I also did it as ratio: .3 / 0.41 = x / 0. 47 .... .3(.47) / .41 = .34 as well. If we are within are solution standards and we have a straight line we should be good this way as well
Thank you for the post. How can the Beer-Lanbert's law be used when determining the specific activity of an enzyme (a measure of the purity of an enzyme)?
Are you looking to quantitiate the amount of the enzyme or to just see how fast it is acting? If you are looking for how fast, you could follow either the increase or decrease in the absorbance of the solution over time. This assumes that there is a species in solution (reactant or product) with the enzyme that absorbs some unique wavelength of light. Otherwise, if you're just looking for how much enzyme is in solution you could either create a standard curve or look up the molar absorptivity of the enzyme.
Help with a question ????? A compound absorb light in 227 nm. The standard curve calibration for the compound is Abs = 372x + 0,6432 which the concentration is g/100mL. The technique was analytical standard adding from fluoxetine hydrochloride (C17H18F3NO.HCl - molar mass = 345,79 g/mol). So determine the concentration in mol/L from the analysed solution. Show all calcs progression.
