I have been watching a lectures for a few weeks now, i realize that he is actually a but of a genius. Maybe we don't recognize all of them in society and some of them could be teachers like him, but he seems to be a person with multipotentiality. Intelligence, clarity, diverse subjects, hats off to this great man!
No, DNA is not single strand. What we do first is to create the ssDNA complementary to the ssRNA. We now have to destroy the ssRNA to build the complementary in DNA. That is why we use high pH to eliminate RNA and only obtain ssDNA. Then with DNA polymerase the second strand of DNA is created. Now we have dsDNA. Nothing of this means that DNA is simgle stranded, only when we need it to be in the process using heat.
Maybe already have long time this video but I have a question, and I hope someone can answer me. There's no technology to create the protein using our eucaryotic mRNA and to avoid the other steps to get a protein (eucaryotic)? I mean, if we can extract the mature transcript (modified mRNA -3' poli A tail, 5' cap and spliced exons) from a eucaryotic cell, why we need to form, make or any word you want to use, cDNA and then use procaryotic cell?, unless there's no technology to use directly the mature mRNA. Thanks
because retroviruses can insert specific DNA fragments into the host original DNA, this means everytime the host cell reproduces it will have this new modified DNA which can be translated in the new RNA , if you directly insert mRNA it will only be used once because mrna degrades over time
Most understandable lectures that I have found in my life, so clear explanation, I go through most of the times with the lectures what i need in my exams,, best wishes for him always
you are a legend!!!!!!!! I got an exam tomorrow and wasn't too sure of the reason cDNA was created and how it was created, keep up the great work!!!! You will achieve great heights my friend!!!
Your video has a mistake. You said that for the eukaryotic mRNA, the exons must be spliced out but it's the introns that are spliced out during the post-transcriptional modification to produce the processed mRNA. time 2:30
That's not a hard-fast rule. mRNA can undergo "Alternative Splicing" where exons are either spliced out or left in the mRNA. It is based on the splice site that the spliceosome decides to function at.
You have some excellent lectures! Could you do one on YAC's and BAC's. If you have already I haven't been able to locate it. Thanks for you teaching methods!
How can we know that the poly T tail won't bind to the poly A tail of another mRNA molecule rather than the mRNA molecule of interest since all the mRNA molecules have a poly A tail
You don't. Poly T-tails will be used for non-biased cDNA library generation. If you want to make gene specific cDNA only, you will need a 3' reverse primer that is sequence specific.
Excellent teaching skills!!! You make molecular biology to look very simple and understandable. Great job which arises from a good knowledge. Thank you.
Why do we need to synthesise poly c tail dna primer?? We can use poly a tail dna primer it is complementary to the cDNA.. And we don't need to add terminal transferase
+QuentinWolffMusic no it won't be a problem because the translation of the mRNA formed from this cDNA would have a start and stop codon flanking the gene of interest, so any no. of dNTPs before the start codon won't alter the final protein. I hope ths,helped
Why couldn't we just directly isolate a DNA sequence that will produce the required mRNA? Instead of going from mRNA to cDNA to go back to mRNA that is needed?
Hi. I want to sintetize de Oligo dt(12-18) and Random Primers (d6)n and save some money. How do I have to configure de requirement (order) for the primer syntesis lab? I know that the 5' of the oligo dt must be modified wiht a PO3, but that's all I know lol... Please, help me
Awesome video! Thank you for making it. This helped me tremendously to prepare for my national exam. I'm not the type that can just read about a process and grasp it; I have to see it or perform it to truly understand it. Thanks again!
I am finding myself SMILING AT DNA TECHNOLOGY because you are explaining it so beautifully, reiterating things so we mortals can keep up and tying concepts together from film to film. Awesome. You are the go-to channel for my UK A level science teaching prep. Just made a bit of donation.
Damn, it was all that easy. That guy from 8 yrs ago, just destroyed my instructors 1 term period teaching skills in 12 minutes. Respect man thanks a lot.
since the revierse transcirbatse need a primier to start doing its jop whats if we inserted a normal dna polymerase instead of the RT wouldnt it do excatly the same process of making complementry dna to the mrna ?
I don't think DNA polymerase will do its job on a single-stranded mRNA, as it cannot use the mRNA strand as some sort of 'template strand' (mRNA strands contain uracil instead of thymine). So that is why reverse transcriptase is used