NAD+ in Aging: Role of Nicotinamide Riboside and Nicotinamide Mononucleotide 

*00:00:00* - Intro. *00:00:48* - Nicotinamide riboside supplementation in humans has been shown to raise NAD+ levels in white blood cells. *00:02:47* - Overview of NAD+ *00:05:12* - NAD+ plays an important role in mitigating DNA damage. *00:05:30* - Excessive DNA damage can decrease NAD+ levels by 20-30%. *00:05:42* - Lymphoblastoid cells from centenarians show increased PARP-1 activity. *00:05:55* - Between different species of mammals, higher PARP-1 activity is strongly associated with a longer lifespan. *00:06:36* - *Lower organism evidence:* A second copy of SIR2, a yeast sirtuin, gave rise to a 30% extension of lifespan. *00:06:43* - NAD+ is required for the activation of sirtuins, which epigenetically regulate a variety of stress response-related processes. *00:07:03* - Energy stress, such as from fasting, exercise, or caloric restriction, increase the NAD+:NADH ratio. *00:07:37* - NAD+ becomes depleted in the brain, heart, and skeletal muscle as we age. *00:08:13* - Decline in NAD+ levels have been associated with many broad categories of clinical disease such as neurodegenerative, cardiovascular, infectious, musculoskeletal. *00:08:48* - NAD+ supplementation has been shown to increase lifespan in models of aging in yeast, worms, and rodents. *00:09:48* - In contrast to de novo synthesis, the predominant source of NAD+ in the body is via the salvage pathway, which is subject to feedback inhibition by nicotinamide. *00:11:56* - Animal studies have shown nicotinamide riboside supplementation is able to increase insulin sensitivity, increase endurance and strength, reverse mitochondrial damage, increase mitochondrial biogenesis, and reverse muscular atrophy. *00:12:30* - Nicotinamide riboside has shown positive effects on the brain in animal, such as increased neurogenesis, decreased cognitive deterioration, decreased amyloid-beta production, and increased synaptic plasticity. *00:12:50* - Nicotinamide mononucleotide has been shown in animals to counter an obesogenic diet, improve markers of metabolic health, improve heart function, and improve cognition and memory. *00:13:10* - A 12-month dietary study of nicotinamide mononucleotide suppressed age-associated body weight gain, enhanced energy metabolism, promoted physical activity, improved insulin sensitivity and plasma lipid profile, and ameliorated eye function and other pathophysiologies in mice. *00:13:47* - NMN has been shown to increase cancer progression in a specialized senescent cell-dependent pancreatic cancer model in mice. *00:15:57* - Plausibility of dosing: comparing animal and human doses. *00:18:05* - Tissue-specific metabolism and bioavailability of NR and NMN. *00:24:32* - Oral administration of NAD produces NR and NMN in the intestine. *00:24:19* - Intravenous administration of NAD, rather than its precursors, shows interesting results: an increase of NAD content and sirtuin expression in the hypothalamic region of the brain. *00:26:15* - *Human study #1:* 250mg of NR + 50 mg pterostilbene increased blood NAD+ levels by 40%, while a double dose (500 mg NR + 100 mg pterostilbene) increased blood NAD+ levels by 90%. Additionally, there was a decrease in blood pressure, and ALT (a marker for liver health). *00:27:12* - *Human study #2:* 500mg NR twice daily for six weeks increased NAD+ levels in white blood cells by 60%. *00:29:08* - Concluding thoughts and recap.

Congratulations Rhonda you have smashed the record for total mentions of "Nicotinamide and Nicotinamide Mononucleotide" without stuttering, giggling or entering a comatose state. Kudos!

How Lucky we are to have Dr. Patrick in our lives. She is amazing. Knowledge just oozes out of her..

Concise, comprehensive, and articulate. I don't even need to speed it up to get through the information. Well done.

I'm 66 years old. I started taking 1g a day of Apigenin a few months ago. Apigenin is one of the ways to raise NAD+. It is amazing how much energy I have now. It feels like the energy that I remember having in my 30s. In addition, in conversation with my neighbors, they have commented that I seem to look younger every year, instead of older. I also take Metformin, even though I do not have diabetes. I also do a lot of intermittent, 3 day water fasts. I'm planning to start on 1g of Fisetin a day for 5 straight days every month in the next few weeks. My diet is free of meat. I do eat sardines, salmon and flounder. Lots of nuts (Walnuts, Cashews), seeds (Flax, Hemp, Chia). Dark Chocolate (80% cocoa). I do drink lots of coffee. I don't drink cow milk but almond milk instead. I don't drink alcohol. Live Long Enough to Live Foreever.

few notifications on youtube get me as excited as a video from Dr. Rhonda Patrick.

5 months later and I'm still coming back to relisten to this video. Very informative and so well put together.

I simply love your style of presenting with the latest facts. You speak fast, which I like! The data you provide and the research you do prior to presenting makes you one of the best health span advocates on RU-vid. Thank you Rhonda! ♥️

What a herculean effort and a testament to your ability to grind it out for hours on end. You’re incredible Rhonda.

Great video Rhonda, thank you for all you're doing and the quality information with such strong scientific detail.

Great presentation, thank you so much. I take NAD with R-Lipoic acid, D-Ribose and Resveratrol for a pre workout. I work out 3-4 times a week and feel as good as when i was 40, now 60.

So great to have the micro cellular pathways broken down this way. Also, the analysis of both animal and human studies. Thank you so much for the effortless way you explain potentially mind boggling processes. Helps so much in the decision making process.

This is the most comprehensive video I have seen on this topic. You are a gem. Thank you.

Excellent lecture, well put together, a good balance of pros and cons, useful diagrams and a clear summary at the end. Perfect presentation technique.

A very professional very well presented lecture . English is my 4th. language and I did not have any real problem to understand it and learn from it. Doc, I really appreciate your effort to create this video. Thank you.

This is definitely the best most informative and comprehensive piece of information so far delivered on this topic as far as I am concerned thank you for this video

This is just about the most thorough and intelligent PP presentation I've ever seen. She is one smart lady! Wow.

I really appreciate the chemical reaction explained to me along with the reaction diagrams. So rare to find anything this thorough

Dr. Rhonda this is the best explanation I've heard on this topic. So thorough and well thought out. Thank you❤

Been looking into this a bit recently. Thanks for the new upload, Rhonda! 👍

Holy crap, this was amazing. This 30 minute video took me 2 hours to get through. Had to pause and read and then pause and research. Thank you!!!

Exemplary summary. I love how dense with information your videos are. Quite a rarity on youtube! Keep up the good work and thank you for your high quality content!

NAD+ is a miracle drug and no mistake. The hype is real. It has had a profound effect on my mental and physical health at the age of 41, after many years of drug abuse, persistent old injuries, and the general exhaustion brought on by 25 years of OCD and sky-high anxiety.

Just found your channel/videos. Your presentations are "spot-on," as the modern expression has it. Loaded with information. Love your vids. I shall be watching them all. PLEASE keep up the good work !

Thank you . Please do not stop helping people the way you do.

Congratulations! Firstly for the , pleasant, patient presentation, with amazing revelations of test results of NAD, decline with age, using boosters of NAD, & Alternate pathways which work usually but with exceptions!!! Extremely A Great MASTERLY STUDY/ & presentation of all pros- cons of all eventualities . With a final recap!! Thanks for this useful educative share! Many regards!

Best unbiased recap of NAD+, NR and NMN. Thank you! Suscribed!

I like to watch informational videos like this while doing my hour of piano warm ups. Nope....can't do it on this one. Need to give this video my 100% attention. Thanks Dr. Patrick.

It appears that Dr Rhonda does a lot of preparation and compilation of subject matter for making each video. Her tone and presentation skills are excellent. She seems to be interested in sharing her in depth knowledge for the good of the people. God Bless Dr Rhonda Patrick 🙏

Best video I’ve seen on the subject. Thank you!!

Rarely do we find in the study of nutrition, detail like presented in this video, yet at the same time Rhonda is able to get to her final conclusion without leaving everyone in the weeds. Extremely well done. Combined with Dr. Nicholas Pericone and Dr. Jason Fung, we are starting to get a handle on what is necessary to make critical changes in our life for optimal health...

You remind me of my favorite med school professor- one of my favorite instructors of all time Thanks for the clear and concise video!

I could look and listen to her all day.

Thank you so much for bringing us this information Doctor. Using your great intelligence for altruistic purposes is both rare and wonderful.

So glad you covered this. Been listening to lifespan on audio book and he's one of the leading scientists on this research.

Thank you so much for the thorough long-form video, all of this information is very important.

Best presentation on the NAD/NMN potential and current status I have seen anywhere.

I've been taking 1g of NR daily for 2 years. I find that I don't get jet lag or hangovers anymore. My contribution to this fascinating science.

DOC!!!! your the best! I wish there were more docs like you.

Thank you Dr Patrick this is above and beyond anything that is out there.

The best all-in-one-video on RU-vid that explains NAD+

Great video. I am 70 and a health fanatic, and still in perfect health. I have a massive coordinated program that did not include this. Now I will be going out today and buying Niacin. I am going to start immediately. Have a wonderful day. ☺😎🐰

Because of you, I started loving myself with broccoli sprouts. ❤️ I grow them too. Sulforaphane for the win! Thank you 🥦

Thank you Dr. Patrick I appreciate the honest review as someone who was just looking into this I really appreciate the data and information you've brought forward. Please continue to be an Amazing Doctor! ❤

This is a wonderful video. We will probably be sending our patients this video when they ask about NAD+ from now on!

Dr. Rhonda, the people's champion! 😁

You are amazing!!! This material is so useful!

Stellar presentation as always, thank you Dr. Patrick!

Wow amazing job and the best, most concise source of information I've ever seen for NAD oh, great job!

It is a fascinating topic and I love how scientific Dr. Patrick approaches it!

Damn. I need an IV of this channel straight into my brain. Oh, and that other stuff you were talking about, as well.

What a great video doc. I saw you in person once but did not want to bother (Fashion Valley). I would consider you to be one of my hero’s in the health field… I’ve been greatly inspired by you and Dr.Sinclair to understand health from a scientific basis in hopes that I can pass this knowledge onto my mother who has MS and the rest of my loved ones. Thank you for what you do.

Excellent and brilliant work. Thank you very much indeed. The literature reviewers are amazingly detailed and strikes the key points of the findings from the researches.

What a masterpiece! Thank you!

I'd like to see you do a video on the role of Niacin (Nicotinic acid) on supplying the molecules for the manufacture of NAD+

Exciting developments. Great to know scientists are working so hard on our behalf in this crazy world. In the meantime I'll continue my exercise, sauna, and clean diet. Thanks!

Superb. So much information explained so efficiently in 30mins. I feel very well informed now, thank you!

Dr. talks to us like we could really get it....I do!!!

Thank you for such an educational clip . Please keep it up

Thank you, Dr. Rhonda Patrick! Great information!

Good work! You certainly did the research and can explain it with great accuracy. Thank you.

Excellent lecture. Thank you for this presentation Dr. Cheers

The year is 2063, Dr Rhonda Patric still looks 23 and now posseses levitation has awakened her 3rd eye.

THANK YOU SO MUCH!!!! I AM LOOKING FORWARD TO VIEWING OTHER VIDEOS FROM YOU!!

Amazing content as always Rhonda.

Amazing video. Thank you for the brilliantly explained content.🇿🇦😎🙏

I’ve been taking 750mg of NMN + 500mg resveratrol for 6 months. I’m 61 and in good, but far from great shape. My muscle stamina and overall energy are way higher than when I began. I also have noticeable reduction in inflammation in my leg that was damaged in an accident and where it had been chronic for many years. The reduction in inflammation alone keeps me on it, but feeling a lot better is great too.

Thank you for being honest and thorough. You are the best

Thank you Rhonda I will watch this again you explain NAD+, NR and NMN excellent easy to follow. I will come back to this video on referencing one of the best out there. God Bless you.

I've been taking a low doseage of NMN for a couple of weeks now. I started with 100mg in the morning and 50mg at night on the first day, then 50mg the next morning. I hadn't been expecting any results for at least a week or so. About 2 hours after taking that second morning dose I went out shopping and was astonished to find my legs wanting to walk faster and faster, as if under their own volition. I've not walked like that in almost 7 years. I've had a number of strokes and suffer numerous symptoms, manly to do with inhibitted activity levels. My mind is foggy most of the time and the NMN has not done anything for that, but I certainly feel the difference in energy levels, it's really amazing. I also found that after a few days I had to stop the NMN for a couple of days as it seemed to be making me feel more groggy, so I now take it 2 days on, 2 days off. The brand I'm using is an American Brand called RevGenetics, and it's called Advanced NMN, in capsule form. I know it's also available in powder form too. It's quite expensive even in capsule form and would cost the earth to take it regularly at the doseages recommended in this video, but I can assure anyone that the effects at the doseage I am on are truly amazing.

I'm exactly 180lb, thank you for this vid!

I think you can't make more concise and comprehensible than this

So helpful + informative for learning about aging. Thank you for this video!

** Due to a serious case of mold poisoning I have been on NAD+ IV's for 6 months... they literally saved my life.. I have never had oral NMN or NR. However I would highly recommend anyone with a health issue try NAD+ IV's. There are numerous accounts of people turning their life around due to NAD IV's not precursors. Best wishes to each of you!

You’re so smart it’s scary!!!!!

Great concise presentation as always. Thank you.

Awesome, best NAD video so far, you saved days of research in such information dense video, awesome work

I weigh exactly 180 pounds... this is great.

Thank you for the video. I have been taking Tru Nigen (NR) for about two months at 350 mgs. What I have noticed is a slight increase in energy, and focus. I feel like I have also lost some body fat. However, I stay in Ketosis and use intermittent fasting 4 days a week. However, I plan to stop taking it once my bottle runs out because of the information presented in this video. I don't see the benefits outweighing the potential downsides. Thanks again.

its bonkers to conceive that this is free. ty Dr Patrick

Thanx Rhonda, for an excellent presentation ! I've heard about this mainly from David Sinclair - I am following closely, and I look forward to more input from you !

I personally get NAD IVs@24:30 and use them with my patients and notice an increase in energy and improved cognition.

Every time she says "enzyme" you have to pop an NR capsule

Love the videos! Still want to see more Dr. Ames!

Very helpful - comprehensive - much appreciated!

Please Rhonda, the Floxing community needs you to make a video about Fluoroquinolone Toxicity!

She’s awesome

Thank you so much for giving this teaching on this topic. Valuable information!🥰

That was a very thorough explanation Dr. Patrick. Thank you.

Great video Rhonda! I was wondering if it makes sense to take a NR/NMN supplement while also ingesting carbs/protein/fat. As you also explain in the video, the NAD/NADH ratio in the cell increases when fasting/CR/exercising. Then such a NAD boosting supplement would need to be taken in a fasted state wouldn't it? Or is the pathway of either NR/NMN supplement somehow separated from the metabolic pathway when a cell is in a fasted state?

Looks like more studies on humans are needed since the animal studies seem so promising. I've been taking nicotinamide riboside (250mg) and pterostbene (50mg) for over 5 years now. It's the only supplement I take. I'm 66 years of age, 5'10" tall and weigh ~155lbs. I feel in perfect health despite a few creaks and hiccups in my giddy-up (heh). I'd also like to think my diet, while moderate, is well-rounded in a healthy sense. Lots of fruits, vegetables and a modicum of meat. I'm not much into junk foods and the like. I sleep well at night, averaging ~8hrs, and function well all day long. My energy levels remain constant pretty much all day, though I'm not going to claim I have the same level of endurance I had at 18-20 years of age. I bike and walk for exercise and pleasure. I suffer none of the maladies I see beginning to manifest in friends and colleagues in my age cohort. And I still have my marbles. Or.....at least I think I do. Friends may disagree. ;-) Now is this due to the supplement? Possibly. Physically I feel like my body is functioning at a nice "steady state" level though the vicissitudes of time do appear when I look in the mirror. There's nothing to be done, overall, about the ever constant, albeit slow, aging that it shows. Gray hair, wrinkles and the like. That's just life. That said I feel like I'm motoring along nicely. But in the interest of full disclosure I'm unclear if this is entirely due to the supplement I'm taking. A portion of this might be due to it; but I've a 90 year old father who still runs +20 miles a week, every week. As he has done since I was a toddler. And my mother is still alive and kicking, though a bit more frail. Both still have their minds, too. So the supplement might be noteworthy, but I suspect so, too, is my genetic coding. So that needs to be considered when evaluating the merits of my health. In any case I see nothing wrong with supplementing the diet with it. I take no vitamins and eat a moderate, rounded diet. It would be nice to see the results of more human-oriented studies of nicotinamide and the like. I think there's merit to it keeping one healthy for as long as one lives. And regardless of how long you live staying healthy is the whole point, isn't it? John~ American Net'Zen

Congratulations 🎊 - It's pretty rare to be able to see such good quality presentation for so complex topic and compressed in so condensed time

Loved your interview with Dr. Daivid Sinclair! The meeting of two great doctors! Hope you have another with him! Maybe get a pre-copy of his new book to come out soon for an interview?!

I just hope she appreciates me keeping this comment classy. -Thank You, Rogan again soon?

For very different reasons, NAD+ has also attracted a wave of attention from cancer researchers. Recent studies suggest that cancer cells of many types depend on NAD+ to sustain their rapid growth and that cutting off the NAD+ supply could be an effective strategy for killing certain cancers. The data from these studies paint a more complicated picture of NAD+ and raise new questions about the diverse ways taking an NAD+-boosting supplement might influence health. “It might still slow down the aging part, but it might fuel the cancer part,” says Versha Banerji, a clinician-scientist at the University of Manitoba. “We just need to figure out more about the biology of both of those processes, to figure out how we can make people age well and also not get cancer.” This is just a fellow up to my previous post. Interesting that most sites are ignoring the possibilities of its effects on cancer.

Thank you! This is wonderfully insightful.

Amazing skills to flood my brain with huge amounts of information. I know how its gonna feel when AI's are smarter than us all its the same feeling I get when I listen to Dr.Rhonda

Very good and objective presentation from Dr. Patrick about NMN supplement, unlike Dr. Sinclair, who seems just want to promote and present NMN information in a selective way.

Wow, thank you for that full explanation. I have cut sugars, am into intermittent fasting, with 4 prolong 5day fast a year, and now looking into Mr Sinclair's research. I don't look anywhere my age, I'm told. Exercise and eating right is crucial. I am a nut at getting my DNA and any acetylation or methylation repairs done. Doing the autophagy route for this right now. I was thinking of these surpluses, but I think, I'll stick with my diet and exercise routine. Thank again, great explanation, backed with studies. I'm loving you already!

Dr. Rhonda - you are such an amazing speaker and i have learned so much from you. I just want to respectfully remind you that the word "associated" does not have the "sh" sound in it. So many people make that same mistake so probably most don't notice it.