Andrey, you have a real gift for teaching. I was glad to donate to your site this evening, as AK Lectures is one of the absolute best sources for instructional science content on the web. Thanks for making us all MUCH more knowledgeable!
+Daniel Holley Hey Daniel, thanks for your donation, I greatly appreciate that! Thanks for all the kind words, happy that my content is helping! Have a great one!
I've been using your lectures throughout my undergrad, and now I'm in 1st year of 'Biochemistry & Molecular Genetic' PhD program. Yet, I still find your lectures very helpful. Thank you so much! 🙏
ak u are the best teacher i have ever learned from. Now i have a hope that i would get very good marks in my boards this year. If that happnes i wauld never forget u in my whole life, i hope you keep on making us so knowledgeable, thank u
thank you very much for these lectures. every day i watch these videos. i am a biochemistry student. and these videos help me a lot. again 1000 thanks.
Honestly, if it weren't for you I don't know how I would pass my classes. Thank you for your organized lectures. You are literally the best on youtube!
@AK LECTURES can you please tell us, Just roughly,What is the quantity of dNTPS and Primers required per cycle? Because the no of strand keeps getting amplified after every cycle,so that means more dNTP's,polymerase and Primers required. I will be really grateful if you reply.
Picked this video because I just KNOW Andrey will deliver and I WILL understand by the end of the video. Huge props to this channel for being the best at explaining hard biology concepts!
YOU ARE AMAZING. You are saving my life in biochemistry, physics, and molecular biology. No one has ever explained anything better than you. Thank you!
Don't know what else to say, i found PCR complicated before---you could not have made it any easier to comprehend----What temperatures do regular Polymerase operate about 37-40 degrees?---why do we need the heat resistant if step 1 and 2 has been achieved is it to minimize annealing issues??
Thank you so much!! You are the only one on RU-vid to do that kind of awesome videos of avanced biology!! I speak french and i understand everything you said, thanks a lot!!
Honestly, it's because of you I understand my genetics class. After reviewing my slides and listening to your videos it puts everything into perspective and understanding
You truly are gifted sir. I’m currently a PGY 1 surgery candidate and I’m having to do an entry basic medical science exam. Straight to the point. Short & clear. Big 👍🏾 from 🇵🇬.
But I dont understand how the primers are created... how you create those specific chains? Its hard for me to visualize how the nucleotides are created to be all same, or how you create a radioactive nucleotide or how you dye them
GREAT VIDEO AND INSTRUCTIONAL PRESENTATION. This written and oral demonstration is good up to a point but would it be possible for you to do an ANIMATION so we can VISUALLY see exactly how this PCR process unfolds. Also would you be able to do a video explaining how this PCR technology is used for practical purposes and circumstances for instance,to identify the guilty suspect in a CRIME scene and secondly, how it would used to DETECT MINIMAL RESIDUAL DISEASE in LEUKEMIA for example. Thanks.
Andrey K Thank you very much for your wonderful and very useful way of explaining the information. I always use your style of explaining information to my students
is it correct to state that in order for the primers to bind on the correct pieces of dna (the flanking sequences) they first have to determine the base sequence of this dna strand so they can 'make" the correct primers? cause i dont see any other way the primers could know where exactly to bind on the dna strand
+Arne Scheire you are correct that RNA primers have to be a complementary of the flanking DNA bases for them to bind properly. Remember that there are different types of DNA polymerases and and a specific class (DnaG for bacteria, Primase/DNA for eukaryotes, and primase for for archaea) is responsible for the synthesis of primers.
One question , 72 degrees is the ideal temperature for the DNA polymerase so initially while increasing it to 94 degrees at 74 degrees do we observe change? like in the first step because the temperature will definitely be at 74 for a few seconds at least . Will the polymerase get acivated maybe?
thanks you have just explaned what it took my lecturer 5 hours to explain . love that you take time to reapeat concepts and I am thankful for the drownings helps a lot with remembering. and of course you are a grate teacher
thank you sooo much for all your videos please l want to understsnd relative and absolute quantitation,efficiency,melting curve analysis, multiplex please explan 😢
after the reverse transcription, we get a single-strand cDNA. I'm quite confused about how to get double-strand cDNA by PCR, cause in the experiment we use BD smart RACE cDNA amplification kit to do this, but I don't konw the function of NUP Looking forward to you reply! Thank you so much
