Тёмный

Luciferase reporter assay | What is luciferase assay used for? | Applications of Luciferase assay 

Animated biology With arpan
Подписаться 274 тыс.
Просмотров 49 тыс.
50% 1

Опубликовано:

 

2 окт 2024

Поделиться:

Ссылка:

Скачать:

Готовим ссылку...

Добавить в:

Мой плейлист
Посмотреть позже
Комментарии : 76   
@jonashoffmann8225
@jonashoffmann8225 2 года назад
The best Video I've found. Thanks man❤️👍
@maziastro9932
@maziastro9932 Месяц назад
5:47 How would we know whether the promotor is NOT BEING EXPRESSED AT ALL or is a REPRESSOR?
@mamacocolilo105
@mamacocolilo105 2 года назад
So much about this bothers me. Starting with the first 7 letters of this chemical's name. Good presentation though. Thank u
@shawnmulberry774
@shawnmulberry774 2 года назад
It is from the Latin and it means "light bearing". Luciferin is the base protein. The last three letters (-ase) have meaning as well - an enzyme. I was able to synthesize this in my organic chemistry class and it is quite fascinating. It also did not cause anyone any harm. Turn the lights down and you can see the reaction. *Very* useful tool in biology. No need for fear or superstition - it is simply a name that describes what it does. Luciferin is in fireflies - lol and many other things. Sometimes you can see glowing waves of dinoflagellates in tocean waves at night.
@mamacocolilo105
@mamacocolilo105 2 года назад
@@shawnmulberry774 thank u for that! Yes, I was aware of that. I have a degree in a health related field and we touched upon it when i took chem. As beautiful as it is (i believe its also in bioluminescent algae), as a Christian, the name just rubs me the wrong way. Just like the telescope they have in the Vatican. 😉
@shawnmulberry774
@shawnmulberry774 2 года назад
@@mamacocolilo105 If im not mistaken, that particular name does not show up until the King James version. Or at least not in any of the "original" versions of Gospel which would have been circulating before 300AD. Certainly isnt in the original Isaiah. It is really more of a nickname but I do understand your sentiment. Also, the Large Binocular Telescope Near-infrared Spectroscopic Utility with Camera and Integral Field Unit for Extragalactic Research requires a lot of creative choices to get the acronymn LUCIFER. Why isn't it LBTNISUCIFUER? Real astronomers just call it the LBT. This reminds me of NON-scientists calling the Higgs Boson "the God particle". It may be important but come on...
@mamacocolilo105
@mamacocolilo105 2 года назад
@@shawnmulberry774 Agreed. I always thought that science was supposed to be secular. Maybe if the name of the element that was discovered didn't carry such negatively charged name. Like the number 666 what will people think of usually? But people don't know that this used to be code for Nero Caesar (the most evil man of the time). Although its just a number, I've known people who have refused license plates if the numbers are in sequence, or people who refuse to pay a bill (they will ask for a dollar discount or to add another dollar to the amount). Its crazy, but I understand We understandably so take down statues/flags/change the name of places named in honor of historical figures whom other individuals may find offensive or might be sensitive to because of history; and yet when it comes to religion, its not the same (both good or bad, regardless). I wonder why.
@shawnmulberry774
@shawnmulberry774 2 года назад
@@mamacocolilo105 Well, for a long time to be educated, you were probably in the "priest class". My degree is in genetics and probably the most notable founding father of genetics is Gregor Mendel and his famous pea gardening experiments. He was a friar. I think you can be a scientist who is not secular if you have the right attitude. This is not so easy. Even "The Origin of Species" by Charles Darwin is full of homage to how incredible God's creation is. He clearly looked at natural selection and evolution as the way God creates. Often people don't know this because they don't read his work, they read about his work.
@jaimayy
@jaimayy 5 месяцев назад
I'm writing an essay on luciferins in biomedical research, and this video has been incredibly useful! thank you so much!!!
@animatedbiologywitharpan
@animatedbiologywitharpan 5 месяцев назад
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@animatedbiologywitharpan
@animatedbiologywitharpan 5 месяцев назад
don't forget to subscribe
@jaimayy
@jaimayy 5 месяцев назад
@@animatedbiologywitharpan of course!
@InnovativeSaint
@InnovativeSaint Месяц назад
I know Lucifer means light, and it would seem many advances in medicine have a Latin predisposition, nevertheless, Lucifer race (how it sounds) seems quite interesting.
@haiyen0325
@haiyen0325 4 месяца назад
Thank you
@animatedbiologywitharpan
@animatedbiologywitharpan 4 месяца назад
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@haiyen0325
@haiyen0325 4 месяца назад
@@animatedbiologywitharpan sure
@sny8317
@sny8317 7 месяцев назад
Why it’s in a vector ? Why they trying to mess with human genomes ?
@animatedbiologywitharpan
@animatedbiologywitharpan 7 месяцев назад
This has nothing to do with a human . It's to test biological principles
@Hazendal777
@Hazendal777 5 месяцев назад
to makes us slaves of the high " elite " 😢
@sny8317
@sny8317 11 дней назад
@@animatedbiologywitharpan I got the fact sheet of the ingredients of the C shot when it first introduced and I saw this substance in it ….
@guihuajia7696
@guihuajia7696 Год назад
very useful!
@animatedbiologywitharpan
@animatedbiologywitharpan Год назад
Please share my channel link with your friends and help me to reach big audiance. namaste 🙏
@guihuajia7696
@guihuajia7696 Год назад
Hello, could you please let me know how to produce dsRNA?
@animatedbiologywitharpan
@animatedbiologywitharpan Год назад
You mean cDNA ?
@guihuajia7696
@guihuajia7696 Год назад
@@animatedbiologywitharpan Sorry, I don't know what was it exactly, but I refferred was "dsRNA" that makes target gene silence. Do you always produce cDNA to generate the so-callled dsRNA?
@animatedbiologywitharpan
@animatedbiologywitharpan Год назад
@@guihuajia7696 may be you are talking about RNAi
@guihuajia7696
@guihuajia7696 Год назад
@@animatedbiologywitharpan Yes sir, i were talking about it. Thank you.
@muditgupta5968
@muditgupta5968 2 года назад
Hi, Thanks Arpan, wonderfully explained!!!
@animatedbiologywitharpan
@animatedbiologywitharpan 2 года назад
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Small contribution like Rs50/100 means a lot for us.
@percypercy4810
@percypercy4810 Год назад
Thank you so much. This video was extremely helpful
@animatedbiologywitharpan
@animatedbiologywitharpan Год назад
Please support my channel via super thanks. Your small contribution like 1$ means a lot for us. The super thanks option is present at the lower right corner of any video, a heart sign with $ in it.
@sippakorn1410
@sippakorn1410 24 дня назад
Thank you for your useful video
@animatedbiologywitharpan
@animatedbiologywitharpan 24 дня назад
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@JaceTan-90
@JaceTan-90 3 месяца назад
Clear, visually simple to digest and easy to recall! Many thanks.
@animatedbiologywitharpan
@animatedbiologywitharpan 3 месяца назад
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@AnnieO100
@AnnieO100 Год назад
It’s in the jab.
@juthibiswas94
@juthibiswas94 Год назад
Nice explanation. Could you please make videos on TF profiling?
@animatedbiologywitharpan
@animatedbiologywitharpan Год назад
Please elaborate what you exactly mean about TF profiling
@星胡-r4q
@星胡-r4q Год назад
hello thanks for shareing this video to explain the assay!my question is where renilla internal control gene insert to the gene of interested?tyvm
@animatedbiologywitharpan
@animatedbiologywitharpan Год назад
Various plasmids encoding Renilla luciferase under different promoter constructs are commercially available. The validity of the use of Renilla luciferase as an internal control is based on the assumption that it is constitutively expressed in transfected cells and that its constitutive expression is not modulated by experimental factors that could result in either the upregulation or the downregulation of the amounts of the enzyme produced. During the past ten years, a number of reports have appeared that identified a variety of conditions that could alter the basal constitutive expression of Renilla luciferase.
@amruthalakshmi2762
@amruthalakshmi2762 2 года назад
Nice video and explained very well
@animatedbiologywitharpan
@animatedbiologywitharpan 2 года назад
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
@amruthalakshmi2762
@amruthalakshmi2762 2 года назад
Yes sir sure
@bizhenyu
@bizhenyu 6 месяцев назад
This explanation is really good, thank you!n
@animatedbiologywitharpan
@animatedbiologywitharpan 6 месяцев назад
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@KeithMakank3
@KeithMakank3 3 месяца назад
THe power of christ compelss you!!
@animatedbiologywitharpan
@animatedbiologywitharpan 3 месяца назад
ok
@soundaryamohan6406
@soundaryamohan6406 Год назад
Excellent explanation
@animatedbiologywitharpan
@animatedbiologywitharpan Год назад
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
@MsMarkopolo123
@MsMarkopolo123 2 года назад
It literally says lucif erase
@MsMarkopolo123
@MsMarkopolo123 2 года назад
What is lucif erasing
@aqua6613
@aqua6613 Год назад
@@MsMarkopolo123 people who serve Christ
@IamFreefromtheWokeLeft
@IamFreefromtheWokeLeft 4 месяца назад
@@MsMarkopolo123 DNA
@Анжелика-к9э
@Анжелика-к9э 5 месяцев назад
thank you so much from Russia
@animatedbiologywitharpan
@animatedbiologywitharpan 5 месяцев назад
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@luismatheu4226
@luismatheu4226 Год назад
Great vídeo thanks
@animatedbiologywitharpan
@animatedbiologywitharpan Год назад
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
@animatedbiologywitharpan
@animatedbiologywitharpan Год назад
Don't forget to checkout my playlist
@njm41
@njm41 2 года назад
Thank you sir.
@animatedbiologywitharpan
@animatedbiologywitharpan 2 года назад
Really glad to know it was useful. For producing high quality videos my channel needs your support.You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
@starzwoo
@starzwoo 2 года назад
thank you!
@animatedbiologywitharpan
@animatedbiologywitharpan 2 года назад
Watch my video on brain facts official cahnnel and don't forget to like. link ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-gCSKpNdJkZo.html
@julia-ho9yg
@julia-ho9yg 7 месяцев назад
omg this explanation was so good, thank you!
@animatedbiologywitharpan
@animatedbiologywitharpan 7 месяцев назад
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@AmruMagdy
@AmruMagdy 10 месяцев назад
images (2) ببب«О Боже, позволь нам достичь Рамадана, достижения, которое изменит нашу ситуацию к лучшему, очистит наши души и наши внутренности, достижения милосердия, прощения и свободы от ада».
@sny8317
@sny8317 7 месяцев назад
Jesus is God
@mitchell186199
@mitchell186199 11 месяцев назад
Excellent video!
@animatedbiologywitharpan
@animatedbiologywitharpan 11 месяцев назад
Please share my channel link with your friends and help me to reach big audiance namaste 🙏
Далее
Pull-Down Assay Protocol
11:58
Просмотров 34 тыс.
У КОТЯТ ОТКРЫЛИСЬ ГЛАЗКИ#cat
00:26
Iran launches wave of missiles at Israel
00:43
Просмотров 1,2 млн
A Crash Course on Luciferase Assays
9:41
Просмотров 10 тыс.
What is Epigenetics? - with Nessa Carey
39:26
Просмотров 367 тыс.
Your Unstoppable Copy Machine|DNA Replication
15:21
Luciferase Reporters in Circadian Research
7:53
Просмотров 51 тыс.
What is life and how does it work? - with Philip Ball
51:51
У КОТЯТ ОТКРЫЛИСЬ ГЛАЗКИ#cat
00:26