I checked every website that explains how STED works with no success and saw this video but didn't watch because it was too long for me. After three hours of time wasting, i'm finally here and learning from the inventor. Thank you sir.
Thank you a lot! I have to prepare a exam about different microscopy methods and the chapter about STED was really unclear... just some images and a bunch of formulas! You managed to explain the basics in 5 mins!
Thanks for clearing out the confusion about the tech. I would like to use parts of the video for my presentation, if thats okay with the educational purposes.
Super educational thank you for this lovely video I just came across of it, I wonder if it would help in cytometry, We are going to do a series of experiment what are high speed camera we should definitely try to keep this in mind.
What never becomes clear in this type of explanations of STED is: as is it is not possible to produce a smaller spot of light than about 0,5 wavelength in diameter, why is it possible to make an unlit spot smaller thsn that?
It's the diffraction pattern. When creating a "donut" diffraction pattern, the thickness of the wall of the donut are still limited and set by the diffraction limit but you can change the size of the donut. So you can make the hole of the donut much smaller than the walls.
hard to think, while the green is limited to 200 nm the red light that is larger in WL can be eliminated in the small circle that is much smaller than the WL of red. i would expect that diffraction of red light make the small circle useless. confusing!
How do neurons, neo-genic, get from the rotary gyrus (places of production) to their destination? The pulsations seem para-systolic and seem likely to me that it's not only bioelectric signals being relayed .
@Rijkaard And you are a troll. @Anna Pamela de Jesus Fluorescent is not necessary, technically for RESOLFT. You can use any quantifiable property which can be turned on and off in time with nanometer precision.
The Lucas, Graton-Dane and Rife Microscopes are discussed in this article by Seidel and Winter in the Journal of the Franklin Institute: web.archive.org/web/20020811133526/www.navi.net/~rsc/seidel.htm There is also interesting work by Gaston Naessens as well on various sites worth perusing. Not to mention the incredible Multiple Radiation Microscope of Elmer Pierre Nemes.