Leishman Stain Procedure and Blood Smear Preparation for DLC Count 

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Please don't squeeze the finger while drawing capillary blood as the tissue fluid oozes out and mixes with the blood thus diluting the blood sample giving false results.

First i want to thank you a lot for this video, it helped me learn a lot! And secondly I want to ask, Why don't we put a cover slide on the specimen before putting it under the microscope?! Hope you answer me soon!

First drop of blood is discarded right ✅ Thank you sir

Sir we never take first drop of blood ....we have to wipe it

Why you are not adding cedarwood oil

You can mix it by blowing also

No need of methanol fixation for Leshmin stain, its alcoholic stain

Sir.. can I take a short part from this video to make a lecture for kerala higher secondary examination?

Thanks a lot 💓

Thanks sir from Bihar🙏

Very Helpful video 🙋🙋

Thank you so much sir🙏

M. P. Stain reagent A and b hota hai sir red or blue konsa use pehle hota hai btaye

Sir aap finger name galat bol rahe ho ek middle finger hai ek ring finger

Thanks sir... This help me alot🙏

Just found your video... The best video.. Sir can you please explain how to use oil immersion.... Please😫🙏🙏

How to prevent rouleaux formation ??

Thanks.

Thank you so much sir

This method is very egy 😊

Metanol sei fix bhi karni hoti hai slide

Good Job sir 👏 ( Morning me mera practical exam Hai 😅)

Thank you very much sir ji

Nicely explain

We dont have to rinse it completely with distilled water na otherwise the slide will be washed up completely and we will get negative results so its better to put the thumb on the slide so that it shouldn't rinse completely otherwise it will show wrong results

Distilled water kitne drop daalna hai wo bhi bataaea

thank you so much for this video

Sir washing method galat hai Direct flow of water pe nhi finger pe water girne dete hai phir slide pe Thank you sir ❤

Methanol kitna percent use karte hai sir

Smear dry ayyinaka direct ga stain chesestharu kadha mari fixation enti process lo adhi ledhu kadha

Good experience

Nyc explanation 😊

Thank you sir 😇🙏🏻

But you did not add the buffer!

Super explain sir

Very well explained

Very very Thanks Sir

Thanks 🙏🏻

Sir hm smear 100x pe dekhte hai na 🙄

Bhai methanol me kyun fix karte hai ? Bina uske direct stain kar sakte Hain?

STAINING ROD TO KHARID LE ESR ROD PE LAGA RHA HE STAIN

Distilled water se ku tap water use kr sakte hai

Ek bar ye bataiye ki slide ko kaise dekhenge... Kitne me observe karna hai..... Please tell..

Very nice

Thanks sir

Thanks 🥰

Hai yarrrr krta to haii😂😂 Maanta h ki nhi

TLC count kese kare

You forget to give Cedarwood oil on glass slide.. After that you can view under microscope..

Good

Sir methenol se fix kiu karte hai smear ko

Mstt

Sir what is difference between distill water and normal saline

Nice video

Aap kahan se ho

How to count the dlc smear

Plz determine platelets DLC 🙏🙏🙏🙏🙏 Sir

Thanku sir

Sir ek request hai . Meri slide may black deposit bahut Jyada aate hai.. .agar dobara stain dal kr wash krti hu to platelets nahi dikhte... Plzzz help me Mai kya kru.... Hmari doctor roz bahut Jyada dantti hai Muje... Plz help me 😥😥

Nice one***

Regular video daliye sir ji

Nice sir g

Tomorrow my exam thank god 😅

Mithenal me Dena jaruri hai sir

Don't squeeze the finger tip

GRA kay hai Sir

Great explained thanks sirji🙏

Blood squeeze kar ke nahi nikala jaata h

👍👍👍

But i can not do perfect smear. I also trying 😓😥. I am bmlt 3rd year student

Kitane film dekhegi..?

Blood smear are correct.

Sir apni first drop discharge kyo nhi kyei

Prick sahi nahi kiya

Washing method glt h bhai

Eosin daala he nhi

Sprader ko apna hathome puxlena chahiye

👍🏼👍🏼👍🏼👍🏼👍🏼

Smear is thick

Please wear gloves 🧤

put your gloves on

Apne gloves Nahi pahna, Nd prick k bad 1st blood le liya bo wrng hai Nd slide Nd spreader haat lagana nhi chaiye oil aati hai. Nd labeling v nhi kiya

False procedure.....

Wrong air bubbles nehi hona chahiye

Thanks sir

Thanks 🤗

Good

Sir methenol se fix kiu karte hai smear ko

Thnx sir

Thanks so much sir 👍

Thank you very much sir

Thank you

Lesmain dalne k 2 Min bad buffer solution 2-3 drop dalte h . Fir 5-10 min k bad use tap water se dhona h Uske bad tissue se sukha k use microscope k liye ready krte h

Thanks sir

Thanx sir

Nice

Thank you so much for your effort

Hme edta blood lena hota aa na ki finger prick

Your are wrong don't wash the slide directly in tap water because the stains will washed out and your slide also like that