You can't just grind cat-litter tier silica gel and expect it to work for column chromatography. Chromatography grade silica gel is mesoporous, with a high surface area. It is made from waterglass and a surfactant like sodium lauryl sulfate, first precipitating the silica, drying at 110°C, then calcifying at 400°C. Then you have to sieve them and only keep the fraction between 40 and 63 microns for column and flash chromatography. Mesoporous silica gel is used in beer brewing however, to clear the haze without affecting foam, by selectively adsorbing proteins. You can use this kind of silica for chromatography purposes, but it is probably too finely divided to use in a column, it will take ages for the solvent to run through. It"s more suitable for making your own TLC plates.
Worst attempts at column chromatography.. ever 😂😂😂 Take ur time and think before doing so u dont waste time overall, but too much thinking can be detrimental lol Cool content regardless!!
Sorry mate, but you have not only 1 mg of cubane there. If you take a look at the dexter-electron transfer, then you can easily see, that O2 is a problem there. The energy will be transferred to the O2 instead of the product and that's your problem. You need an inert gas without any mg of O2. I have done this mistake three times. So sorry, but you have to run it again.
Then why does the brown precipitate react with acids and bases like that? I ran the UV reaction for so long that even through there was oxygen present some of the diketal must have reacted.
@@Amateur.Chemistry and no, I guess not, because the Cycloaddition doesn't take so long. If you have O2, the triplet sensitizer benzophenone will just transfer the energy from the excited state to the oxygen, not to the diketone. And the products have to be pretty clean for the cycloaddition.
