Generally after the preparation of blood smear, we are not fixing the smear. The Leishman's stain contain methanol. So initially the fixation is obtained by the application of the undiluted leishman's stain (1 min) followed by the addition of the diluent (DW) to stain the cells for 10 mins.
Generally after the preparation of blood smear, we are not fixing the smear. The Leishman's stain contain methanol. So initially the fixation is obtained by the application of the undiluted leishman's stain (1 min) followed by the addition of the diluent (DW) to stain the cells for 10 mins.
