Mushroom Spawn Preparation (Part 3/4) 

Absolutely he got the best psychedelics products 📌

Absolutely 👍 order yours on from him on instagram right away

try to find it online, or find it in a local agricultural input shop.

you're welcome. thanks for your support.

thanks for the support

Bagaimana proses pembuatan benih jamur kancing atau champignon ??

yes, generally the method is similar, including for oyster mushroom

@@ropenedu Thanks for the response and clarity 😊

thanks for the support.

allow the paddy husk to absorb some water and soften them.

theoretically, more inoculum means faster colinization. reducing the inoculum to a quarter or less is possible.

boleh. jagung kering

it can be incubated in normal tropical room temperature (25-28 degree celsius), in dark condition. Can you clarify what do you mean by filtre 0,2 micro?

@@ropenedu it’s a filter that comes on mushroom growing bags 0.2 & 0.5 filters. For gas exchange

@@user-fr7pv8qr6u I see. It's not necessary. I use cotton as filter.

@@ropenedu I’m new to all this but almost EVERYONE buys bags with filter patches. But u proved it’s not necessary thanks for sharing

@@user-fr7pv8qr6u yes, it's easier if the bag got a filter patch, but it's more expensive. you also can use a heat-resistant glass jar, make a hole on the cap, and then plug-in cotton wool... or use glass bottle, and cotton plug on the bottle's mouth.

15-20 minutes is sufficient. The rice husk is not wet, but moiste.

Brought from a seller in Kundasang. Unfortunately, I don't have the detail of the seller.

how did you sterilize the grains?

@@ropenedu Hi there🖐 1- I rinsed the grains in water, 2- I let it dry for 8 hours 3 -boiled the grains for 15 min, 4- Put the grains in glass jars 5 - I put the jars inside the pressure cooker for 50 min. 6- I let them cool one night. 7- I inoculated the grains with the petri dish culture inside the laminar flow box, then I put lids on with a hole and also put cotton inside de jar as a filter. 8 - finally i put the jars inside a big cartoon box, but 2 days later i have green mold around each piece of jelly with white mycelium that I put inside the jars Sir, do you have an email address in orden to send you some pictures? thank you very much for your attention🙏

@@maomao484 the procedure should be good. email me at rakibmrm@ums.edu.my

Yes. Can substitute autoclave with a pressure cooker. 30-40 minutes should be sufficient.

Please can I use fresh mushroom instead of cultured one?

yes, you can skip, depending on what material or seeds you use.

Thank you.

kebiasaannya nisbah 100:10:1. 100 substrat, 10 dedak padi, dan 1 kapur

Dear Sarah, it's a polypropylene (PP) bags, which can withstand high temperature with a melting point of 171 °C. Alternatively, polyethylene (HDPE) also can be used with a melting point of 130 °C.

Who can you use the double rolls bag check this

It's ok for the production of spawn. However, not recommended to use food crops as the substrate. In addition, rice will retain too much moisture, which makes them not suitable.

theoretically, spawn grown with whole rice seed (grain + husk) will give stronger inoculum as compared to rice husk alone.

yes. it's similar.

boil the risk husk to allow water to absorb and soften a bit. the cotton is to allow air exchange

setakat ni belum kami belum menjual. tuan boleh beli melalui shopee.

yes, but not practical 😂

I never try. I think it will work as well.

thanks for the advise

I think it's not necessary to add any flour.

yes. gypsum, calcium carbonate, calcium sulphate, etc. is used to increase the pH and act as anti-bulking. However, you can omit the use of these materials as well.

How much we can use gypsum and calcium sulphate for paddy husk? Ex 1kg in dry

@@j.p.n.uddipajayalath5578 1% of the weight of paddy husk (dry basis)

@@j.p.n.uddipajayalath5578 if u use 1kg of dry paddy husk, then add 10 g of gypsum.

temperature between 25-30 degree Celsius should be fine.

@@ropenedu Thank you very much, been learning how to make my own mycelium culture to try out some packaging. Your videos alongside other videos I found on youtube have been a tremendous help.

@@ForensicNA the compliment is much appreciated. Thanks for your support.

Bem-vindo

I usually use a metal tea spoon, wipe with 70% alcohol, then inoculate 2-3 spoons of the spawn. Otherwise, you also may use 1-5% based on the weight of the substrate.

yes. dark condition will enhance mycelial growth.

sorry, could you please translate it in English?

sorry, I can't understand. I hope to answer your question.

@@ropenedu thank you,my fruiting bag's is slowly disappear the mycelium?but okey when at their incubation?

@@ernestovillenajr9869 make sure the moisture content of the growing medium is good (not too wet or not too dry). Inculabe at 25-30 degrees celsius in dark condision.

@@ropenedu OK tnx

yes. similar technique

I used an autoclave. Steam sterilization at 121 degree celsius for at least 15 minutes. but the whole process from heating, sterilization, and cooling will take approximately 2 hours. Alternatively, you can pasteurize at 80 degree celsius for at least 8 hours.

Thanak you.

30-60 minutes in an autoclave (121 degree c), or 8 hours in an industrial steam sterilizer (70-80 degree c)

@@ropenedu your email id please

Wheat 90 minutes

it's a PP plastic neck for mushroom bag.

@@ropenedu thanks good day to u

thank you for your comment. unfortunately, I do not understand the language.