Prokaryotic Transcription INITIATION - How do sigma factor & RNA polymerase initiate transcription?

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The process of the transcription Initiation in prokaryotes. I explain the structural features of sigma factor 70, RNA polymerase (core- and Holo-enzyme) and the promoter DNA configuration and transitions. The main stages of the initiation process discussed are Closed Complex formation, Open Complex formation (with Transcription Bubble), Ternary Complex formation, and DNA scrunching.
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00:00 Introduction
01:20 Sigma factor structure
02:15 RNA polymerase structure
03:28 RNA polymerase holoenzyme
05:08 Closed Complex
07:39 Open Complex
09:40 Ternary Complex
10:30 DNA scrunching
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3 июл 2024

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Комментарии : 52

@okpolihenry983 2 года назад

YOU ARE VERY GOOD AND DETAILED . THIS IS FOR GRADUATE STUDENTS NOT THE USUALL SIMPLISTIC ONES WE SEE ON RU-vid

@avonleastudy6535 9 месяцев назад

Exactly. it is so detailed and easy to comprehend. Thankyou the crux.

@adrijabanerjee-ek6hw Год назад

This is for masters and higher studies. Exactly what we need. Amazing Explaination

@user-ig6or1sm5q 3 года назад

your videos are so clear and interesting! thank you so much!!!

@sumaib1 2 года назад

well prepared and well delivered. I have to appreciate your drawing skills. Thank you.

@YourLocalLeo Год назад

quick, clear and concise. great chapter organization, diagrams, everything is great.

@sudhavani4306 2 года назад

Best vlog on transcription initiation

@Audriavbruna Год назад

SO HELPFUL MY GOODNESS!! THANK YOU!

@gabrielatrevino9785 3 года назад

love these videos! seriously saving my grade in genetics!

@claudiamorse Год назад

this was absolutely amazing thank you so much

@mdshanewazhossan1894 3 года назад

Excellent video brother.

@user-fs1wr5ho9m 2 года назад

You literally save my biochemistry! Thank You!!!

@santoshhuded9688 4 года назад

Nice explanation 👌

@physicaleducationrewari3299 2 месяца назад

Arey Bhai shab gajab h gajab

@okpolihenry983 2 года назад

OH MY GOD!!! , THIS IS IT. YOU GOT MY SUBSCRIPTION

@tanvirahmed617 Год назад

sir you r extremely helpful and so detailed . watching you from bangladesh .

@Sunshine-hc3lt Год назад

Thank you so much

@Sweety__Tiwari Год назад

Very helpful, thanks😇

@ammarahmehak9986 4 года назад

very nice video, keep it up

@ayeletchenchicheportiche4684 2 года назад

wow!! so useful!!

@janvi4589 4 месяца назад

top content🔥🔥

@martineneillagateka5779 2 года назад

May God bless you

@AspirantsForum Год назад

Really love this marvellous content and teaching skills. You're lectures are like a beacon. Which textbooks do you recommend us to get such contact in print form?

@theinnominatoidk3821 2 года назад

Thank you

@KseniaKlimova 3 года назад

Thank you so much for such a fantastic and at the same time clear explanation! Much deeper than in an ordinary textbook) Which sources did you use?)

@theCrux 3 года назад

I am glad you found the content helpful :) I use relevant published articles and peer-reviewed discussions to assemble this content.

@islemben4845 Год назад

Thank you so much , and please what about sigma factor as a specificity factor

@crabjoon 15 дней назад

a life save fr!!!

@bioboi4438 Год назад

are the elements shaded in purple always there and part of the promoter or only in some certain circumstances?

@MohammedAhmed-pc9ys 2 года назад

Great work Sir. Well prepared, highly organized lectures with an in-depth explanation. Can you continue your genetics series, please?

@theCrux 2 года назад

Thank you for the kind words and support. I do plan to continue the genetics series. Are you looking for a specific subject/topic in the genetics series?

@MohammedAhmed-pc9ys 2 года назад

Thank you Sir 😊. Regarding the suggestion for the genetics lectures, I guess, mutation types and consequences, as a start, will make perfect sense

@theCrux 2 года назад

Sure, I will put your suggestion on my list.

@pratibhasah6698 6 месяцев назад

I hopw u read my comments can u do video on transferrin and its receptor and groES and gro EL.

@lucidscience9554 3 года назад

Thank you for this amazing video!!! I had a doubt here, is base flipping reversed once DNA scrunching proceeds, as in when the bases at -11 and -7 shift upstream of transcription bubble? And is base flipping exclusively observed in the -10 region only?

@theCrux 3 года назад

Great question! The current understanding is that base flipping is dynamic and reversible once the bubble gets too big - so there is a threshold length of RNA (made during scrunching) that triggers sigma domain 2 to disengage with the TATAAT sequence.

@lucidscience9554 3 года назад

@@theCrux Got it!!! Thank you so much!

@harrypal8459 3 года назад

The initiation by RNA polymerases requires the assembly of a large complex.outline the order of events that result in initiation ,and indicate the type of molecular interactions that are involved in transcription in prokaryotic? Pls reply this question answer in brief and pls plz plz sir reply quickly

@sammilton6593 3 года назад

how dna unwinds during elongation in prokaryotes

@theCrux 3 года назад

Hello Sam! For transcription elongation in prokaryotes, please see this video - ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-7kfqeIjIzMY.html

@NabanitaAB 2 года назад

Please make video on Replication

@theCrux 2 года назад

DNA replication will be the next major series of videos. I am currently structuring and organizing the content. I hope to post those videos somewhere around March.

@NabanitaAB 2 года назад

@@theCrux Thanks a lot sir, glad to know that. It'll be very helpful to me just like your previous contents. Your contents are so much interesting and very very informative. I throughly go through all the videos , and those help me a lot. Keep going sir , you're one of the best. 👌🏻 👍🏻

@tanmoydas7095 Год назад

Sir thank you for the detailed explanation. You told that RNA pol directly binds to UPE for rRNA synthesis, and sigma factor does not bind to this particular UPE(-58,-37). So, does RNA pol for rRNA not need sigma factor?

@theCrux Год назад

Hello. I am not sure if I understand your question. In this video we are discussing mRNA synthesis. I don't think this video touches on rRNA. In prokaryotes, UPE is required for typical regulation mechanisms: Example, lac operon: see, ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-ajFLReouZrU.html

@tanmoydas7095 Год назад

@@theCrux Got it sir. I mis-understood. Thank you

@Biomeducated 4 года назад

Made some promo for your channel on my FB page!

@theCrux 4 года назад

Thank you! I will also set up a Facebook page for my channel so that the information availability/update is more seamless :)

@ritumohapatra6960 15 дней назад

Ek min -10 and -35 coding strand par nahi hote?

@anihysenaj3743 3 года назад

great video! my doubt: you say ''when the number of nts is greater than 10 the transition from abortive tx to elongation takes place'' but when is it that this happens and why or is it casual?

@theCrux 3 года назад

Excellent question! 10 nts is sort of a "magic" number (just an average actually and not a strict rule) - when the RNA is synthesized, the exit channel is blocked by the sigma factor, so the enzyme tries to push it out but it fails often. During this failure, the RNA is released, which we call abortive transcript. But after a few tries, the sigma factor is kicked away and the exit channel is cleared out, which allows the RNA to move out and the transition into the elongation occurs. You can find more details in the DNA scrunching part of this video: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-7kfqeIjIzMY.html

@anihysenaj3743 3 года назад

@@theCrux super clear! Thank you very much!