Isolating a single spore under the microscope

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This video shows the breakdown of how I isolate a single spore from a wet slide using a pipette. Enjoy and MUSHLOVE
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Опубликовано:

3 фев 2023

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Комментарии : 47

@robbyhanlon Год назад

Gary, I’m pretty sure you are the only dude on RU-vid doing this kind of in depth content. Believe me, I’ve searched. We appreciate it. I am wanting to understand the process of breeding more and there just isnt a lot of content out there.

@FreshfromtheFarmFungi Год назад

thanks for watching and following along! I am building up to a new method of breeding can’t wait to share! 🙏🏻🍄❤️

@diegoramosg Год назад

@@FreshfromtheFarmFungi great videos!!!!

@philkennybr 2 месяца назад

Is the new method out yet?

@InconspicuousOwl Год назад

This is the one I've been waiting for! Cx

@tonystewart7624 Год назад

It's because Gary is playing with fire, isn't it? Lol

@bennynutts16 Год назад

thanks for sharing Gary!

@HChandler2010 Год назад

Now I gotta figure how to justify buying a microscope to my wife 🤣 she'll understand 🫡

@triple_gem_shining Год назад

Shit man if it's business purchase she doesn't really deserve a say 😂😂

@AaronAlso Год назад

Them is some skills, my hands just aren't steady enough.

@philkennybr 2 месяца назад

Super cool technique!

@moisesaramos3229 Год назад

Gary is finally showing his gangster stripes. Digging the metamorphosis, also I knew he wasn’t just some gourmet cultivator.

@tonystewart7624 Год назад

Thank you for another great informative video Gary.

@JimmyGreen-wx6gt 7 месяцев назад

Awesome video

@JUSTINOURSE 11 месяцев назад

Thank you so much for showing how to narrow a Pipette. I didn't know that this was possible till now.

@FreshfromtheFarmFungi 11 месяцев назад

learned it in 6th grade chemistry class I am thankful to have had such a trusting instructor looking back on it! 🙏🏻🍄❤️

@amanitaocreata4401 Год назад

This is why you're becoming my favorite to learn from

@FreshfromtheFarmFungi Год назад

thanks for the kind words! MUSHLOVE

@sporgymushrooms9656 Год назад

Great video Gary! This makes me wish someone would make an iso printer that could print single spores perfectly spaced out on agar plates. Would love to see a video about strengthening genetics and principles of inheritance.

@FreshfromtheFarmFungi Год назад

hah glad someone figured it out 😎 this is my exact project! Kind of… stay tuned once I get the funds I will work on this 👍

@tims-pt1yy 11 месяцев назад

Great video😁

@evil8anana902 4 месяца назад

Bro before the video even started I saw the spore print in the petri dish and I woke up my daughter saying "that's effing genius" lol.

@FreshfromtheFarmFungi 4 месяца назад

thanks! I have a large library and making them in dishes also helps organize 🙏🏻🍄❤️

@evil8anana902 4 месяца назад

@FreshfromtheFarmFungi I'm working on the organizational part myself. Do you happen to know is there is such a thing as a screw on lid for petri dishes? I hate having to tape everything up haha.

@marlinmixon3004 Год назад

I watched your video where you divide a spore solution multiple times until you get a low enough spore concentration to easily isolate single spores. I thought a bit and you might be able to do the same thing with sticky tape. Make a spore print on the sticky side of sticky tape, then line up a bunch of sticky tapes in a row and touch the spore print side to a fresh piece of tape sticky side to sticky side and pull it apart which probably removes half of the spores then grab a fresh piece of sticky tape and touch the last piece of tape together with the new piece. Eventually you get down to single spores. Not sure if a single spore on a piece of sticky tape is useful but was an interesting thought exercise.

@phenofarmseeds5280 Год назад

Awsome

@coloradodirtbike5930 Год назад

Such highly valued info that very few people ever show or even know how. You are a master Gary and your expertise and knowledge really is endless. Thank you for taking the time to teach others.

@ErikPfau 5 дней назад

After isolating a haploid, and growing it out on agar, and combining it with another haploid, will the entire monokaryon become a dikaryon? Or only where they fuse together? Can you make a transfer from the farthest side and it still be monokariotic or does information travel through and know it has mated?

@Milkex 7 месяцев назад

MAP gas is sold in yellow, propane tanks are blue

@benjamindejonge3624 Год назад

Omg when you think Einstein figured it all out , somebody comes and says hold my beer

@syberphish Год назад

Hey Gary, what's a good reference for identification based on size and other spore attributes?

@ScottWConvid19 Год назад

What is the advantage of isolating single spores over allowing spores to randomly mate on a media plate or other nutritious matter? Is there a way to determine which spores will have better genetics?

@FreshfromtheFarmFungi Год назад

the advantage is that you can get more variance - with random selection the stronger genetic expression will prevail and you end up with similar genetics (not necessarily a bad thing)

@triple_gem_shining Год назад

Bro!!!

@kainophile5887 Год назад

I was wondering if anybody went through this kind of trouble to get haploid spores. Do you make a practice of doing this?

@FreshfromtheFarmFungi Год назад

Not really, I am making a better process and filming currently- it has been a couple years in the works I don’t enjoy tedious microscopy but I do enjoy making videos 🙏🏻🍄❤️

@revolioclockbergjr8482 3 месяца назад

Can this be achieved with a hypodermic needle?

@FreshfromtheFarmFungi 3 месяца назад

yes I think with a steady hand

@MonsterMushhead 9 месяцев назад

Is an isolated spore syringe liquid culture?

@FreshfromtheFarmFungi 9 месяцев назад

no liquid culture is different- watch this video it may help ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-x1b1hEX8ABM.htmlsi=5iM4QRnXMocRt_GM

@nubletten Год назад

First time i hear about this is from you. Imagining a cross between cordycep militaris and lions mane that grow very well in wood and eat pests. Also it would look like a cordycep stalk with lions mane cap or reverse :)) This have crazy potential imo.

@FreshfromtheFarmFungi Год назад

hah yes but would be very difficult to cross ascospore with a basidiospore - genetic modification required 🧏‍♂️