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Simple Bacteria Genetic Engineering

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Genetic modification sounds like something that would be very complicated and difficult to do but the reality is that couldn't be further from the truth. With the right tools and techniques it's something that can be done on your kitchen table if you really wanted. In this video we explore how bacteria can easily be modified to produce a range of fluorescent proteins and even bioluminesce.
More resources:
Building an incubator/heatblock - • From Beer Cooler to a ...
DNA Extraction - • Extracting Spider/Bact...
Running a Gel - • Gel Electrophoresis: H...
Building a Gel Doc - • Building a Gel Doc - T...
DNA transcription and translation - • DNA, Hot Pockets, & Th...
Spider silk project overview - • Spider Beer - Making Y...
Sebastians pages:
Instagram - / atinygreencell
Bionomica labs - binomicalabs.org/
David:
Channel - / @davidishee
Stores to buy reagents:
bio-world.com/
www.phytotechlab.com/
www.goldbio.com/
www.carolina.com/
amino.bio/
www.addgene.org/
neb.com/
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Nebula: go.nebula.tv/thethoughtemporium
Ko-Fi: ko-fi.com/thoughtemporium
Become a member: / @thethoughtemporium
Store: thethoughtemporium.ca/
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Our Social Media Pages:
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Twitter: / emporiumthought
Website: thethoughtemporium.com/
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Опубликовано:

23 янв 2020

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Комментарии : 978

@CeramicSerpent 4 года назад

2020: How to make glowing bacteria 2021: How to genetically engineer bacteria to produce jet fuel

@thethoughtemporium 4 года назад

It's been done. not overly difficult, but I find it boring so won't.

@EctoMorpheus 4 года назад

@@thethoughtemporium I recently read about a kind of "artificial leaf" technology based on cuprous oxide (as a catalyst, it isn't used up in the process) that will take sunlight, carbon dioxide and probably water and turn it into (some precursor of) methanol. Idk if this is your kind if thing, but it'd be amazing to see a video on this!

@GreenCaulerpa 4 года назад

EctoMorpheus Yeah... graphitic Carbon nitride can do this also and is way more environmentally friendly

@SahasaV 4 года назад

March 2021: How to genetically engineer bacteria to produce steel beams. Sepember 2021:

@mrmeadors4661 4 года назад

@@SahasaV When it turns into something else and steel starts growing in random acceptable areas. I wonder if it would be possible for a natural organism to produce things that look unnatural. Like a plant that just thrives in gasoline or oil and breathes in the vapors and in turn produces something that makes itself and the source fire resistant.

@storyspren 4 года назад

"It's cyberpunk as hell" is always at least among the top 3 reasons to do anything in biohacking.

@siiioxide7807 3 года назад

until now... because cyberpunk failed to come close to the hype.

@storyspren 3 года назад

@@siiioxide7807 I was thinking about the genre rather than the game when I wrote that lol

@lucidlywaking7286 2 года назад

biopunk

@DUIofPhysics 2 года назад

Also among the top three reasons not to do it.

@Mintfriction 4 года назад

"You add resistance to antibiotics and a lot of researchers do this" If I die by a fluorescence antibiotic-resistant bacteria, at least I know which people to haunt

@ponkievorster5039 3 года назад

Dont reveal my future plans

@quappo 2 года назад

Fluorescent*

@JGHFunRun 2 года назад

at least it's only one type of antibiotic and not all of them lol

@hadrian2801 2 года назад

@@quappo you're just as bad as the glowing bacteria.

@fakegameryt1234 2 года назад

@@JGHFunRun thanks now I can just add that in my list

@thedankgoat7972 4 года назад

So could you use these proteins and incorporate them into spider silk to eventually make genetically engineered silly string

@SToNeOwNz 4 года назад

Yes, like home-brewing alcohol then having great fun. So a lot like home-brewing.

@MandrakeFernflower 4 года назад

Beer was mankind's first foray into biotechnology 😁

@theapexsurvivor9538 4 года назад

@@MandrakeFernflower technically wines and ciders were, but close enough.

@richard-6920 3 года назад

Also glowing spider webs

@nou1438 3 года назад

@@theapexsurvivor9538 I thought it was mead?

@craigspaulding9711 2 года назад

It’s insane that just a few decades ago this was unthinkably cutting edge technology, but now it’s something college freshmen can do in the first semester of bio lab.

@ImNotQualifiedToSayThisBut Год назад

You don't even need to be in college for it

@ivangarcia-lopez2236 Год назад

yup, I did it as a junior in a public highschool

@devonharvey8414 8 месяцев назад

@@ivangarcia-lopez2236wait really?

@ivangarcia-lopez2236 8 месяцев назад

@@devonharvey8414 bay area, so biotech has strong influence in what we do in class. I haven't heard of many other highschoolers doing it, but just know that some highschoolers are already doing it!

@typhooni3149 6 месяцев назад

And soon the gatekeeping will entirely stop for bio-engineering and we can all do it at home (as this whole project is awesomely about).

@wesstone7571 4 года назад

Someone is going to crispr themselves so they glow, I just know it.

@coagulatedsalts4711 3 года назад

@Heather Petersen i saw someone try on youtube, they failed. they didn't use a virus to deliver it and instead tried to tattoo it onto themselves. it just got digested by white blood cells.

@gamestuff5944 3 года назад

You would have to do that to sex cells because you can't modify 1,000,000,000 cells at once.

@wesstone7571 3 года назад

@@gamestuff5944 you don't have to. I thought if you modified your dna, your body would absorb the changed cells. But if you change them and stick them into your bone marrow, apparently they change you..may not have to hit bone marrow depending on what you're trying to do

@spec_wasted 3 года назад

Failed man, it was bad, I was didn't know the liquid that flows in algae was poisonous

@Uromastyxfanatics 3 года назад

@@spec_wasted 😂😂😂

@chevanc 4 года назад

I wanted to pass on a lab tip that someone brought to our lab a few years ago that blew all our minds, and might help you out. Spreading bacteria is super easy with glass beads. I think they're the same kind that jewellers use for grinding media, they're clear, maybe 2-3 mm in diameter. Basically, you pipette your bacteria onto the selective plate, pour a small bit of beads on the plate, put the lid on, then shake the plate side to side. The beads spread the bacteria super uniformly, and you can spread many plates in parallel. Then, you can dump them out into ethanol for cleaning and re-use.

@speaklifegardenhomesteadpe8783 2 года назад

Awesome!

@trevorbell5070 8 месяцев назад

Oh yeah plating with beads is how I grow up transductions or transformations every time. It’s perfect for getting an even spread of colonies!

@TheRealMoolander 4 года назад

Toss a coin to your teacher...

@obamamanmanman8734 3 года назад

how does this not have a single reply

@julian-io5wl 3 года назад

single reply

@obamamanmanman8734 3 года назад

@@julian-io5wl shutup

@horrificminecraftgameplay5884 3 года назад

@@obamamanmanman8734 shutup

@obamamanmanman8734 3 года назад

@@horrificminecraftgameplay5884 shutup

@5Genjoyer 4 года назад

Hey man, thank you! I want to let you know that because of your videos, I've decided to get my undergrad degree in biology. I've been putting off using my GI bill, and I've been working trade jobs since I got out of the military, but your videos helped me see how cool and interesting Biology and Microbiology can be. I'm starting in the summer semester, so I'm currently brushing up and studying this semester.

@eugeniobonello418 4 года назад

@@miltonferreira9287 its hard to change things up, but honestly give it everything you got. You might feel like and imposter sometimes, but work as hard as you can and you will get through to the other side. Made the decisions to go back to school for computer science and Im never going back :()

@modyosman5059 4 года назад

Sounds great, best of luck.

@markocska94 4 года назад

Good luck!

@saraha8276 4 года назад

Doing my masters in MolBio. Do it, it's so incredibly fascinating :)

@spec_wasted 3 года назад

RIP bro, it is never never never fun, the books and the way they teach it, not fun Talking about India, that's where I'm from

@davidson341 4 года назад

Stop me if you've heard this one before: DIY genetic engineering and buying plasmids, set in a highly capitalistic society. Would you kindly make more of this great content?

@Orandu 4 года назад

A man chooses, a slave obeys...

@SameLif3 4 года назад

Atlas Hugged ur talking about politics

@lefleurdulmal 4 года назад

@@SameLif3 They're both referencing Bioshock, a video game.

@first-last557 3 года назад

"the concept was sound"

@truerain1752 2 года назад

We must overthrow capitalism.

@annonimooseq1246 3 года назад

“What are you doing?” “Steaming my face” “To open your pores?” “Yep” “To get impurities out?” “To let the dna IN”

@YMandarin 4 года назад

well, programming hello world is easier than growing fluorescent bacteria

@ryno4ever433 4 года назад

Not if you're programming Hello World on a PlayStation 3.

@thyTwilightGoth2 4 года назад

@@ryno4ever433 Well, you're not wrong.

@YMandarin 4 года назад

@@kozmaz87 yeah, that's true.

@ahmedalfatih7723 2 года назад

@Heather Petersen try making it by logic gates

@Abcdefghijklmnopqrstuvwxyz1024 2 года назад

It wouldn't be if you had fluorescent bacteria

@ThePiGuy24 4 года назад

cant wait for a C to Gene compiler :p

@masondaub9201 4 года назад

Nah program entire cells in verilog

@starletscarlet 4 года назад

Minecraft for plasmid when?

@ThePiGuy24 4 года назад

@@starletscarlet I don't think bacteria has enough memory for the JVM :p

@theapexsurvivor9538 4 года назад

@@starletscarlet how much rna are you willing to allocate for Java?

@mme725 3 года назад

1 year late, but "GeneCC"

@tumbleddry2887 4 года назад

"EVERYONE'S first experience with genetic engineering"?!....I really am out of the loop

@romajimamulo 4 года назад

Everyone, who has experienced genetic engineering

@DanaTheLateBloomingFruitLoop 4 года назад

Haven't you heard? It's all the rage with the youngsters.

@reggiestickleback7794 4 года назад

Tumbled Dry AP biology in American high schools

@theshuman100 4 года назад

get with the times nerd. all the cool kids are doing it

@sreeser3512 4 года назад

He means in classes. AP and collegiate biology classes regularly perform this experiment. I've done it twice as a student. Bacteria are cool. You can convince them to make virtually any protein, provided you can obtain or produce the gene to do it.

@cauhxmilloy7670 4 года назад

Semi-related: any update on your lactose intolerance cure?

@handsanitizermk.268 4 года назад

Adding myself to qestion

@CarlosBunn 4 года назад

I wanted to know more about that myself. I'm not lactose intolerant but my girlfriend is. Something like that could give a better quality of life to millions of people. That should not be confined to a youtube video.

@imperialphoenix1229 4 года назад

I agree, I'd like him to talk more about it

@randalmarshik4320 4 года назад

👀

@lancer2204 4 года назад

Yes, I'd love to hear some news too.

@pravda9646 3 года назад

We did this in our biotech (high school) class!!! I was really surprised to see you doing this, I thought it was a basic experiment. Like a baking soda volcano.

@thethoughtemporium 3 года назад

It is. But I needed to show it once so I can reference back to it in future episodes.

@marsoz_ 4 года назад

I like to pretend I understand the things being talked about in the video

@WandererHermit 4 года назад

MarsOz who does not

@username-rs4vf 4 года назад

Eventually you will

@shafthespaceegg 4 года назад

The His6 tag after the open reading frame on the plasmid encoding the red fluorescent protein is six codons encoding histidine. Histidine will bind to metal ions like Ni^2+ allowing you to separate the recombinant protein by running lysed cells on a nickel column. The recombinant protein will stick to the column and everything else will run through. The recombinant protein can then be eluted by addition of imidazole (histidine has an imidazole group in its side chain) which will compete with the histidine residues for binding to the nickel column and the recombinant protein will be pushed off of the column.

@thiagokawano1618 3 года назад

Everyone here does.

@spacekid9680 2 года назад

Me too a lot of it just sounds like interesting gibberish

@alexb554 4 года назад

Damn we're doing this experiment in biochem lab this quarter. Super cool to see this pop up in my feed

@planktonfun1 2 года назад

make glowing human babies

@Ryan6.022 4 года назад

You and applied science are the only channels I have notifications on for. Edit: 9:39 is a little too real for me.

@AsmageddonPrince 4 года назад

What other channels do you subscribe to?

@AtlasReburdened 4 года назад

Dude, youre missing Tech Ingredients. You can't leave them out of exquisite original content club.

@uberawsome3696 4 года назад

I love abm.

@MrTikiTheKing 4 года назад

I find it interesting that by watching these videos for the past year I have kind of learned what he is talking about. Without looking anything up or really doing any external research I am learning. I believe that people learn to speak using this same method.

@filippovolpe746 4 года назад

Ok it's basically assembly.

@benjaminmiller3620 4 года назад

Pretty much. If all the instruction paths are being executed concurrently.

@GRBtutorials 4 года назад

@@benjaminmiller3620 Then, it's like HDL for FPGAs and ASICs?

@woofcaptain8212 4 года назад

Lol as a computer science student biological processes are so fascinating. There are so many parallels to the way we engineer computers.

@DaveSmith-cp5kj 3 года назад

@@woofcaptain8212 Bioinformatics is also critical in helping us understand what the heck is going on and what to do next.

@TheWatcherinthewalls 2 года назад

Soon my guys, soon we will get cat girls.

@thomasrogers8239 4 месяца назад

"daddy why do we look this way?" "Well honey that's because our forefathers were idiots and didn't make a backup." "Terrifying!" "Indeed."

@Lasersplitter 4 года назад

I've been following videos like this for years because I find this whole process and the science behind it fascinating. Sadly, the country I live in has such strict laws regarding genetic modification that it's practically impossible for any private person to do this.

@RomanLeBg 4 года назад

I feel like its an excuse to not try lol I'm sure you can do some small experiment without problem

@Lasersplitter 4 года назад

@@RomanLeBg If the equipment weren't so specific, sure. But "I swear, officer, I just ordered these plasmids and the CRISPR starter kid from overseas so I coud display them on my shelf, I had absolutely no intention of using them" doesn't sound like a very good excuse

@MANUakaSHUFFY 4 года назад

@@RomanLeBg Nah. you can get into really big legal trouble here (Germany). Even ordering stuff like Glowfish is prohibited and punished pretty hard. I´ve studied biotechnology and must say, that i´ve relaized that there really is no other legal way to do it, than to do it in a approved lab etc.

@reelbytes6447 3 года назад

Maybe I should stock up on crispr in my country

@nightslasher9384 2 года назад

@@MANUakaSHUFFY Go to another country and bring it to Germany. No borders remember.

@nadey_nate3441 2 года назад

I remember doing this in my biotechnology class, back in high school. Fun Times!

@emilyruth4796 2 года назад

I did this in high school and it was easily my favorite lab

@909sickle 4 года назад

"Less is more with DNA" That's what my grandpa used to say

@spacekid9680 2 года назад

Haha inbreeding

@lifeisgood339 4 года назад

Your videos are so freaking great! So descriptive and informative always, keep up the hard work!

@BazilRat 4 года назад

I am looking forward to Styropyro's video, whether it succeeds or not!

@crackedemerald4930 4 года назад

I think it's been out for a while.

@BazilRat 4 года назад

@@crackedemerald4930 Odd, I can't find it!

@William_Hada 4 года назад

This video is freaking awesome! Extremely interesting and well presented, I learned so much. Thanks for sharing.

@UrbanGT 2 года назад

Love that this information is free on the internet, thank you!!!

@lucywerner 4 года назад

This video is so informative! This semester we were actually supposed to extract and do various folding experiments with eGFP from e. Coli, but due to COVID our uni is closed for the time. Our professors were kind enough to take videos of the whole process, but it can't really compare to the real thing. Thank you very much for making this video!

@israelcarrera7287 2 года назад

I did this when i was 14-15 i was the only one to get it right in my advanced bio class.

@lourainevillalon3852 2 месяца назад

hello, sorry for being really lateeee, how did you obtain the bacteria? did you experiment with e.coli too? can i use the salmonella bacteria in raw chickens in this experiment? been wanting to test this out for myself. thanks!

@muzehack 4 года назад

Can someone make some fluorescent yogurt bacteria? That would be cool.

@jaxblonk5127 3 года назад

Yogurt generally isn't a monoculture but I love the idea of that.

@ponkievorster5039 3 года назад

Its already been done

@patrickjenkins9874 4 года назад

I appreciate the use of those 3D printed tube holders :). P.s. love your videos. No other RU-vid channel like it that posts such a wide variety of amazing science experiments with such a high level of cool sci-fi feel to them :)

@LifeMaxxingOfficial 10 месяцев назад

Do you know where I can get the file for it ?

@JCdied4Uall 4 года назад

I feel like I learned so much watching this! Thanks!

@aidentheis8303 4 года назад

Litterly just did this in my bio class, great video!

@leftysheppey 3 года назад

Today I learnt people just ship eachother DNA like its nothing. Just wild

@unclekanethetiberiummain1994 3 года назад

We've been shipping DNA to eachother since the dawn of time. ;)

@WackyrDrago Год назад

@@unclekanethetiberiummain1994not you though 😢

@eingyi2500 10 месяцев назад

I shipped DNA to your mom the other day

@glitchy_weasel Год назад

Very awesome. Didn't though genetic modification was this straight forward.

@Queer_Nerd_For_Human_Justice 3 года назад

Dude. Rad as hell. I agree the flower concept is amazing and the colors he got so far are incredible. I'm no chemist or biologist, but I am an artist, and I am in love with the vibrancy yall are able to produce.

@Forensic1Man 4 года назад

Hi! This has been a really, really great demonstration and explanation of gene modification and insertion. It is so good I will probably use many of your descriptions in my classes! Good job!

@mozkitolife5437 4 года назад

8:25 I got visions of high PhD students in the StonyLab. "Dude, these agar plates are giving me the munchies".

@scooterglass4497 2 года назад

Thank you. I have been fascinated with bioluminescence and fluorescing proteins.

@ValeryDjondo 2 года назад

Thank you for the web sites assessment sharing. You are realy generous to share your experience and advices...

@LeDracodon 4 года назад

I like the programming analogy when tinkering DNA is involved, it just when people begin to say that DNA is a manufactured code which is not good.

@gh0stmast3r 4 года назад

Also with programming it was built by humans and is about 200 years old and biology is literally a language built by the universe and stress tested for millions of years.

@SolarShado 4 года назад

No analogy is perfect. That seems the be the bit that trips people up. And while this one seems to be pretty good, it actually starts to break down really quick once you start digging into the details. The "runtime" for DNA is way more complex and unpredictable than any digital computer.

@fluffy_tail4365 4 года назад

@@gh0stmast3r Which considering there is no design in it makes it actually quite shitty and fragile. It's stress tested for numbers game, not individual reliability. Just look at promoters not having any particular common pattern.

@SameLif3 4 года назад

fluffy_tail programming can be though, even though they said it’s not(revolutionary)

@llewsub 2 года назад

I know nothing about microbiology but I want to learn enough that I can do a project like this at home. Should I take a entry level bio lab at school or can I learn all of this on the internet?

@skoci5159 2 года назад

Yes take it, and on the internet you can do everything you want to learn

@dejayrezme8617 4 года назад

This is a really amazing video. Thanks so much!

@peregrinussolutionsllc6010 4 года назад

As usual with your channel, Great video! Thank you for sharing your knowledge on this very interesting subject. I previously worked with quantum dots and the spectrum of vibrantly fluorescing test tubes brings back pleasant memories :-)

@viqtor23 4 года назад

The Thought Emporium: Let's make antibiotic resistant bacteria! Everyone else: WHY? The Thought Emporium: They will glow cool.

@Flying0Dismount 4 года назад

Isn't mutating DNA in labs how they start the zombie apocalypse in all the movies?

@gh0stmast3r 4 года назад

While it's technically antibiotic resistant bacteria this ain't the stuff you get from the pharmacist.

@Jesus-eu4gn 4 года назад

@@miltonferreira9287 makes it less scary. Imagine multi colored glowing zombies running after someone. "Pride" zombies.

@Jesus-eu4gn 4 года назад

@@miltonferreira9287 scary but easier to detect at night? I hope its one of those weak slow moving zombies, not those world war Z types.

@ummmhelp 4 года назад

@@Flying0Dismount zombies are stupidly impractical they would be wiped out in 2 to three months in real life

@happycryingcat3101 4 года назад

Bacteria: Exist Humans: Art

@jofa4050 4 года назад

where was this video when i was in 3rd semester? thank you and great content as always!

@cookie_pixel 4 года назад

aaah!! so excited to do this at university! this career is truly wonderful!! subbing ♥

@themadlad5577 4 года назад

Loved this but as a microbiologist I do need to point out a few errors in your antiseptic methods. You shouldnt open eppendorf tubes with the same hand as touching the lips could contaminate the containments. 2; you should have a flame nearby to direct airflow away from plates when you're pouring media. 3; you should use a hokey stick type spreader and a spinning plate for spreading rather than Q tips :). You'd get a much better spread. There are other things like always having correct airflow (flow bench/ work near flame). Also for storing your bacteria you might be better off if you plated your bacteria and inoculated a couple of plates from which you could collect bact via loops later should u need to. I loved the video tho, keep it up :)

@themadlad5577 4 года назад

Oh I almost forgot; leaving the media/broth container cap on the ground can deffo contaminate stuff u should also watch out for that :)

@thethoughtemporium 4 года назад

There was a flame going off camera in many of these shots. Also they weren't q-tips, they're individually packaged sterile swabs. I hate hockey stick spreaders honestly, the swabs work like a charm. The entire lab is a positive pressure room fed by a hepa filter, and there's a second hepa filter next to me. Also there's a massive uvc bulb above the workspace that I run occasionally to nuke the whole room. The contam on that plate was likely because I waited too long and let the media cool too much before adding the charcoal. Had gotten busy doing other stuff waiting for the autoclave to cool. Contam on the rainbow is because of how fricken long it took to make that so the open time was high. I store plated bacteria as well, but I prefer to store bacteria as stabs. That said, I keep stabs, plates and pure DNA of every plasmid I have. I was taught in school to leave lids face up on the ground if I can't hold it with my pinky, and have almost never had issues. I am looking to add a filtered air blower box/laminar flow box onto my work table to help lower contam rates even further for doing more delicate work like plant tissue culture. Thanks for the tips though and I'm glad you liked the video :)

@themadlad5577 4 года назад

@@thethoughtemporium sounds like you know what you're doing! Good look in the future and keep the good content coming ☺️👍👍

@MishiMIshaniz 4 года назад

Man, you're really making me wish I took bio in highschool

@joleif4970 4 года назад

Your Channel is one of those wildly insane ones on RU-vid - your experiments cross so many "normal" boundries and I absolutley adore it. It's a great time to be alive where you can just sit in front of your PC and watch someone play around with bacteria, lmao

@6YJI9 2 месяца назад

Bruh, I'm a network engineer/cybersecurity consultant/among other things, and I've always had an interest in becoming a biohacker just never really had the time & money for it. After watching your video which has definitely calmed my anxiety of feeling overwhelmed from how complicated the process just -has to be- I realized that with my IT background this will actually be an awesome and dare I say easy hobby for me to pickup (referencing knowing how to splice a UDP/TCP packet, and its similarities to gene engineering). Definitely hit that liked button and subscribed, looking forward to watching all of your videos, and will for sure try to send some coffee funds when I could afford it (let's just say I have a list, but you've definitely been added and easily in the top 3 influencers I'd like to support when I eventually could).

@staticinteger 4 года назад

This video was so helpful for noobs like me interested in biology and genetic engineering. Thanks for putting this together! Keep up the great work! :D

@gebbert2245 4 года назад

My teacher is searching for german videos like this😅 I watch your videos since 2018 and really like them but many germans don't understand english and please keep on making these Videos🙃

@engineer0239 4 года назад

Shame on every german who doesn't understand english. It really freaks me out when I want to show my friends such an awesome video and they just don't understand it...

@dan8t669 4 года назад

Dann sag den Piefke sie sollen über den Tellerrand schauen und Englisch lernen.

@ChristophPech 4 года назад

Learning English as a German is easier than learning genetical engineering since the languages are very similar.

@xxblackwhitex 2 года назад

I liked the DIY centrifuge! Cant go as fast as the mini you showed yet DIY science is the best kind

@RMV_99 Год назад

These videos always intrigue me, since I'm trying to get a degree in microbiology.

@silverfoenix 4 года назад

Mom: What are you doing? The Thought Emporium : Painting with life...

@LiamAnjewierden 4 года назад

Already waiting for pyro's video!

@happynightmare2332 3 года назад

I dont know the first thing about genetic engineering or biology but I love watching these. They're so interesting

@trailblazingfive 4 года назад

Great video; right on schedule - as you promised. Can you recommend any literature about designing DNA for in vivo production of compounds of given chemical structure?

@andreasstuermer4946 4 года назад

Usually you find out which organism produces the compound and clone the enzymes it uses. Structural biology isn't quite there yet, for a lot of things

@armedandredee 10 месяцев назад

Can this be done to, let's say, human skin cells? I can hear it now. "Hey honey, check out my lightsaber!!!". #afaf

@alexlandherr 4 года назад

This bring back memories of my graduating high school project which examined the bactericidal effect of capsaicin on a variety of skin bacteria (plenty of control flaws but I couldn’t afford a single strain of bacteria or pure capsaicin). In my study I did *plenty* of agar plates.

@acompletelynormalhuman6392 4 года назад

17:50 that's a really fitting Express in considering the last video you posted

@AngDavies 4 года назад

Why are some segments of the plasmids read backwards and some forward/on one strand Vs the other? Edit:seemingly, going by arrows

@CaptainBeardless 4 года назад

DNA is read in one direction-- from the phosphate end to the hydroxyl end (5' to 3'). The two strands that make up DNA run in opposite directions (anti-parallel). If you've got a double stranded DNA helix going "up and down", the "left" one is able to be read "up" and the other read "down". Since DNA is read in groups of 3, there's 3 ways to read a single strand. as an example, if you have "ATCATCATC" you can read it "ATC...ATC...ATC" or "A...TCA...TCA...TC" or "AT...CAT...CAT...C". You also have a reverse strand "TAGTAGTAG", which can be read 3 different ways. So there are 6 unique ways to read DNA. For further information, look into "reading frames"

@macrochaos 4 года назад

silk beer coming soon me: YEEEEEESSSS

@philippthill6501 4 года назад

I did this too in my biotech undergrad. was super cool. We used a lot of weird fluorescent proteins for our iGEM project, even one with an ubiquitin residue which has a half life time of only 20min so you can see with your own eyes how the production of it stops.

@mystwalker479 4 года назад

YESSSS BEEN WAITING FOR THIS

@MrHocotateFreight 2 года назад

Grow little bacteria at home, pick your color and pit them against each other in a race to cover the most space!

@applepie9806 2 года назад

OOOHHH that is so cool. There's yeast that can make beta carotene??? Can we use them in bread??

@inversegaming6238 3 года назад

Dude u say it’s so simple I am going to have to watch it this repeatedly

@TheFlacker99 4 года назад

Wow! Plasmids, bacteria, etc! Would you kindly make more videos on this?

@sarchlalaith8836 Год назад

I would very much like glow in the dark oak trees... I would pay

@sparkz6381 4 года назад

I remember doing this in biology to make bacteria glow in the dark

@rickfordmorningstar130 4 года назад

back in my day, we cut apart frogs in biology and Margaret Mary fainted XD

@brandonwest4168 Год назад

I remember doing this in my genetics lab! Fun stuff!

@TheArcSet 4 года назад

An interesting video. Thank you for sharing it.

@WandererHermit 4 года назад

Aren’t DNA sequences just LIFE CODE

@merciragasolina1800 4 года назад

And code language is chemistry.

@theperfectbotsteve4916 2 года назад

I know it’s not legal but hypothetically could you make a human glow? Like A really cursed baby build a bear

@MicahPachirisuGuy Год назад

how would you get human cells? dead children?

@eingyi2500 10 месяцев назад

@@MicahPachirisuGuymy taking samples from any living human being

@staticinteger 4 года назад

So excited for this video! :D

@richleyden6839 4 года назад

Thanks for the supplier list. I wish I had that a year ago. I bought most of my supplies on eBay but I would need to find a different seller for almost every reagent.

@ClydeShaffer 4 года назад

Better wash your hands fast, here come the TURBO E.COLI

@dr.q7472 2 года назад

Cool mod Steam link please

@Paganforge Год назад

Big fan of Sebastian and Binomica.😊

@crtika123 3 года назад

This video is soo good at this time, while i'm missing out in my microbial genetics lab because of COVID

@TreyPDB 4 года назад

changing my dna so I'm not related to my dad, thanks

@verumgaudium1564 3 года назад

*OOF*

@synergy021 Год назад

Buying colored highlighter markers for a few bucks seems cheaper and faster.

@userou-ig1ze 4 года назад

awesome to see styropyro collab, amazing

@markmekken8230 4 года назад

Nice video! :) I believe that the non-transformed colonies you mention at 17:58 are perhaps not due to rapid mutations, but rather the secretion of beta-lactamase, the ampicillin resistance protein, from transformed bacteria. The antibiotics around transformed colonies will be degraded, allowing growth of non-transformed bacteria (satellite colonies). It’s a common problem using only ampicillin, although I usually see them slightly smaller after overnight incubation than on your plate. Just to be cautious, pick from the middle of your biggest colonies if you can’t select with UV or another antibiotic like carbenicillin. It will increase your odds for succes :)