Microscopy: Two Photon Microscopy (Kurt Thorn)

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Learn more: www.ibiology.org/talks/two-ph...
This talk introduces two-photon microscopy which uses intense pulsed infrared lasers to image deep into biological sample. It can be used for imaging thick tissue specimens or even imaging inside of live animals.

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2 авг 2024

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Комментарии : 59

@hughxiao7078 5 лет назад

10:10 was the moment the advantage of two photon microscopy clicked for me. 16:37 is another great point.

@OsamaOSadolla 4 года назад

mvp

@1239752 4 года назад

Much better than my university's newbie lecturer(surprisingly hired at US top 30 university) Wish he watch this and learn how to teach.

@fernandamonteiro5181 7 лет назад

EXCELLET explanation!! no doubts left behind, very well organized and fulfilled with information! great job!

@alvaroperona6134 2 года назад

Thank you so much for this video!! I was 100% lost on how the multiphoton system worked.Very clear explanation.

@abdullahiibrahimuba1137 10 лет назад

Very complicated but well explained technique. Good job!

@sr2109 5 лет назад

Using the talk for preparing for exam. Really helpful! Thank you

@johnbates2709 7 лет назад

Great presentation, very clear, thank you

@TheWupperdo Год назад

Excellent explanations--didn't realize how much I wanted to use multi-photon microscopy until seeing this video!

@WALLEHua 7 лет назад

excellent lecture! very clear and easy-to-follow

@beflatminor 7 лет назад

Very well explained and helpful. Thank you!

@ClaraL27 3 года назад

SO clear and helpful- thank you!

@TheHerofruitlunch 10 лет назад

Thanks for this, the basic principles are clear for me now!

@sashatolstoy531 9 лет назад

Excellent and easy-to-follow!

@deebadubbie 6 лет назад

Thanks for a great talk!

@colinbarnes705 15 дней назад

Still a great vid 10 years later

@gillenzfluff8380 6 лет назад

Amazing stuff thank you.

@aidated 5 лет назад

Brilliant! Thank you!

@samanthaamat4976 10 лет назад

Very thorough, thank you!

@muthuveljothi4619 6 месяцев назад

Extraordinarily explained 👍🏾

@danielarmbruster94 3 года назад

Awesome, thank you!

@kadamnagesh5155 5 лет назад

Very good information.. Easy to understand.. Thanks

@Kimya_el_tabkh 3 года назад

Great lecture. Thanks

@Rhioah 10 лет назад

Thank you!

@Abyssale90 8 лет назад

THANK YOU!!!

@milophilic5927 2 года назад

Super helpful and logical! thanks !

@gVaiwa 5 лет назад

Easily comprehensible, thanks a lot :)

@frankclausen2506 5 лет назад

wow - It's so nice to se another way for using second harmonic, that just PTK. dubling.

@1point689 4 месяца назад

Great lecture!

@charme2377 6 лет назад

excellent video..i have a question please,i want to do a fluorescent exerience with confical and 1 EMCCD camera ..what is the difference between using 2 camera or 2 ...thank you

@mariaguadalupedelrocioherr9722 9 лет назад

Thanks :D ... Good explanation.

@HayagreevSivakumar 2 года назад

thank you

@oludhe7 6 лет назад

Very good presentation

@crewberggren7669 2 года назад

10/10 In every category

@zainhassep7908 4 года назад

Excellent

@prananditabiswas4599 9 лет назад

Thanks for the excellent explanation. I have a doubt : two photons are two different quanta, even if they arrive simultaneously, would they not excite two different dye molecules with the same energy and not the same molecule with twice the energy ?

@kunlin579 6 лет назад

From my understanding, that's the trick of 2 photon, at the focal plane, the density of the photons is so high that 2 photons will hit the same molecule. Outside of the focal plane, the likelihood of 2 photons hitting the same molecule goes down, therefore photo-bleaching is minimized outside of focal plane

@poodook 4 года назад

There is no real upper level (excited-state) corresponding to a single photon’s energy and therefore there will be no absorption.

@artsmith1347 3 года назад

Agreed: 1) Excellent explanation. 2) I had(?) the same doubt. It doesn't square with my understanding of how excited states happen ... the reaction of a molecule to being hit with half the energy needed to excite it would be: "hold my beer." It appears my understanding is incomplete because they are making it work.

@artsmith1347 3 года назад

So this is ionizing radiation at wavelengths far longer than those for x-rays?

@hellenishikawa7841 9 лет назад

HI it will be possible to have the ppt presentation for didactic propose? It will be nice! Thanks Dr. Hellen Ishikawa-Ankerhold LMU Munchen

@iBiologyTechniques 9 лет назад

Hellen Ishikawa Dear Dr. Ishikawa-Ankerhold, unfortunately we are not able to give out the powerpoint presentations. If there are particular slides you are interested in let us know and we'll see what we can do!

@dr.vannostrand411 9 лет назад

any idea where i can get that graph at 6:25? the link to it doesn't work

@iBiologyTechniques 9 лет назад

Hi Five-Sigma! You are right - the link doesn't seem to work anymore. I contacted the speaker and he suggested these two similar (but not the same) graphs: www.nature.com/icb/journal/v88/n4/fig_tab/icb2009116f1.html research.stowers-institute.org/microscopy/external/Technology/NLO/index.htm Sorry that we cannot find the exact one used in the talk!

@dr.vannostrand411 9 лет назад

Thanks very much! Those graphs are great!

@edoardodimprima7519 2 года назад

@@iBiologyTechniques the links seem broken now. Thanks a lot for the great lecture!

@poodook 4 года назад

22:40-22:45 this is not truly the reason, in my opinion. A more appropriate explanation would be that the two-photon cross-section is extremely low and thus high intensities are a necessity for inducing two-photon absorption. Two photons will always be present, but the absorption will not be stimulated unless there is a high density of photons, owing to the low cross-section. In fact, this cross-section will become orders of magnitude weaker for even higher multi-photon events.

@disisnups 6 лет назад

good explanation, he speaks like sheldon cooper :)

@ashutoshmishra1554 3 года назад

Who dislikes such videos

@cokecamilo 9 лет назад

Are you from the future!?

@skoolscribe3918 3 года назад

So the goal is to measure photon from extraction rather than defining photon by compression however a photon compression engine will suffice most excellently for any security system weather it be for a spacecraft or for a human body.

@skoolscribe3918 3 года назад

Next time just present the source to your data and include user interactivity

@rockgaming1440 8 месяцев назад

This is violating Einstein's statement for photoelectric efect

@souvikces 6 лет назад

Wonderful presentation, except the occasional tongue-clucking "sucking" sound (for instance, see 25:06, 26:19, 26:38, 26:50). This sound can be annoying for many audience. Thank you and I hope you take this suggestion positively.