This video describes the purpose, procedure, and result interpretation of the qualitative & semi-quantitative Non-Treponemal Screening (RPR) test for Reagin type antibodies. A comparison of this and the VDRL test is also included.
Can post more videos like this on every test in the laboratory because im an intern and i dont have experiences during the pandemic and our school doesnt allow us to go to the hospital because of the virus
I have been looking at multiple images of card test like what you have in this video. How can I develop my ability to identify a positive on a patient barely showing a faint discoloration from reagen with a titer of 1:1? Images I have seen to have ratios of >1:8 would equal reactions quantified as 1:512. Several members of our lab are having extreme difficulty distinguishing positives such as this. I’m beginning to wonder if it’s realistic based on images I have seen available are very distinctive reactions which is not what we are seeing.
Good question! There is more of a separation within the liquid itself when the test is positive. The aggregates end up spreading along the periphery of the test window to make a large ring. Negative reactions tend to stay as a "fluid blob" or drop that mostly stays together as it rolls/flows along in the test window. You may see the particles of the reagent but that is not a true positive.
Please help. So I tested positive with rpr weak reaction titer 1;1.. Then did another test TPPA that one was non reactive. What should I make of these results? Thank you. Btw I am a male.
According to my microbiology books, RPR is usually positive for Borrelia burgdorferi and other bacteria, not only T. pallidum. About Acinectobacter, I don´t know sorry.
That looks like a straight solution to me...just specimen. If there is a dilution, the person might have meant 1:2 (1 part specimen in 2 parts solution with an equal part being diluent).
