Admittedly, i have trouble understanding how the ion filter that separates the different ion packages works. How can separate ion packages be included/excluded when they travel at the same speed as the rest of the ionized matrix?
Dear Michel, thanks for your question. The ion filter (precursor ion selector) works via timing. From the acceleration to the precursor ion selector is the first time of flight distance so different precursor masses with their respective fragments separate from other masses, so the precursors (let us assume m/z 1000) travels at the same speed as their fragments in the field free region of the first ToF. But other ions (e.g. m/z 1100 and their respective fragments) travel at a different speed (due to the initial acceleration energy applied). As we know the distance and energy being applied we only have to allow a given precursor mass (with its fragments) to pass at a preset time and then closing the precursor ion selector again for all other masses. By accelerating the selected precursor ion and its fragments after the precursor ion selector, the fragments are then separated from the precursor in the second TOF. Kind regards your Bruker Marketing Team
Hi Stefunny, thanks for your question. TOF stands for “Time Of Flight”. TOF TOF means “Tandem Time Of Flight”. Both Mass Spectrometry concepts are similar but different during the measurement of your sample. MALDI TOF makes it possible to determine molecular masses while MALDI TOF-TOF provides you an deeper analytical insight of targeted compounds.
Hi and thanks for the video. I am quite curious about the application pros and cons between QTOF and TOF/TOF. I understood their difference in terms of mechanism (Quadrupole as mass filter vs ToF + timed ion gate as mass filter) but I am having a hard time finding which one is "better". Furthermore, I noticed that QTOF are predominantly coupled with ESI while ToF/ToF almost exclusively with MALDI. Is there a reason behind this?
I am not a Bruker employee but I work for my PhD in the field of mass spectrometry, so let me try to answer your questions. qToFs are typically coupled with ESI (or other API sources) because the ToF measurement requires a defined starting point. In case of MALDI, this starting point is defined by each laser pulse and, hence, MALDI is directly coupled with ToF analyzers. In case of ESI, the starting point is defined by an orthogonal accelerator (oaToF) that accelerates the ions orthogonal to their original flight path. This oaToF set-up allows for coupling with quadrupoles as the orthogonal accelerator requires low ion speeds, which are also required for quadrupoles. The MALDI ToF coupling, on the other hand, provides extremely fast moving ions which prevents the usage of quadrupoles in these devices. Answering the question about dis/advantages of qToF vs ToF/ToF is hard because both techniques are well suited for acquiring MS/MS spectra. Overall, its rather a question of which ionization technique is more suitable for your samples. However, I personally prefer the qToF set-up for MS/MS experiments because quadrupole collision cells allow for measurements at well defined collision energies which are really important for my research.
Hi chachouromynews , in this Video we are showing our "rapifleX". This Instrument has a size of 95 x 80 x 275 cm. Of course we also have smaller Bench Top Instruments, like our Microflex Series.
sorry to be so off topic but does someone know of a trick to get back into an instagram account..? I was dumb lost the account password. I love any help you can give me
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