by watching your lectures and getting inspired by u sir I have now opted Bsc biotechnology instead of medical.....now all I am dependent is on ur lectures .... an ideal guru🙏🏻🙏🏻🙏🏻
You just saved me one more time... I am attending a microscopy course where the professor is not always clear, especially for someone who doesn't a have a good enough physics background like me. After watching this, I have the basics to proceed and actually comprehensively follow the lectures. Thanks a lot, you really are a life saver for so many people around the world :)
Thank you so much for your video sir. I am messaging from Canada. Your videos provide me relief for a cell biology course im about to take. I have already failed it once and I am retaking it and just seeing and watching your videos provides me a really good idea and makes me more confident. I had this severe anxiety about succeeding in the course and with your videos I feel so much better. I subscribed and Im excited to see you hit 1million soon.
Got to your video from link mentioned in Kaunas University of Technollogy lecture slide. Proud to see Inndians recognised in Foreign universities as well. Good job bro.
Great video sir, it's really helpful. Can u make a video on how to prepare ARS NET in Agriculture Biotechnology? I know you are the only one to sort out and make a video on it ...........please sir
you said smaller the d, greater the resolution? but according to the diagram of two dots on board, as d decreases, resolution also decreases towards right side, and as d increases towards left side, resolution also increases.. please explain?
Resolution is the ability to see two things apart As we move closer to the screen (d is decreasing) the two dots placed on the board will be clearly visible as two distinct dots And vice versa if we zoom out ( d is increasing) the two dots appears one
Sir u mentioned that resolution is the ability to see two points seperately.. Then u mentioned d is inversely proportional to resolution..that means the closer the points are the better is the resolution(ability to see two points seperately) Is it that.. resolution is the ability to see two points seperately without missing it's finer details??
D means minimum distance b/w 2 points recognisable as 2 diff entities. If you can visualise 2 points clearly which are at 5 nm and other at 2.5 nm, which one would you prefer? Obviously the second one. So, d is inversely prop to resolution.
Thank you for the great video! I would like to ask what I should use as lambda in the formel for the limit resolution by epiflurescence. d=0,61.lambda/ NA What is lambda by epiflurescence: excitatationwavelenght or emissionwavelengt?
Hello sir! I have a doubt...u said, higher the angle...higher will be the resolution and on the same time, higher the refractive index... higher will be the resolution. Right? But u also said, higher the refractive index, lesser will be the angle...eg. In case of oil...refractive index is 1.51 which is higher than air and water... it's angle will be less...then, it's resolution will be high! But how's it possible? It declines ur 1st statement that higher the angle, higher will be the resolution....! Kindly clear my doubt and thanks a lot for such an amazing explanation of this topic🙏🏻
I have a doubt sir ,you say resolution is increases when we decrease 'd' ( distance between two points ) but sir if we decrease the distance between two points then points came near and nothing will be visible ,then how can we differentiate between two points Please reply me sir 🙏i am confused
Can u tell me a idea how i will get a good technical knowledge ? For example i saw your video , i took notes also but is that information is enough for getting deapth knowledge ? Or i can read more related to that topics?
What is the significance of magnification written on Objective lens (4X, 100X etc.) ? Every source says that magnification of Objective multiplied by magnification of eyepiece is total magnification. But what exactly magnification of objective lens does?
