thanks so much!!
@farhanmendel
@farhanmendel 4 года назад
Crash Course about different analytical chemistry techniques and methods would be cool!
@foodaddict17
@foodaddict17 4 года назад
Love love love this! :D Explained so clearly
@timr8431
@timr8431 4 года назад
I have a chem degree and this even helped me understand it a little better. Great video
@kai_taylor6550
@kai_taylor6550 4 года назад
Love this i really needed to know this and its a great example thanks Crash Course Crew!!
@michelleelizabeth9956
@michelleelizabeth9956 Год назад
Wonderful videos and beautiful explanations , best of all. Thank you for sharing.
@Darfanatior
@Darfanatior 4 года назад
Crash Course is the coolest!
@bakryabdelmonem2066
@bakryabdelmonem2066 4 года назад
Amazing you making me love chemistry even more
@TriplaHHH
@TriplaHHH 4 года назад
Awesome :D Analytics gets often disregarded in chemistry-pop due to its complex backdrop. So thanks for the effort to lay this out somehow! Disregarding the instrumentation and datahandling though, the basic concepts of sample prep, separation and measurement in analysis are quite straightforward. Sounds like we are heading towards chromatography ;)
@enobnala90
@enobnala90 4 года назад
This is the only part of Organic Chemistry that I able to absorb!
@sofbsilva
@sofbsilva 4 года назад
You're about 5 days late. I had an exam about this on friday...
@bidishadey3815
@bidishadey3815 4 года назад
🤣🤣🤣
@UgoTemple
@UgoTemple 4 года назад
Organic Chemistry is one of my favorite
@Karajorma
@Karajorma 4 года назад
@vitali Mizrachi You're telling him what is his favourite?
@johnstevens1020
@johnstevens1020 4 года назад
YAYYY Another video from crash course!!!!!
@kevinfleming9918
@kevinfleming9918 4 года назад
Great explanation!
@RJ-nr8lh
@RJ-nr8lh 4 года назад
PLEASE BRING BACK CRASH COURSE LITERATURE. PLEASE WE LOVED THAT.
@Ogiwon
@Ogiwon 4 года назад
looks great! :)
@Veronica-wz8ow
@Veronica-wz8ow 4 года назад
I love you guys
@saurra3953
@saurra3953 4 года назад
Yay I was just reading this from Clayden!
@LucyRockprincess
@LucyRockprincess Год назад
excellent video
@geovannamartins2243
@geovannamartins2243 4 года назад
Great video! Greetings from Brazil!
@thebloxxer22
@thebloxxer22 4 года назад
You referenced Abby from NCIS in the beginning.
@VintageBlacklist
@VintageBlacklist 4 года назад
Abby is the best.
@pamelaevangelista434
@pamelaevangelista434 7 месяцев назад
Im an ib chem student and lemme tell you. Youre doing gods work, youre a miracle sent down from heaven, A NATIONAL TREASON!!! So thank you🥹, i might actually pass this class💀😵
@nicholasticali4623
@nicholasticali4623 4 года назад
This stuff is really awesome and was really fun, but it also brings back really really bad memories 😂
@enitmarin6805
@enitmarin6805 4 года назад
Genial job.
@xpalin1980
@xpalin1980 4 года назад
ABBY! I love abby from NISC she’s so cute :3
@finexjoshy9651
@finexjoshy9651 4 года назад
The new app does not load and when the first screen had loaded it showed that there was some error. Then I tried again and it still did not load even after 20 minutes
@sceptre1067
@sceptre1067 4 года назад
the ongoing use of purple triggers my youth watching Burke’s Connections in a good way. i feel old. 🤪
@atomicxfox
@atomicxfox 4 года назад
please make an in-depth video on IR and proton NMR graph interpretation!
@dangriff12
@dangriff12 4 года назад
If you have any specific questions just message me.
@honeybeewarrior5731
@honeybeewarrior5731 4 года назад
Incredible science
@techgirl4977
@techgirl4977 4 года назад
am i the only one who thinks crash course should be subscribed to by everyone in the world
@pyritium
@pyritium 4 месяца назад
What does DFTBA in the dialogue bubble over Deboki in the intro animation stand for?
@grahamrankin4725
@grahamrankin4725 4 года назад
Ephedrine and pseudoephedrine will have almost identical MS and IR because they are diastereoisomers. Both can be used to make meth.
@PavanKumar-xv1hg
@PavanKumar-xv1hg 4 года назад
thank you ?
@omsingharjit
@omsingharjit 4 года назад
Awesome Brilliant great
@zarahasan331
@zarahasan331 4 года назад
lol I have a lab final for this tomorrow... wish me luck.
@michaelmayhem350
@michaelmayhem350 4 года назад
You should make an hours log video of you just reading from textsbooks for us to listen to (no video necessary but it's never a bad thing)
@itstotallyezra
@itstotallyezra 4 года назад
We use spectrometry in archaeology !!!!!!!
@Synthetica9
@Synthetica9 4 года назад
Is that tucos compound from bb?
@KillerTacos54
@KillerTacos54 4 года назад
Our SAC is next week haha. Thank you for going over all this stuff. I think what would've been really helpful would be going through the different reaction pathways as well as H-NMR and C-NMR (because that's in our SAC). Thankfully, IR Spectroscopy is in it and mass spec was going to be in it, but it got taken out as well as chirality and geometric isomers. This stuff was taken out of our study design (syllabus) because of COVID-19 and everything. Btw if anyone else does VCE in Victoria, Australia: hi :). After that, we're moving onto chromatography like HPLC, paper chromatography and stuff
@dangriff12
@dangriff12 4 года назад
I need one on 2D NMR. It just looks like chicken pox and I have no idea what I am looking at. Any C13 NMR or DEPT 135 is fine though just message me any specifics.
@ZalyQQ
@ZalyQQ 4 года назад
I hope you teach HPLC before I need to use it 6 weeks :/
@kacibjordan
@kacibjordan 4 года назад
That’s Abby from NCIS!!
@dutchik5107
@dutchik5107 4 года назад
I had an exam on this last week. I barely passed. Could've used it earlier 😓
@andeexists8716
@andeexists8716 4 года назад
I wish I had these videos during A Level Chemistry lmaoooo
@Ft888x
@Ft888x 4 года назад
Same!
@amjadilham4174
@amjadilham4174 4 года назад
now i realize purple is actuallly dope
@henryelicker2403
@henryelicker2403 4 года назад
I'm getting mixed signals from this video.
@jeffreypomeroy6173
@jeffreypomeroy6173 4 года назад
So how do you tell the difference between chiral molecules?
@TriplaHHH
@TriplaHHH 4 года назад
Other techniques, like chiral column chromatography (i.e. pre-separation of chiral components, then detection with mass spec), or Raman spectroscopy (infrared-zone laser excitation. Raman optical activity experimentation is quite new, so I might make a mistake here). According to Wikipedia, there's also a possibility to apply kinetic resolution (i.e. react the isolated rasemic (both chiralities) mixture with a catalyst differentiated by the reaction rate.
@papachoudhary5482
@papachoudhary5482 4 года назад
Thanks
@jv4779
@jv4779 4 года назад
How do these processes deal with impure samples?
@ImmieHands
@ImmieHands 4 года назад
I'm currently working on my PhD in mass spec so probably alright to answer this. There are a couple of things we do to deal with impurities, firstly couple MS with some form of chromatography like GC or LC which separates out different compounds in a mixture based on their relative affinity for either the stationary or mobile phase, meaning different compounds enter the mass spec at different times. Along with that, if whatever you're studying is in a particularly dirty matrix, for instance I work with wastewater samples, you can perform various extraction techniques to remove the chemicals of interest from the sample matrix, leaving behind the impurities. Hope this helps!
@zarinawillows2347
@zarinawillows2347 4 года назад
13:16 VERY SMALL Mystery ?!?!!? You call that small....
@Greenmachine305
@Greenmachine305 4 года назад
It was a meth lab explosion. Got it.
@sofia.eris.bauhaus
@sofia.eris.bauhaus 4 года назад
no it was a pseudoephedrine lab smh
@danieljohnson3024
@danieljohnson3024 4 года назад
Someone had sinus congestion and couldn't smell the gas from when the pilot light went out.
@maksimghyvoronsky5641
@maksimghyvoronsky5641 4 года назад
sofia eris bauhaus You know pseudoephedrine is like one of the main ingredients in meth right? And since it was a LAB then yeah... what Bryan C. said
@antonyandrewson5803
@antonyandrewson5803 4 года назад
where was this when I was failing my a levels :'(
@kasualstudio7141
@kasualstudio7141 4 года назад
Those 16 dislikes are Hollywood producers
@dangriff12
@dangriff12 4 года назад
30 minutes is a long time to get a spectra! Even after running a blank to clean out the mass spec ours takes 3.5 minutes per run. So 7 minutes for the first spectra and 3.5 minutes for each subsequent spectra. It's not like the machine was super expensive either I think $4K.
@samiebelal8700
@samiebelal8700 4 года назад
Dude I just finished ochem dangit
@katiefinch9414
@katiefinch9414 4 года назад
Boy I hope Starburns made it out ok
@mohamedgamaleldeen4315
@mohamedgamaleldeen4315 4 года назад
How do I review the approach of data structures and algorithms before I study software engineering course Thanks
@hogo21
@hogo21 4 года назад
Ngl, I watched this just cause I saw Abby. Video is great though!!!
@Adenybaloi
@Adenybaloi 4 года назад
Yo, it's Abby
@Felixkeeg
@Felixkeeg 4 года назад
NMR best spectroscopy
@DennisKanji254
@DennisKanji254 4 года назад
Spot on!!!
@taefasiri6999
@taefasiri6999 4 года назад
please we need to translation to Arbec 🙂💔
@ArsalanKhan-ki3rx
@ArsalanKhan-ki3rx 4 года назад
Where is didi sabrina cruz
@websurfer5772
@websurfer5772 Год назад
Has any scientist ever even seen a proton or neutron? If not, how do they even know what element they're working with? Also, how do they know they're not just detecting and measuring the electrons they bombarded the substance with instead of the "ions" from the sample?
@emilybentley7502
@emilybentley7502 Год назад
I'm not sure how to answer your first question, but as for the second one: electrons are negatively charged, whereas the ions produced in a mass spec are positively charged. They will move in different directions in response to the magnet - only the positive ions will end up on the detector. There might be other reasons too but I know that one!
@mist2666
@mist2666 4 года назад
on tiktok his name is hankgreen1
@Inadharion
@Inadharion 4 года назад
I spent the first minute coming to terms with your name. The rest of the video was obviously way above my head.
@joshr.5199
@joshr.5199 4 года назад
Where was this in September !? Jkjkjk i did fine
@gibranhenriquedesouza2843
@gibranhenriquedesouza2843 4 года назад
If I had known it would be so hard to find a job in physics, I would have done chemistry ...
@RamdomView
@RamdomView 4 года назад
Annoyed that I forgot the meaning of the wave numbers.
@grad3us
@grad3us 4 года назад
Please, I want CrashCourse Neurology! It would be really cool to understand the brain, just as it was cool to understand computer science.
@uneditedvideos6492
@uneditedvideos6492 4 года назад
what is the "white powder"?
@memlafeder
@memlafeder 4 года назад
Why nobody tasted the white powder? It is easier and instant result. (go hollywood science!)
@lokilaufeyson935
@lokilaufeyson935 4 года назад
I tried to be 1st and I failed. It think I’m about 3rd though 😊
@danteller8282
@danteller8282 4 года назад
I'm not entirely sure if I agree with the way you pronounce "cation", but that's a pretty common area of contention. If we lived anywhere near each other, I would totally use that as a premise to ask you out.
@kevinconrad6156
@kevinconrad6156 4 года назад
Something makes me think that Dr. Chakravarti likes purple.
@Vertigotrueshot
@Vertigotrueshot 4 года назад
what about an Anti-Mass Spectrometer.
@Karajorma
@Karajorma 4 года назад
Well first you need to convert your lab to run on dark energy.
@sama7635
@sama7635 4 года назад
im dumb
@ChadWilson
@ChadWilson 4 года назад
Purple!
@uneditedvideos6492
@uneditedvideos6492 4 года назад
it is that thing people like to comsume?
@zohaibhassan3786
@zohaibhassan3786 4 года назад
Please make videos on history of Pakistan and also on criminology
@joaovitormatos8147
@joaovitormatos8147 4 года назад
Let's put a burning building on the thumbnail. What can go wrong?
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