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The key points covered in this video include:
1. Proteins - Biuret Test
2. Lipids - Emulsion Test
Proteins - Biuret Test
The Biuret test can be used to test for proteins by detecting the presence of peptide bonds. The sample to be tested is placed in a clean test tube. Biuret A (sodium hydroxide) is then added to the test tube. A few drops of Biuret B (copper (II) sulphate) is then added and the solution is gently mixed. If protein is present, the colour of the reagent changes from blue to purple.
Lipids - Emulsion Test
The emulsion test can be used to detect the presence of lipids. The sample to be tested is placed in a clean test tube. Ethanol is then added to the test tube and the solution is mixed thoroughly to dissolve any lipid. Water is then added to the test tube and mixed gently. A cloudy white substance indicates that lipids are present in the sample. This cloudy white substance appears as lipids are not soluble in water so they come out of solution and form an emulsion.
Summary
The Biuret test is used to test for proteins by detecting the presence of peptide bonds
Biuret's reagent turns from blue to purple if protein is present
The emulsion test is used to test for the presence of lipids
Lipid solutions form emulsions when water is added as they are unable to dissolve in water
5 авг 2024