+Andrew Areva We re-recorded this video a couple of years ago with higher resolution. Check it (and a lot of other microscopy videos) here: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-1Q5V442V7xI.html
Really nice video, thanks for uploading this. I suggest this to anyone who is going to use a confocal as its going to make your life a lot easier and will make it all a lot clearer. Thanks
awesome vid, thanks to iBiology and Nikon! Wish the resolution was higher though. Crazy to think that after a few years (from the date of this video in 2010) laser scanning confocal has gotten so efficient with the new generation of insanely fast galvano-resonance hybrid scanners that spinning disk is getting phased out since you can get pretty much very close frame rates at comparable resolutions (something like 30 fps, 512x512) without the small compromise in optical sectioning that you get in spinning disk (which is not as bad as some people make it out to be though). Although 2-photon's still the boss, if you've got the $$$$$$ hehe
Thank you for the very helpful video. I have one question.1. Why do we limit to 100µm imaging depth by confocal microscopy? Does imaging depth get better by decreasing pinhole size?
I am stuck, can't get it. trying hard to understand how to see molecular interactions using fluorescence and confocal microscope. is there any possibility to do a video before this and explain basics of how light interacts with specimen?
excellent video..i have a question please,i want to do a fluorescent exerience with confical and 1 EMCCD camera ..what is the difference between using 2 camera or 2 ...thank you
