I think there is a mistake on the last step, the one which involves the 2,4-dienoyl-CoA reductase: the reaction requires a NADPH (+ H+) to reduce two of the four carbons involved in the double bonds, instead of NADP+, otherwise that would mean a further oxidation of already oxydated carbons.
Yeah, you're right. Because you're adding two Hydrogens, one at carbon 2 & the other at carbon 5, and the electrons must perform some sort of radical intermediate in order to form a double bond between carbons 3 & 4. Simple error.
Thank you very much for your lectures. Sometimes books take for granted facts and explain mechanisms and processes without giving any reasons for what is happening. For example, in this case, I could not understand why a reduction reaction was needed. You simply explained that it was because the conjugated intermediate was not a substrate of the enzyme hydrolase and so a different reaction was needed. I don't like learning without thinking, only memorizing stuff putting no logic into it. That's what I like about you. You always explain WHY something is happening. You're an inspiration. Thank you very much. I'll pass my exam thanks to you.
Welcome back!I was wondering when the next video would be uploaded,I am a student studying at a difficult country, and I get a lot of help from you, Thanks! :D
With this knowledge you're the most suited scientist to develope supplements and medications to correct the missing links in our metabolism . I loved organic chemistry in school.
or how about to eat a diet that would be best suited for health, to eliminate the need for medications? Who wants to be on medications for life if health was an option all along?
thank you for posting this video, thank you for coming back, a bit of correction, when enzyme 2,4-dienoyl-CoA reductase works, it requires NADPH + H+, forming NADP+. not the other way around, please check. thank u
+AK LECTURES (Andrey K) i've checked d video, and you really add the annotation, thank u.. anw i'm still curious how a math grads can end up in med school? =D
Thanks so much. Your videos have been beneficial to me as a Nigerian postgraduate student. In the reaction involving 2,4-dienoylCoA intermediate, NADPH should be used up to generate the oxidized form. Thanks.
I don't see the point on the even or odd numbers of double bonds. The illustration that @AK Lecture showed for the even C18:2n6 can be also applied to C18:3n3. Can someone helps to clarify this point? Thanks
Great video it explains a lot but I just don't get it how double bond from last step when reductase is working how cis double bond between fourth and fifth carbon jump to the trans double bond between third and fourth carbon because reductase just give hidrogen atoms to the molecule it is not removing two carbon atoms
Great stuff, thanks a lot. I was just wondering if all dietary PUFAs are equally readily oxidized for energy production, or if the number of double bonds (or other competing metabolic functions, e.g. in case of AA, EPA, DHA) affect the utilization. In other words; if one didn't eat enough calories, would his omega-3-supplements be as readily catabolized to energy as any other fatty acids?
The palmitoleic acid goes through 3 rounds of beta oxidation until the cis acyl coA is formed, and then the enoyl coA isomerase, then continues beta oxidation etc.... but Is it 3 rounds for this particular example or do all mono unsaturated fatty acids go through 3 rounds?
If 1 NADPH => 3 ATP and 1 FADH2 => 2 ATP So Stearic acid => 146 ATP Linoleic - 1 FADH2 => 144 ATP Linolenic acid - (1 FADH2 and 1 NADPH) => 141 ATP Right? Can everyone tell me please? Thanks (Linolenic acid -2 FADH2 because it presents 2 double bond, and +1 FADH2 -1 NADPH in dehydrogenase and reductase step)
1. What occurs to poly and monounsaturated fats that go unused? Are they converted into triglycerides? 2. Say someone is on a ketogenic diet, consuming large amounts of fat. What is the signal that induces storage of fat vs oxidation? Such as how maxed out glycogen storage results in triglyceride formation, in terms of carbohydrate consumption.
