Making a 3-5% RBC Suspension

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PROPERTY OF THE INDIAN IMMUNOHEMATOLOGY INITIATIVE
www.indianinitiative.org
Susan Johnson, MSTM, MT(ASCP)SBB, MS, Associate Director of the Indian Immunohematology Initiative, demonstrates how to prepare a 3-5% red blood cell suspension that can be used in hemagglutination testing to determine blood group.
www.indianinitiative.org
Доступно с русскими субтитрами! Приготовление 3-5% клеточной суспензии.

Опубликовано:

19 май 2013

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Комментарии : 16

@Globetrotter_Traveillii 9 лет назад

another method wash cells 3x, then on other tube put for example 500 microliters of saline then computed 5% is 500 x .05 where 500 is saline and .05 is 5% pure washed red cells so 25 microliters =5% red cell suspension.

@mahmoudtaha8879 4 года назад

Excellent. Very helpful.

@TheJessicarabbit09 Год назад

Super helpful, thank you!

@iloveegypt1324 9 лет назад

many thanks ...

@mehrdadfathi941 4 года назад

Thank you very much

@PhillipH-san 6 лет назад

Thank you very much!! That's such a good tip to compare your suspension to the reagent cells! Brilliant! Haha All of my classmates will be elated when I relay this technique to them.

@yasminali7578 6 лет назад

excellent

@TheFreealexis 8 лет назад

I usually like to compare the suspension to the color of fruit punch. I have my lab practical tomorrow and this was a very helpful video.

@oylezabell2603 8 лет назад

hello i have a questions 1. Why need to have 5% cell suspension? 2. What is the purpose 3. Different between use whole blood and 5% cells suspension

@nabayuot 10 лет назад

what if commercially prepared cell suspension is not available to compare with?

@nanabearnguyen 7 лет назад

you need you buy one, then you can make one to keep it available for the next suspension

@4cgasmenjanleo478 3 года назад

why is it called 3-5%??

@greeniris9290 7 лет назад

im sorry i didn't understand well but why is there are 3 pairs of tube?

@PhillipH-san 6 лет назад

Green Iris I believe there are a total of 3 different concentrations (just right, too heavy, and too light). Each suspension was tested with both anti-A (blue reagent) and anti-B (yellow reagent).

@PhillipH-san 6 лет назад

If this was a real patient workup, there would only be 2 tubes for the forward type.

@mahmoudtaha8879 4 года назад

it is for demonstration only to know what will appear if we use too diluted or too concentrated RBCs suspension