Microscope Slide Prep: Observing Spores and Finding Clamp Connections

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**Disclaimer** Because of the nature of microscopy and this video, any claims are pure speculation. Multiple slides were used and no confirmatory testing was performed. This highlights the procedures used.***
This video breaks down a few different procedures for preparing slides for microscopy in mycology. Three common methods: Wet Prep, Heat Fixing and Scotch Tape Prep are used to observe spores and mycelium under the microscope. Enjoy and MUSHLOVE!
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2 фев 2023

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Комментарии : 37

@mycomomjeans Год назад

Thank you so much for this series. I took microbiology 20yrs ago and your microscopy series has been so helpful in refreshing and expanding my knowledge! Your work and content is much appreciated ! 🍄❤️

@FreshfromtheFarmFungi Год назад

thanks for watching and following along!

@johnharrison6009 Год назад

Hey Gary, Thank you so much! Again, it is such a pleasure watching a real pro demonstrating their expertise and proper techniques. Time to dig out the old microscope! Thanks again!

@tonystewart7624 Год назад

Thank you for sharing this video. I got a microscope a few months ago and it's been sitting idle. Now I'll feel more confident using it.

@avk-pg5rb Год назад

This was great! Thank you for this video and the many more you have uploaded regarding breeding, genetics, and the scientific explanations you upload. Love all the videos and THANK YOU!

@sporgymushrooms9656 Год назад

Gary, you da man! Thanks for the clamp connect and slide prep demo. Looking forward to those new cultures I picked up on your Etsy shop!

@FreshfromtheFarmFungi Год назад

thanks! yes hope they do well for you! HAPPY GROWING AND MUSHLOVE

@RustyColonsCPA Год назад

Great info as always! Mushlove!

@stacibryant3353 Год назад

Much needed information. Thanks for sharing!!

@mycologylids Год назад

Awesome content as always.

@MissBlackMetal 2 месяца назад

15:00 and 17:00 examples of clamp connections (when 2 hyphae mate and exchange DNA)

@davidcollett1847 Год назад

Thanks man just getting in to 🔬 just ordered one today

@davedivesdeeper Год назад

Mush love!

@tims-pt1yy 11 месяцев назад

This topic screams for more 😁

@saabitrishrestha2811 Месяц назад

Thank you for this video. Could you also please make a video regarding the Basidiospore size, expressed as (ℓext.min)ℓmin-ℓmax(ℓext.max) × (wext.min)wmin-wmax(wext.max), and the average size as ℓM × wM, where “ext.min” .

@momsmushroomsjodyfoster5786 Год назад

Let me know if you should start offering classes. I'm especially interested in breeding cordyceps

@coloradodirtbike5930 Год назад

Too cool 😎

@InconspicuousOwl Год назад

Nice.

@momsmushroomsjodyfoster5786 Год назад

You've got an awesome variety of stuff on Etsy. I got the camo hat for my husband for Christmas. I had a random question. If I want mushrooms ready by the 1st of May when should I start it. I grow from spore or L.C. not kits..also can you suggest what ones do well in the tent together. What name some that grow under the same conditions plz. Thx Gary

@FreshfromtheFarmFungi Год назад

it usually takes about 2 weeks at each phase (2 weeks on agar, 2 weeks on grain, 2 weeks on bulk and 2 weeks to fruit) That being said, there can be setbacks with contamination or some will take much longer (shiitake for instance). Most mushrooms will grow well in the same tent around 65F

@user-oo4vi5iv3d Год назад

Haw can I prepare bromothymol blue please? I have bromothymol blue powder

@phillymontana Год назад

Thanks for the video Funguy. Quick question - what would be the benefit of looking at spores or mycelium through the microscope? Is there any information I could gather from that process? I've got a microscope and was wondering if I could improve my mycology by doing this exercise somehow.

@FreshfromtheFarmFungi Год назад

for breeding it helps find mated pairings by looking for clamp connections- it also helps to identify unknown mushrooms or unique strains because of spore characteristics

@PhilScottCanada Год назад

@@FreshfromtheFarmFungi Please do a video on spore characteristics.I want to start using my microscope for this. Thx.

@tonystewart7624 Год назад

I don't know if you noticed but it looked like there was movement to the left of the last mycelium clamp connection displayed. Cool beans

@FreshfromtheFarmFungi Год назад

that looks incredible looking back on it thanks!

@tonystewart7624 Год назад

@@FreshfromtheFarmFungi I thought so too! You're most welcome. I hope you have fun exploring what it is.

@marvin216 Год назад

Where do I find the video on how to isolate a single spore using a Pipette tip?

@FreshfromtheFarmFungi Год назад

It’s going to drop this weekend 🙏🏻

@user-oo4vi5iv3d Год назад

What is the Name of the stain??

@FreshfromtheFarmFungi Год назад

bromothymol blue you can also use lactophenol cotton blue

@user-oo4vi5iv3d Год назад

Haw can i prepare bromothymol blue please? I have the powder

@themyceliumnetwork Год назад

can I borrow your compound microscope ? I'll bring it right back when I'm done.......

@kidorhymes Год назад

How about to get proper microscope more suitable for mycology ? :) Like old inverted Olympus CK with proper lenses, then u will be able to observe and work with everything on agar, event single spore isolation as u got plenty of space from above... Proper lenses can focus trough agar with no problem... we use them daily here and it's like light years away from classic ones

@FreshfromtheFarmFungi Год назад

ah yes for the next series I can upgrade. These Amacopes are less than 200$ the Olympus start around 5k. I agree that they are better, but for an at-home mycologist that capital could go towards many other things while this scope works well enough (the “Honda civic” of microscopes as I have stated earlier) Olympia is like a Cadillac - thanks for the feedback and next time you bust that scope out hopefully you will thank the mushroom gods and have some gratitude 🙏🏻 MUSHLOVE 🍄❤️

@kidorhymes Год назад

@@FreshfromtheFarmFungi Maybe some used one from ebay ? :) My was around 500 $ Basic series of new ones are around 1700. but yes for sure they are much more expensive, but also much more suitable for the job :) And if u will get one with micro manipulators u can forget on serial dilution as is really easy to pick spores or anything else :)