Was noticing that as well and wondering whether the base of the robot would stable enough to not move (significantly) when properly attached to a stable and rigid surface.
Always room for improvement tho, maybe our (grand)children will one day be able to skip some of the hassle of endless pipetting in standard protocols 😉
@@AliveInTheLab Robotic bio is the future. I hate 96 well plates and it takes a fraction of the time to write a quick program and run it with this than it does to do a whole plate by hand. And if you ever have to run the same thing again, just grab the file and you're good to go. This and robotic remote labs are the future
@@thethoughtemporium and that's why I studied in robotics . Robotics automatisation is inevitable after a certain scale, and even if we got to the point that you can make anything in a GUI , you still need someone to program the robot for adding new components and protocols.
I can hardly believe it... That is the Vanguard microscope i sold a year ago with micro-manipulators. At the time, i was interested in making cloning equipment. I hope the microscope can be fully restored and made extra nice. the glass needs alignment and cleaning.
@Internet Guidance actually i have indeed been thinking about that myself. unfortunately it is not something i can afford right now. time and money are an issue.
My local hackerspace is ruined by the elitist mentalities of the people that have been there longer and became "master instructors" somehow, even though they struggle to hold a .01 tolerance. My 10+ years of aircraft lathe manufacturing experience meant nothing to them. 🤷♂️
Counter point: Or the mechanical vibrations introduced by the motors might be introducing fluid mechanics to the samples that could lead to a discovery?
Wow--you know you're on the really cool side of RU-vid when you're getting Opentrons product placement. I interviewed there a few years back and have been excited for them ever since. Glad to see they're killing it and sponsoring some of my favorite folks out here!
If you like science and art mingling then looking into early electronic music is probably the coolest thing imo. Tons of really smart people in electrical, mechanical, and electro acoustic engineering putting all of the cutting edge knowledge of the time into making music. People like stockhausen using WW2 electrical test equipment to make music. Or people like Delia Derbyshire using magnetic tape in new and experimental ways in a time when there was maybe 40 tape machines in the UK total. Or the synth makers like bob Moog, don buchla, Serg, etc. All brilliant people making the building blocks that all modern synths are built on. Hell, all of modular synthesis on its own is super cool, even today. Like there is a module for modular synths call the squid axon and it's a simulation of how real squid axons work, but applied to a musical context. Its all really cool stuff, well worth looking into! (Don buchla is probably the best place to start for that mix of art and science, plenty of documentaries about him too)
Putting bacteria into agar creates a different kinds of problems, mainly that it cant exchange gasses. If it is aerobic it will most likely just not grow and anaerobic will have limited growth.
Unfortunately no, the way tattooing works involves a pretty substantial amount of ink spread across the surface, and a small amount is carried down by the needle on each stroke of the machine. So there is plenty of 'splash' and spread, and they aren't hypodermic or anything; when tattooing, you simply wipe the excess ink off the surface at regular intervals. Not a good option here 😉
I am going to choose it too. I still need few years to start studying the specific bio engineer. Let me know if at school they teach things he do or not.
Funny enough, NileRed is also moving to a new lab. I actually assumed at the start of the video that they were moving into the same lab, since they have overlapping needs and have collaborated before.
Labs in transition between companies often scrap the old equipment. The computers are generally removed or the instruments are custom, so reverse engineering needed if the manufacturer will not help. I've visited a few of these labs to help the new owners repurpose or salvage equipment. The quality (and original purchase price) of the equipment is generally high. Not sure how how to locate them in general, but lab robots, especially plate and liquid handlers, would be typical finds. Someone will probably know someone...
This is very cool to see! We use an Eppendorf liquid handling robot in our lab and often the GUI is extremely frustrating and limiting, as a handy tip for agar plates, older ones loose a little moisture and things stick better so you might not need a blowout step. You could also let your plates dry out a little when you get a decent hood and that will help also.
YES PLEASE! it should be doable when looking at the link pixelart, and then send give them away as promotion to labratories, no bio enthausiast can resist the urge to scan a biological QR code righy?
Nice work! Just two things that could be interesting for you. Have you thought of 3d printing a petri-dish sized plate with tiny spikes to create small wells on your agar while it solidifies? this could solve the droplet leaking problem. Also, people tend to use alcool at 70% to kill bacteria instead of 100%. The problem with 100% is that it can make some bacteria sporulate as a defence mechanism. This tends to happen less with 70%.
Because it has completely sterilize any air going inside of that robot, making something that can 100% sterilize air is shall we say a tall order. I'm willing to bet money if they made it so it only filtered the air and not sterilized it, the thing would be many times cheaper
@@the_undead yep, the hepa filter is probably very good, plus, for applications like growing cell culture, the slightest bit of contamination can ruin the culture.
I wonder if it is possible to modify human DNA to feature more robust error correction so that it is less susceptible to radiation, and to give individual proteins more robust error correction so that they are less susceptible to prions.
Can you print this and have action that move through Cetus t creating a unique and original changing electrical connection from key points around it changing Mirco electrical flows. If yes contact the poker machine companies because I can pick the basic chip set and they know it.
"to be fair this is not something the robot was ever intended for" I'm going to correct you there, this is something that the designers of this robot probably never their creation would be used to do
If you have those transducers from the sonoluminescence video still might try using those to agitate the agar. If it's anything like cement should work well to level it out before you print.
That lapspace looks great. But it kinda takes a way the "you can do at home in your hobbyist space". Or all the homebuilt tools. The professional options do have merit. Excited to what's coming next tho. Having the liquid be level is a big task in lithography when you spread the photo resist. But it's a much smaller scale than bacteria medium. Perhaps a similar approach works (spinning it really fast)
From the moment I understood the weakness of my flesh, it disgusted me. I craved the strength and certainty of steel. I aspired to the purity of the Blessed Machine. Your kind cling to your flesh, as though it will not decay and fail you. One day the crude biomass you call the temple will wither, and you will beg my kind to save you. But I am already saved, for the Machine is immortal...
Hah, I was just wondering about the feasibility of tip washing after you'd talked about how many you go through. Cheers for answering that curiosity not long after. That's an awesome robot and the new space looks amazing. I greatly look forward to all the cool stuff you're gonna make there.
Our Beckman i7 protocols were used for covid sample prep - all in one sample prep using multichannel manifolds and a span 8 to allow both whole plate pipetting and single well. I use the i7 for mass spectrometry sample preparation, cellular extraction etc. the liquid handlers are a gamechanger. For entire protocols though, the cellario setups from HighRes are where its at.
It would be pretty cool to run a cellular automaton like GoL on actual cell cultures, if such a thing is even possible. Robot could set up initial conditions really precisely.
You should bioprint a string of neuron mold with the florescent activation mechanism(i saw it a while back, you'll have to look it up), you can get them to fire and it will flow around the chain and it will repeat on itself, it's crazy cool. I would bet you could create some really weird stuff with that, who knows maybe it'll come alive and scurry off the plate! haha, kidding, kindof..
You should consider turning down the acceleration to prevent it shaking your table so much. You can change this in /var/data/robot_settings.json. Brought mine down by an order of magnitude and it helped a bunch. I have no idea why Opentrons sets it so high by default.
Is this whole video just a 17min infomercial for this robot and still running RU-vid ads, ad inception. Im just giving hard time you didn't name the robot Bender Bending Rodríguez or just Bender. Then if you get more robots you can get the whole cast of robots (Flexo, Calculon, Hedonismbot, Dr. Perceptron, Robot 1-X, Donbot, Roberto Crushinator, The Masked Unit, Tinny Tim...)
While microbiology is something I have zero interest in pursuing, these videos are super interesting. I'm a botany guy, I don't like dealing with living things that aren't plants. On a related note, I wish the medical field could be made less wasteful in the future. I understand that single use plastics are the best way to ensure something is sterile, but there is an enormous amount of medical waste created every year. Recycling of that plastic waste would be really great, considering it's one of the areas where phasing out single use plastic isn't exactly feasible.
BRO!! You NEED to fix the shaking of the table the Robot is on!! Either get a couple of metal straps and bolt it to the wall, or fix an uneven leg if that's it, but no matter what it is causing everything to shake EVERY TIME THE ROBOT MOVES, you NEED TO FIX IT!!! :) If you don't know what I mean by metal straps they are strips of thin metal (maybe 1/16th of an inch - sorry I'm American, my brain is broken and I only do Imperial :( lol ) that are fairly pliable and they have a line of hole going down the center length of them. You bolt it to the wall and bend it into an L shape and bolt the other end to the table.... this can be VERY GOOD at holding it solid if you put them on all 4 corners and DO IT RIGHT... but it can be hard to do it right... Maybe putting a bunch of heavy weight on the bottom shelf will work... I'm not sure the best way, but everything looks SUPER WONKY for a biology lab if it shakes ever ytime you touch the table, or the camera, or the robot moves... and I feel like it could be causing some of the 'pixels' to bleed away from where you want them :) I hope this wasn't too jerk-ish of me, I just wanna help :)
So, like, they didnt even add into the firmware stepper acceleration to smooth out the movements? I mean, look at that thing shake around! Geeze.... Also, can these companies stop pretending they're doing something special? Like, its just a bog standard CNC style XY plotter with a Z on the head, plus the equivalent of a servo on a pipettor. Yes, its neat, but......IDK for what Im sure you paid for it, I find it lacking....very thanos 'too much thought into what it looks like instead of how it works' kind of vibe. Some CAD wizardry and any 3D printer can print a head to do essentially the same thing..... One thing I still am waiting for with a lot of these rehashes (and I will disparage as long as they want to pretend each iteration isnt a 3d printer with a different head (so basically a 3 axis CNC)) is computer vision. Like we barely have 'oh no stringy mess' detection on SLA printers.....Yet these companies have no problem charging out the nose for the same old firmware with a different UI..... Much disillusion, very disappoint. And they already have (VERY EXPENSIVE) machines that do exactly what that other biolab does with however many of those printers, but in one machine that doesnt need its racks handled between stations. And why not have a robot to do that step anyways? 6dof arms arnt exactly exotic anymore, they can afford all those printers but not a simple pick and place arm? Kind of throws their attention to detail into question honestly....
Great news on the new lab and equipment! Really looking forward to seeing all your new projects there! I love learning from your videos and how you make your projects. How fine can you try printing with that pipette machine? Can you possibly print organics into electronic components with it?
You said the machines are used in REAL LABS. Brother... you have a REAL lab. 😂 Don’t try to short yourself by thinking your lab is not good enough to be considered a real lab. You have a beautiful setup and I would legitimately work for minimum wage just to be able to work with you there. Even if I was just a cameraman or a bodyguard... I’d be all smiles every single day. 😊
Neato! I got pretty far behind on my MPCNC conversion, maybe I'll just buy one of their pipettors *checks price* even just the pipettor is $900, nvm I'll keep 3d printing garbage :p
WOW! Congradulations on your new lab man! Definately has a less jury-rigged bio-lab vibe (To be honest I loved that vibe) But stolked you've got a new hangout.
Regarding half an hour of mesmerizing video (cf. ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-NDKLM-The7Y.html): don't forget, this is RU-vid; people come here to watch ten hour train driver videos. Just yesterday, I listened to half of a ten hour video of crickets and other night woodsy noises. So don't worry, I think you'll have some takers. :-)
