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Northern Blotting - Biology Tutorial 

Joao's Lab
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22 сен 2024

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Комментарии : 75   
@jonqn07
@jonqn07 7 лет назад
thank you VERY MUCH for your hard work!
@steevensonbeaubrun6539
@steevensonbeaubrun6539 9 лет назад
With this piece of video, you just saved my Genetic Engineering exam. Good job!
@lenus8D
@lenus8D 8 лет назад
thanks very much....finally understanding it!!!!! and it isn´t that complicated as it looked during the class :D
@kimtran9504
@kimtran9504 10 лет назад
Thanks so much! Super helpful for my test! :D
@MariaCojocaru-e8k
@MariaCojocaru-e8k 8 месяцев назад
Fantastic video. Very detailed, has all the key information and it's broken down simply :) Thank you.
@JoaosLab
@JoaosLab 8 месяцев назад
Glad it was helpful! 🙌
@gemininga92
@gemininga92 8 лет назад
amzing, very usefull for me and all people who speak english as the second language.
@andrealombarteserrat7621
@andrealombarteserrat7621 10 лет назад
Great video, thank you so much for that, usually scientists have to learn all the tecnics that we must use for yourself, and this video helps a lot!
@promisendlovu8550
@promisendlovu8550 8 лет назад
lol....."dis is not a harmburger trust me" really enjoyed dis video.....thank you very much Sir !!!!!
@unicornsforreal
@unicornsforreal 10 лет назад
Very easy to understand.
@JoaosLab
@JoaosLab 11 лет назад
Thank you! :) I'm glad you enjoyed it!
@jamesyen98
@jamesyen98 5 лет назад
Thank you for uploading this video. It is very helpful to understand the concept.
@ignatiuswayiti2862
@ignatiuswayiti2862 8 лет назад
U the man! i get every word through the video... keep doin what you doin..
@nicstandby
@nicstandby 11 лет назад
Please, make a video about western blotting! Great video and thank you!
@kevinraymondoluoch4319
@kevinraymondoluoch4319 9 лет назад
Great stuff!
@CACHATITAZITO
@CACHATITAZITO 10 лет назад
Great video! very clear... I couldn't find the southern blot video. If you have the link, I'll be glad
@farmaciaufg878
@farmaciaufg878 10 лет назад
AMAIZING
@gb546733
@gb546733 9 лет назад
wow! great tutorial
@JoaosLab
@JoaosLab 11 лет назад
I believe the filter helps with the labeling treatment.
@Sharkbait173
@Sharkbait173 8 лет назад
Great vid. Thanks!
@nevergone07
@nevergone07 9 лет назад
thank you so much. it helpful for my seminar^^
@Nilay_Niloya
@Nilay_Niloya 9 лет назад
Clear, practical and well detailed! Thanks a lot!
@apoorvasastry7073
@apoorvasastry7073 10 лет назад
Loved it! Extremely well explained.
@molekulqrnabiologiq9912
@molekulqrnabiologiq9912 7 лет назад
this video was very helpful,thanks :)
@ahmadfares5243
@ahmadfares5243 2 года назад
Thanks, it's very helpful and clear
@JoaosLab
@JoaosLab 2 года назад
Glad it was helpful! 😊
@AppyGal19
@AppyGal19 11 лет назад
this video helped me soooo much! you used the correct terminology I was looking for. I appreciated the help!:)
@JoaosLab
@JoaosLab 11 лет назад
Glad to read that! We love their images too! :)
@akashjustforyou
@akashjustforyou 11 лет назад
great piece of work..really appreciate....simple and up to mark...
@martinbakht2214
@martinbakht2214 10 лет назад
Thank you!
@Blueberry31679
@Blueberry31679 9 лет назад
Very helpful and clear! Thank you!
@nhunghoang2030
@nhunghoang2030 9 лет назад
Very useful. Thank you very much for the guide
@Morinamora
@Morinamora 11 лет назад
you saved me, thank you!
@robhitsemup
@robhitsemup 10 лет назад
Awesome!!!!!!
@domenicoraddi6601
@domenicoraddi6601 8 лет назад
This was really good. Keep it up!
@Fleshjesus
@Fleshjesus 10 лет назад
Your video on southern blotting is offline. Can you please reupload it?
@sahay1madhulika
@sahay1madhulika 4 года назад
Good explanation.
@JoaosLab
@JoaosLab 4 года назад
Glad it was helpful!
@Flynnbojangels
@Flynnbojangels 5 лет назад
What are the limits of detection of this experiment? Eg. sensitivity? What if you're trying to detect small siRNA or miRNA?
@ThuyPham-gf3ez
@ThuyPham-gf3ez 6 лет назад
Can we profile multiple RNA with this rechnique or it is only once at a time? Thanks
@JoaosLab
@JoaosLab 11 лет назад
You are welcome!! :)
@Nantidan
@Nantidan 10 лет назад
is it possible to use SDS-PAGE instead of formaldehyd when openining the hairpin-structure of the RNA? (time 9:10)
@bismaazam5303
@bismaazam5303 7 лет назад
in pcr we used primers that bind to the specefic complimentary dna sequences. but how can a cdna bind to rna . i dont understand
@JoaosLab
@JoaosLab 11 лет назад
You're welcome!
@brunaantonini
@brunaantonini 10 лет назад
Which computer program is used to explain this technique?
@oyeman1000
@oyeman1000 11 лет назад
Loved it . Great video thanks
@Nina_SL
@Nina_SL 10 лет назад
You just saved my life!!
@imagination7710
@imagination7710 8 лет назад
Does formaldehyde just act as an acceptor of hydrogen bonds between base pairs?
@magorzataprus163
@magorzataprus163 10 лет назад
Really helpful I love it !
@BT-no3eh
@BT-no3eh 8 лет назад
is there a video for western blotting that is this good?
@JoaosLab
@JoaosLab 11 лет назад
Thinking about covering that topic soon! :)
@alfonsoortizcruz6116
@alfonsoortizcruz6116 11 лет назад
Great video! Thank you for the tutorial. God bless.
@nalindy254
@nalindy254 6 лет назад
Excuses me !! This video explain about sample preparation of northern blot right ??
@k1morisato889
@k1morisato889 3 года назад
nice job
@pancreasdragonheart9765
@pancreasdragonheart9765 11 лет назад
Does these filters/membranes has those labeled probes? Or what's the point with the filter?
@michaeltolkachov4749
@michaeltolkachov4749 7 лет назад
Cool, though usually amino benzyloxymethyl filter paper is used instead of nitrocellulose due to its higher affinity to RNA :)
@maibar-chen6528
@maibar-chen6528 9 лет назад
Hi! great videos! Where can I find the southern blot tutorial ? Thanks!
@42marthacu
@42marthacu 9 лет назад
how do you extract rna and not dna?
@Pettyelettrodancer
@Pettyelettrodancer 11 лет назад
very clear!!! thanks!
@a.l.a.acunahamann2031
@a.l.a.acunahamann2031 7 лет назад
Genio!! GRACIAS!!!!
@gabrielaferraz7963
@gabrielaferraz7963 7 лет назад
Muito bom o texto/desenhos e apresentação, fácil compreensão do ingles. Parabens!
@afnankhalis1875
@afnankhalis1875 10 лет назад
a lot of your past videos, including the one about Southern blotting is no longer available, why?
@uhRoid
@uhRoid 6 лет назад
where is your southern blotting video?
@questthefuture
@questthefuture 10 лет назад
very great presentation, I appreciate the sense of humor :) , correct me if I am wrong, should the cDNA be a complementary sequence?
@laxballdmw
@laxballdmw 11 лет назад
Nice vid...well polished too.
@vannguyen-gs3so
@vannguyen-gs3so 5 лет назад
wait arent u from kenhub?
@unicornsforreal
@unicornsforreal 10 лет назад
How would this work with siRNA?
@unicornsforreal
@unicornsforreal 10 лет назад
This question is for anyone who is an expert with this stuff.
@yazminbrito1405
@yazminbrito1405 10 лет назад
why is the reason of the salt solution? :)
@michaeltolkachov4749
@michaeltolkachov4749 7 лет назад
The salt (also buffer) solution allows an ionic transfer to occur. By capillary movement provided by the sponge with a weight pressure negatively charged RNAs transfer along with positively charged salt ions. RNA cannot pass through the amino benzyloxymethyl membrane and remains there, while other ions are absorbed by the filter paper. I hope it'll help :)
@yazminbrito1405
@yazminbrito1405 7 лет назад
Thank you!
@aches6652
@aches6652 9 лет назад
why we use paper towels ?
@kirkury
@kirkury 2 года назад
Is Roger from American Dad narrating this?
@JoaosLab
@JoaosLab 2 года назад
Yes. 👽
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