pGLO Bacterial Transformation Lab

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Here I discuss a popular bacterial transformation lab using the pGLO plasmid. The video includes a walk through of the protocol as well as background information on plasmids, operons and experimental biology. If you are confused on any aspect of the lab or want to compare results this is the video for you.

Опубликовано:

4 июл 2024

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Комментарии : 47

@ginareyes6762 5 лет назад

This is the best explanation for this lab! Makes so much sense and helps visually! Thank you so much Doc Ron Bio.

@vspianist 4 месяца назад

I have a strong feeling that this is going to be the only thing asked on my next biology test, and I knew that I had to understand this or I'm doomed. This video helped so much!

@ecsl9796 Месяц назад

i had an emergency the week we discussed this in lab so i had no idea about this. I have my final in 4 days and this really helped me understand the whole lab :,) thank you!

@FPtz Год назад

Wish I had found this video before my lab. After many other videos everything was still unclear. After this video, i fully understand and can answer all of the post Lab questions!

@raquelvargas487 4 года назад

Thanks so much! I have an exam based on this lab and this really helped tie it all together.

@abigailb8102 5 лет назад

Thank you so much for this video!

@surat8591 5 лет назад

Thank you for explaining everything in detail and actually articulating each word. I had a similar lab last week, but I didn't really understand the promoter and regulator part until now. Thank you! I wish you were my professor 😍😍

@DocRonBio 5 лет назад

Glad to help!

@tennyvasghanian3015 4 года назад

Such a clear explanation! Thank you!!

@DocRonBio 4 года назад

Tenny Vasghanian I appreciate the feedback. Glad I could help!

@pkre707 7 месяцев назад

Did this lab a few weeks ago. So much fun!

@peterl5337 3 года назад

Amazing, you made it quite easy.

@PA30000 3 года назад

Thanks, this was nice and simple, panic averted.

@__zelda__ 4 года назад

I have an exam tomorrow and this was so helpful! Thank you!!

@DocRonBio 4 года назад

Zelda Fogle Glad to help! Good luck on your exam!

@thesavvybrand 5 лет назад

Thank You!

@hillary7261 5 лет назад

Excellent! Thank you so much!

@DocRonBio 5 лет назад

H M Glad it was helpful!

@andyzzh8622 2 года назад

Love this!Thank you soooo much!!

@DocRonBio 2 года назад

Happy to help!

@royarad1554 5 лет назад

Great video!

@DMorningStarWithAyandaSithole Год назад

Thee best, thank you so much!!!❤

@user-xj3si7ew4g 3 месяца назад

Great explanation!

@XTheSpartanX7 4 года назад

wow this was great

@montruc8082 4 года назад

Thank you ❤️

@DocRonBio 4 года назад

Mon Truc You are welcome!

@graycenamos1166 2 года назад

super helpful!

@sandisonkosi9835 5 лет назад

Doc Ron I thank you Sir

@DocRonBio 5 лет назад

Glad to help! Good luck on the exam!

@evelynli2053 Год назад

this video was too good

@alannaburns4725 2 месяца назад

Amazing video!

@DocRonBio 2 месяца назад

Thank you! I appreciate it.

@cindyseymour5863 3 года назад

Please consider correcting the captions! Extremely important for students with certain ADA accommodations. For example "pGlo" is automatically captioned as "pee glow" :-).

@Avm2 Год назад

What is the independent variable?

@champ1177 5 лет назад

How would I use my knowledge of natural selection to explain why the LB+ plate didn't glow.

@DocRonBio 5 лет назад

LB+ never received pGLO, thus they do not have the genes for that phenotype.

@juelb4658 5 лет назад

do we get the same transformation efficiency for the last two plates? so for LB+amp and LB+amp+ara

@DocRonBio 5 лет назад

Hello. Yes you should get roughly equal rates of transformation on these two plates (roughly 75 colonies). The plasmid being transformed is the same in both. The arabinose inducer is present on only one plate (LB+apm+ara), but his only impacts expression of GFP, not the transformation (which happens in a previous step). Transformation may, however, differ in each group and from plate to plate based on how well students follow the protocol (but not because of the molecular biology).

@juelb4658 5 лет назад

@@DocRonBio That makes sense, thank you!

@iqrarafiq7065 4 года назад

how do we know that LB is the positive control instead of being the negative control

@DocRonBio 4 года назад

Hello Iqra. In general, controls serve as a comparison or reference for your experiment. When the control serves to show the absence or loss of something we generally refer to that as a negative control. For example, when performing a chemical reaction of some kind, if you leave out a key reactant, showing that the reaction stops. In the case of pGlo, showing that without the plasmid the bacteria die because they do not have the gene for the antibiotic resistance trait. In the case of the positive control, we do this to show when some thing is working or present. For example, showing a previously confirmed functional reaction side by side with a new, experimental one. In the case of pGlo, we show that the bacteria are alive and able to be seen on the plate, even after heat shock. Otherwise, someone might argue that there never were bacteria to begin with in the experiment! Or if they are not present in the positive control you can show that there is something wrong with the bacteria to start. I hope this helps to clarify things!

@iqrarafiq7065 4 года назад

Doc Ron Bio thank you so much !