Liquid culture (LC) technique. Expanding liquid culture, inoculating grain, storage, and discussion. 

No, not how it works buddy. No such thing as spontaneous generation. You need spores or colonies, etc, you need contamination physically to be present and alive. Just one spore, or microscopic amount is all it takes. Shaking does not produce contamination magically, if contamination is present in a single colony than shaking will redistribute that contamination making its presence much more known.

@@TacosGaming you took too much. Not sure who you reply to but I did not mention the word "contamination". My LC was not and is not contaminated. I just did not get mycelium out of the jar, only the liquid. This is what I called a failed attempt. I did not mention "spontaneous generation" either. No idea where you got that from. As said, you take way too much to be with "us".

If you go for .5% sugar in recipe shouldn't have caramalisation issues.

Why do you day problems....caramelized lc does the same as mom caramelized....look up videos on the topic. It does not matter

15mins at 15 psi is no problem. This guy is doing a hour cause he has a 12psi pressure cooker. If you have a 13 psi pressure cooker you do 30mins and 15 psi 15 mins.

There are so many autoclaves, and pressure cookers. I'm not sure where to start.

Yeah I agree, the more video Ed makes the more bs info we can all filter out and this guy can show the basics upto the mad scientist end of the spectrum. I learn every video even if it's subject matter I've watched hundreds of times. Keep it up Ed.

It is deprived of oxygen and goes into hibernation/dormancy. The same thing will happen if you keep your plates sealed and they very slowing dry out. I have revived 6 month old plates. As soon as they hit fresh agar, they usually start regrowing.

Never tried. Contamination comes from technique, not ingredients. If the recipe will grow mycelium, it will grow contaminants just as readily.

Yes, they can be PCed, but they tend to distort and become opaque.

Yes. It would work well. You'll just have to figure out the concentration, time of cooking, weights, etc.

I don't know if I'd consider them a replacement for a filter patch, but they work well for me and are easier/cheaper. I think it's about the right ratio of grain to air in the bag. I use about 500g of cooked grain in a 12x18 inch bag. I try to keep the upper half free. You should give it a try if you have access to cheap PP5 bags like I do here in Asia.

I wouldn't reuse them. The PC distorts the barrel and plunger. They are cheap. Not worth the time to reuse.

Yes. Success can be a bit of a burden and a blessing. Start looking for a new house. It only gets worse. :)

They have to be PP% (polypropylene recycle code 5) in order to hold up in the pressure cooker. Any other plastics will melt or get heavily distorted.

Temperature will just affect growth rate. Mine just sits at room temperature. Like 27C.

Not sure the model or manufacturer.

Too little agitation and the myc will run out of oxygen and stagnate/die. Too much and I think it will overgrow and use all the nutrients. The waste products might build up and cause weak grow and recovery. It is a fine balance. There are so many variables to consider to make general guidelines. I usually try to get the myc to optimum density in about 2 weeks. I agitate once or twice a days for about 5 minutes. Once it hits that optimal density, I would use it, pull it and refrigerate it, or just let it sit. If the LC just goes dormant (because of low oxygen), but doesn't die, it will revive when oxygen/agitation is reintroduced or it is put on/in fresh media/LC. If you don't get around to using it or pulling it into syringes (and refrigerating), I'd just let it sit quietly until you're ready to proceed. I have restarted 2 year-old LC that was just sitting quietly at the back of a shelf. As long as it stays clean, no problem.

@@edwardgrand right on, thanks, I do notice that my lc is losing its amber color in those jars - so i suppose its over consumed the nutrients do to my excessive agitation? i would not have thought of that, i was like "damn, i beat that myc up?"

95% denatured ethanol is the fuel. I think it's just called an 'alcohol burner'. You might be thinking Bunsen burner. That is too hot...and expensive. You can DIY an alcohol burner. Check RU-vid 😉

ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-65HTaBhPjLQ.html

Thank you!

I haven't really noticed any degradation after 3 subcultures. I usually clone a good fruit and grow it out on agar, then use that to inoculate a new jar of LC. I also go back to spores frequently.

@@edwardgrand When you say frequently do you mean every year or?

@@moviesandseriesrecap 6 months

@@edwardgrand THX!

I've never heard of anyone purposefully doing it. They do fruit if left alone for months. I am a fan of any grain that is cheap and readily available. I don't think the protein matters. All grains have abundant protein for the production of alkaloids. Mixtures of grains work well, but they need to be prepped separately before going in the PC. I have found corn and rice works very well. Wheat and corn too. But they require very different preps. If I can get one grain cheaply, I just focus on that one and doing it right. That is more important than the nutritional profile. Thank you for the kind words and watching :)

That jar is 500 ml. The liquid is about 150 ml. I like to keep it lower in the jar so it doesn't get near the rim when swirling. That is where contamination will sneak in. All LC recipes are fine. Just don't go past 4% sugar and don't contaminate it :) And don't forget the marbles. LOL

It would be better/easier to make a new container of LC media and inoculate it with a bit of the remaining LC. Using the old bottle and adding stuff to it sounds problematic.

We have an online place called Shopee. Sells everything. Just make sure they match the jars. Many sizes.

Inside the SAB? I guess you wouldn't need to, but alcohol is cheap.

@@edwardgrand thanks for the answer i usually use bleach 1:10 is it good?

You have to make sure the staples only go through the folded parts. I make sure the fold is about 1/4 inch wide and staple as near to the middle as possible. Look closely where the staples are actually going through the bag.

Just operate it as normal. Depending on the volume and type of substrate, you may need to adjust the temperature and time.

@@edwardgrand sir, so can I pasturize my poo substrate at 5psi (75'c) ??

@@yashuu-rk4lc 5 psi will require like 8 hours to sterilize. Don't know what level of sterility you want.

@@edwardgrand nah sir, i want to pasturize my substrate ( killing the bad bacteria and leaving the good bacteria), am not talking abt the sterilization ( killing every micro org)

@yashuu1396 An autoclave is for sterilizing at higher pressures. Pasteurization should be done at atmospheric pressure.

Normal human temperatures. I don't do anything special.

@@edwardgrand so room temp and not refrigerated?

18 gauge is easily available and cheap. Kool-aid doesn't work for LC.

yes

@@edwardgrand ahh ok

@@edwardgrand I’ve been looking online for this answer and I can’t find it so I figured I would ask you! Are purple mystics coir or dung loving mushrooms?

coir@@Houseofmycology

@@edwardgrand sweet I got some from a field and I have been germinating the spores on agar and I have some colonizing on popcorn! But didn’t know if I needed dung or not! Thanks brother

No one would do that.

I'm naked from the waist down...

We are trying to grow a single species. We need to eliminate the competition or replicate the exact conditions that have been fine-tuned by mother nature for 800 million years. Since we've only been around for a tiny blip of that time, we have to make due with our current knowledge of how to encourage them. If you want random mushrooms to pop-up, just let mother nature take over. She does fine by herself.

You obviously don’t have a very good understanding of cultivating mushrooms in a controlled environment if that’s your perspective. In nature, billions of spores are released and only a very tiny percentage of those will germinate and produce mycelium and then grow into mushrooms. In the wild they are competing with millions of other organisms, bacteria and different fungi for a finite amount of resources in any given area. When cultivating at home or in a lab, you sterilize to eliminate as much competition as possible to give your mycelium the best chance of success in ideal conditions . Now, if you want to try the natural way and just throw your spores into some dirt and hope they produce mushrooms without human interference, go ahead and let us know how that works out. There’s a reason everybody talks about sterilization, airflow and temperature . So keep on laughing without an actual understanding of what’s going on. As they say, ignorance is bliss.

These are the types of comments you get when people are ignorant.