Salting in and salting out | How does salting out happen? | what is salting in of proteins? 

This is the best video for explaining the concept of Salting in and Salting out.... Thankyou dada for this....l would like to ask you about the reference where l can get the exact text of this explanation!!!!!

You are a ray of hope for all of us aspirants like me 🇮🇳🇮🇳

Jazak Allah khyra sir

Thank you. You did something my textbook and my lecturer couldn't do.

Short and clear, thank you!

Beautifully explained thank you! I've been struggling with sample preparation for months now - I have VERY expensive triple-labelled samples for NMR so, naturally, sample stability has been a great concern to ensure we can get the most out of each prep. Have you got any rough kind of buffer optimisation "workflow" that you go through when determining protein solubility? I've thus far tried crude hanging drop precipitate screens using a crystallographers microscope, as well as a thermal denaturation assay utilising a hydrophobic residue-binding dye which infers protein unfolding using qPCR (ThermoFluor I believe is the technique name). Have you got any other suggestions? I'm still struggling somewhat with long term stability.

Thank you .. very very usefull

Ammonium sulphate precipitation follows the procedure explained by you. It is the good and simple method instead of ultracentrifugation and other methods. Nicely explained. Continue.

Very useful thank you

Excellent

our teacher's explaining his subject with ur video, you can be proud bro

BLESS U BRO ,SERIOUSLY 🥺😭u saved my ass in 12 hours i have my exam and this made me finally understand 💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜

Such a great refresher a year after my biochemistry courses! The graphics are really helpful, great work!

The video is useful, thank you

I usually have a hard time understanding how some of these processes work but you did an amazing job at explaining the process! I feel like I understand it and could explain how it works to another individual now. Thank you!

Great video! Just have a litte remark: At 1:52 you mark the carboxylate ion which is part of the protein peptide backbone. Instead you should have marked the carboxylate at the amino acid side chain which is exposed to the enviroment.

This was helpful, thank you

you done good

The video is really informative. 👍

What a great video. Thank you very much

Nice job

Great work, thank you for your presentation, it was clear and informative. the use of graphics greatly helps, keep it up! Would love more about protein separation and electric properties.

Wow well explained ♥️

finally it's crystal clear to me...thank you so much!

Thank you for this great video! Could you make a video about pI calculations of polypeptide chains?

Wonderful 💯💯

awesome! thanks

Very good explanation.Thank you very much!!!!!

I like your animations it helps give a clearer pictuce

This video was very helpful! Thank you! Keep up the excellent work!

Thank you for explaining this concept clearly!!!

Thank you! This helped a lot! I really appreciate your videos.

Thank you - very clear and concise explanation!!

Thank you, your explanation was simple and to the point.

Very informative. Quick and easy to understand. Animation make it more clear.

Best explanation for salting in and out. I want to cite this reference Haha. Thank you so much

This makes so much sense! Thank you.

Great video short and very clear!!!!!

very good ...thank you

Thank you for this lecture video. I have never understood this topic before and the mechanism behind it. This video has done it for me.

Great Animations

Thank you for this video!

A really nice explanation. A hug from Brasil!

Thank you so much! Your explanation was very clear!

Thank you YOU'RE THE BEST👍

Helps a lot! Thanks!

FINALLY, I understood this. Thanks so much!

Thank you for this. Was able to understand it better thanks to this video.

Great video, thank you so so much !

Very helpful! Thank you!

full of knowledge

You saved my day within 6 mins🤗 Thank you!

Helps so much! Thank you

Thank you for this video it was explained very well. Got in on the first take!

Finally i understood..thanks 😅

very nice and point wise explination.Thank you a lot.

Concept clearly explained❤

Your are great, thanks

Great video।।

I appreciate your work sir thanks 😊

Best video ever and very easy to understand! i literally understood everything in 6 mins thank you so much! you are an amazing teacher!!

It was very helpful and easy. Thanks

Thank you ! The concept is clear for me now😊

Thank you for the explanation! Very useful

Thanks alot for the video my concepts are Cleared very well.

Tysm, this was really useful and informative for me. I’ve just had a lecture about protein separation at the university and got confused but your animation helped me so much

Thank you so much for this amazing video

Thanks! It was clear and easy to follow.

Great

I was struggling with this concept but you sir explained it very well. Thank you 🙏🏻

Amazing videoooo

Clear and amazing explanation. Keep up the good work!!

It's so informative.. thanks.

Amazing explanation!

Very well explained 😊Thank you.

Less time with clear concept great 😊 thanks for this 👍

So helpful thank you!

Thank you so much for the explanation 🙏. Your wise way to explain alongside the illustration helped me in clarifying some points about the topic.

Thankyou for information

Thank you very much This was very helpful 👍

Thank you so much for making this fantastic video ❤

You are legit making biochemistry easy for me 🙌

THANK YOU FOR THIS!

Thank you!!!! great vid !!

so helpful and so concise, thanks so much!

You make it easier than I thought, thanks a lot dude.

nice job, sir

Wow that was a great explanation. it was very easy to understand. THANK YOU!

Well explained sir

It was awesome...

Thank u so much 🥰

it was an awesome video. you explained so well. thank you soooooo much

Excellent video 😃

Thank you!!! This video was very well explained and helped me understand something even my professor could not help with

Amazing video!!!

Thank you for your such nice and clear cartoon!

Thanks you so much dear , I have finally clear my concept with this video . Thanks to you once again .

Great bro. Could you talk about why Lanthanum.Cl3.H20 (anhydrase) precipitate in room temperature water? (it has low solubility for sure). This question has nothing to do with protein solubility.

it was very informative and very clear. Thank you so much

Thank you that was amazing !!!