Maxam gilbert DNA sequencing method

Подписаться 2,1 млн

Просмотров 396 тыс.

50% 1

This DNA sequencing lecture explains about the Maxam gilbert method of DNA sequencing or chemical DNA sequencing.
For more information, log on to-
www.shomusbiology.com/
Get Shomu's Biology DVD set here-
www.shomusbiology.com/dvd-store/
Download the study materials here-
shomusbiology.com/bio-material...
Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in RU-vid. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology-
Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store
Shomu’s Biology assignment services - www.shomusbiology.com/assignment -help
Join Online coaching for CSIR NET exam - www.shomusbiology.com/net-coaching
We are social. Find us on different sites here-
Our Website - www.shomusbiology.com
Facebook page- / shomusbiology
Twitter - / shomusbiology
SlideShare- www.slideshare.net/shomusbiology
Google plus- plus.google.com/1136485849827...
LinkedIn - / suman-bhattacharjee-2a...
RU-vid- / thefunsuman
Thank you for watching

Опубликовано:

22 мар 2015

Ссылка:

Скачать:

Готовим ссылку...

Добавить в:

Мой плейлист

Посмотреть позже

Комментарии : 263

@praveenvemuri 7 лет назад

Its a good try, but there are few things which has to be dealt for better understanding like: 1. what chemicals are used specifically to cleave a certain nucleotide (G-DMS, A-Formic acid, C-Hydrazine+NaCl, T-Hydrazine) together with piperdine to cleave the phosphodiester bond of modified nucleotide 2. How to make single stranded DNA fragments (heat denaturation or using helicase) 3. Dimer formation are in combinations of A+G and C+T If a band in the DNA sequence appears in both the G-reaction and the G+A-reaction lanes, then that the nucleotide is a G. If a band in the DNA sequence appears only in the G+A-reaction lane, then it is an A. The same decision process works for the C-reaction and the C+T-reaction lanes. 4. What quantity of sample DNA is required for sequencing (if not sufficient, have to run PCR or do cloning to amplify) 5. What about the left part of DNA strand after cleavage (they cant be visualized, due to lack of isotope) 6. What percentage of gel is preferred (6% polyacrylamide gels are used, bcz they are too sensitive in differentiating the DNA bands with 1bp size)

@ProfSardarMNiaz 7 лет назад

Praveen Kumar you are stunning.help me a lot

@savitapal9943 7 лет назад

hello friends

@rishabhjain7690 6 лет назад

Praveen Kumar Thanks

@roshanaatreyapaudel4033 5 лет назад

Thanks

@yingliweng9759 5 лет назад

thanks

@mpv6426 6 лет назад

I just realised that I've been passing my entire uni thanks to you but never said a proper thank you so: Thank you! (=

@Sheikhandbirds 3 года назад

Same 😂😂😂

@rosh_serendipity939 Год назад

Samee 🥹

@postmorton2493 3 года назад

I'm doing an essay on genomic analysis, and these videos on sequencing are amazing for a proper, scientific understanding of how sequencing has developed over the years. Thank you so much for the clear and helpful video!

@shomusbiologyofficial 3 года назад

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

@ananyayadav9686 5 лет назад

I came across this video , and may i say you are the best youtuber teacher we have got Hands down sir, you are brilliant

@romi6374 8 лет назад

seriously... The Best Lecturer Out there.. I find it so effective.

@AzhunkWorld 6 лет назад

Interesting and very clear explanation. don't bother about the time because it's very interesting and understandable. Thanks shomu!

@rafaelkyranas3131 8 лет назад

Dude you are the BEST! KEEP IT UP

@fatimariaz1233 6 лет назад

when you write down on board that method is good for understanding and due to only this difference between you and other you tubers i was watching your videos

@alwinaanam1231 7 лет назад

indeed its helpful...........complicated topic bt after watching twice /thrice i can understand for sure..

@shomusbiologyofficial 7 лет назад

+Alwina Anam thank you very much

@RafaelMazenett 9 лет назад

Awesome. It helped me a looooot. THANK YOU

@RosemaryUkamaka-ie7uc Год назад

This is so helpful but I almost got confused at the initial result interpretation but thank God, I later understood it. Thank you Shomu Biology

@shomusbiologyofficial Год назад

You're welcome

@sohailvet14 4 года назад

I am a veterinary student .....GADVASU....number one veterinary university in india......still we dont have any teacher like u sir......thank u very much❤️❣️

@shomusbiologyofficial 4 года назад

Glad to hear that you're getting benefit from my lectures

@chandrat1221 2 года назад

Thanks! what is the sequencing QiaSeq target amplicon method different from Sanger sequencing method ? which is superior?

@reshmirajeev5770 3 года назад

Thankyou sir.....you re the best teacher...😍

@hadogori4613 3 года назад

I love your explanation♥️ Thank you so much ♥️

@ejazkhan2952 7 лет назад

no need for short videos....you are doing great job... because it is helpul for begginers,,,if you start to make short videos ...then the beginners will not understand....thanks alot...very helpul.....

@shomusbiologyofficial 7 лет назад

+Ejaz Khan thank you.

@bamd9 5 лет назад

but what about the first nucleotide ? how to know it?

@nzolelejuma8167 6 лет назад

Sorry sir can you tell me a little beat in interpretation of result sequence if you have C and G of the same size what base will you labble first

@marwahussien718 6 лет назад

thank's very much now I depend on you very much to understand any thing .I wish I Can reply the favour ❤❤ and please don't stop many students depend on you . Marwa from egypt

@ayanasudheer2934 5 лет назад

How did you know that the very first base in the gel is Adenine?? Someone pls answer

@wassimsouissi8330 8 лет назад

Does each reaction require many single-strand DNAs ? ( cause there is too many fragments )

@aboutstudies123 6 лет назад

Thank you very much Sir. It helped me a lot

@rumibhadoria4009 4 года назад

Sir your channel is v helpful . I get everything here

@CringeDealer69 7 лет назад

Really really helpful... Understood the topic thoroughly... ☺️

@shomusbiologyofficial 7 лет назад

+mayur tupe thank you. Glad you liked my lectures

@CringeDealer69 7 лет назад

Shomu's Biology My pleasure... ☺️

@AaRTiSharma-qs7em 7 лет назад

simply awesome!! got it :)

@jaydeepdumadiya2464 3 года назад

Beautiful sir... God bless you.. 🙏🙏🙏🙏🙏🙏🙏

@nabeelkhan-yh9rx 6 лет назад

sir carry on your vedios are very helpful

@KountayDwivedi 3 года назад

Awesomely explained. This is my first video on Maxam-Gilbert, n you cleared the concept seamlessly. I won't go into technical details, I will just say that concept-wise this lecture is awesome. :-}

@shomusbiologyofficial 3 года назад

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

@anjiman5890 3 года назад

@@shomusbiologyofficial,your class is really helpful, sir you take an example of 12 nucleotide, and in autoradiography in the 11th portion the 12th nucleotide come..can you please explain it

@SedighehMonavari 7 лет назад

great explanation. thanks :)

@divyangdamor7380 9 лет назад

thank you... great explanation...

@oubaidaelbiad6486 7 лет назад

doing a great job man (Y)

@jerry-pc6fl 6 лет назад

but how can we get the first base by using this method

@aimalkhan385 7 лет назад

infact it is helpful. Great.

@udehedith7326 5 лет назад

The video was quite helpful and made understanding easier. Thank you very much

@shomusbiologyofficial 5 лет назад

You're welcome

@mssazeb 7 лет назад

i have a question.. why we select G and C bases separately.. i mean we took purines and pyrimidines in two test tubes n G and C in rest of the two so why not A and T??? and we treated T+C tube with the chemical to cleave T and upto some extent C as well.. so why that chemical isn't working for T?? n why the result is same for C and T+C???? reply

@pratitidas 6 лет назад

Can u give the link of the biochemistry book

@arshiafsana472 6 лет назад

And what about first base Adenine...gel doesn't have this info.. so whats the reason sir..plzz...tell us

@tamannaparvin5797 2 года назад

Thanks for this video. It's really helpful for me.....This topic is in my syllabus....thanks a lot

@shomusbiologyofficial 2 года назад

You're welcome

@umairameer6728 3 месяца назад

How can you identify A at Terminal 5 prime end. As it shows the band in T+C.

@nemapusai 2 года назад

Very nice sir ! no words to tell and your classes helped a lot. thank you soo much sir

@shomusbiologyofficial 2 года назад

Thank you so much for appreciating my efforts

@taniagoyal3523 4 года назад

Won't their be be combinations for cleaving out T in 3rd tube Like 1 and 2 T And another one 1 and 3 , 1 and 4, 2and 3, 2 and 4?

@drsahivlogs676 3 года назад

My best BIOLOGY teacher over whole youtube

@shomusbiologyofficial 3 года назад

Thank you so much for appreciating my efforts

@drsahivlogs676 3 года назад

@@shomusbiologyofficial Love From Pakistan

@lanoanhduongthi3536 8 лет назад

thanks for your explain

@md.shaminurrahman8758 6 лет назад

You are the best💜💜💜

@bhagyajitdas1498 5 лет назад

Thank you sir for explaining it beautifully!

@shomusbiologyofficial 5 лет назад

You're welcome

@sushantikasubbiah1931 5 лет назад

During chemical termination is the single strand added newly to each test tube everytime?

@swatipatil9156 3 года назад

Bamh1 recognise 6 base pair of DNA in palindromic sequence if the first part sequence is given below what is rest of it? 5 C A A3

@kavitachoudhary6168 3 года назад

Sir I have a question As we can see that while chemical degradation sequence is cleaved at the site just before to that perticular residue so what about A which is radiolabled as a first residue. How we will know that A is present at first becs when it get cleaved we will get only phosphate without sequence?

@nohabassiouny6849 5 лет назад

you have a simple way in teaching and presenting the idea

@shomusbiologyofficial 5 лет назад

Thank you. Glad you liked my lectures

@anjalisharma7394 3 года назад

It’s very clear nd helpful .thank you for this🙏🏻

@shomusbiologyofficial 3 года назад

You're welcome

@mhjahid01 6 лет назад

Thank you so much SIR

@techforever1970 5 лет назад

Thank you so much! You helped me alot. I owe you.

@shomusbiologyofficial 5 лет назад

Thank you. Glad you liked my lectures

@khalidmehsud54 3 года назад

Hain?

@top-uptv7010 Год назад

Please what is the name of the referenced book, how do we get it

@afnansalah4031 7 лет назад

you are AMAZING

@anasahmad9145 6 лет назад

Great work

@anasahmad9145 6 лет назад

Awsome great work love you

@sheikhabrar9932 6 лет назад

praveen Kumar.....thanks for adding more information

@anjalisetia8672 6 лет назад

why the first base (A) is not identified..?

@kundandutta710 3 года назад

Sir why we Have To elute out the heavier band and work with only the lighter band after the denaturation of DNA double-strand?? Please reply sir

@subhamsaha2235 7 лет назад

waah sir...lovely...very easy

@princecudjoe5518 Год назад

that was some top explanation man. thamks!

@shomusbiologyofficial Год назад

You're welcome

@muskaankhalifa5059 Год назад

Sir, which book to refer for this?

@DiegoDiego1989 6 лет назад

I love this guy

@supriyohalder7889 6 лет назад

thank you bro i got help from this video.. keep it up

@shomusbiologyofficial 6 лет назад

Thank you

@basavarajgonwar7723 6 лет назад

Thank u very much

@adarsh7221 6 лет назад

How can you tell the first nucleotide is A(adenine) from electrophoresis? Please answer

@asmasbiology3 3 года назад

yes plz

@rupalichakraborty4814 6 лет назад

Thank you sir

@nusheratpervez8868 6 лет назад

Thanks alot👌👍

@aakaankshavangala1826 5 лет назад

1. Not Chemical Synthesis. It is Chemical Cleavage (two completely opposite meanings) 2. First nucleotide determination without which DNA sequencing is incomplete. From my understanding, you are supposed to initially cut/cleave the DNA sample with a restriction enzyme. From this you get to know where the DNA is getting cleaved (as you know the restriction sequence of the enzyme). This is then followed by the steps explained in the video.

@sukhmanpreetkaur7951 4 года назад

can u plz refer me any book for this topic

@Serwada237 3 месяца назад

Better than my class work ❤🎉

@shomusbiologyofficial 3 месяца назад

You're welcome

@Serwada237 2 месяца назад

Iam so happy that you replied to me I have used your site to undertand concepts for my molecular biology masters degree. At Makerere university Uganda… thank you we appreciate your efforts to teach the world science

@partha_plethorapedia 5 лет назад

Thank you sir....

@swetapanwar6993 3 года назад

Sorry sir...i m not able to getting this in last...how we do count ...if we don't know the sequence of complementary then how we count the Nucleotide bases....i saw this video repeatedly but i didn't get it after electrophoresis ..... please tell

@pankajpulate8961 7 лет назад

THANKS U SIR TO INFORMATIVE

@jannatulferdous9390 7 лет назад

Thanks a lot.

@imamanoor5867 8 лет назад

thank u so much!!!!!!!

@scienceacademybywaliafridi3528 3 года назад

Great job sir

@magnfiyerlmoro3301 3 года назад

what do you mean by homogenous dna please thanks

@manishmehra9748 8 месяцев назад

sir why are we unable to find the first nucleotide ?

@monicasingh4156 6 лет назад

It's really helpful...

@shomusbiologyofficial 6 лет назад

Thank you. Glad you liked my lectures

@dr.jyotisaxena6966 4 года назад

Like many others I also want to know whether 1st base can be detected correctly from this method or not.......if not why

@supritadash2535 7 лет назад

Thank u so much.. really helpful

@shomusbiologyofficial 7 лет назад

+Suprita Dash Glad it helped

@nothingggg656 9 месяцев назад

Thankyou for the wonderful explanation sir❤❤

@shomusbiologyofficial 9 месяцев назад

You're welcome

@darshan00185 3 года назад

Sir it was very helpful but how A is the 1st nucleotide is not I think explained

@malleshdurgam568 2 года назад

Thank you very much sir... 🙏

@shomusbiologyofficial 2 года назад

You're welcome

@maitypartha Месяц назад

How can find tha 3'A seq then ?

@tunerd1921 3 года назад

What was the book called?

@ankitarout2688 2 года назад

It's really helpful.. Thank you so much

@shomusbiologyofficial 2 года назад

You're welcome

@prempratyushmangraj1050 11 месяцев назад

Sir.. How can we know about the first nucleotide?

@kushaniakshala3758 3 месяца назад

Great...... Thank you

@shomusbiologyofficial 3 месяца назад

You're welcome

@shreyarastogi4925 4 года назад

Can chemical sequencing be used for RNA sequence?

@riichobamin7612 5 лет назад

In the results interpretation part, you appear to be removing even terminal nucleotides (like you removed the C from the T+C tube). Didn't you say that the terminal nucleotides cannot be removed ? Also in what manner are the nucleotides being fragmented ? There should be atleast 4 different types of fragments in the G tube for example if we remove the G from the middle.

@samjhanastha6647 7 лет назад

extremely helpful!!!

@shomusbiologyofficial 7 лет назад

+samjhana stha thank you. Glad you liked my lectures

@edeeaminajoy6155 4 года назад

Where can I get the ppt file?

@duraiswamy8027 3 года назад

At 1 guanine reaction ,u told that DMS will cleave the guanine ,so no stretch of DNA will form . But how it produce 3 types of strand at guanine reaction ,because DMS will break sequence ,so we will get same type stretch DNA only sir ,???