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Sample Preparation by Ashing Method for the Analysis of Heavy Metals & Minerals Using AAS 

MicroChem's Experiments
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10 сен 2024

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Комментарии : 127   
@i2eric452
@i2eric452 Год назад
2:03 did u soak the crucible in nitric acid for 12 hours to make sure there's no other component left in there? used deionized water to wash and remove the acid before using? thank you
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Yes. You are correct.
@i2eric452
@i2eric452 Год назад
9:05 why is there a need to dilute the ash with 1N nitric acid? may we use other acid for this? thank you very much!
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Nitric Acid solution is best for heavy metal and mineral analysis
@rimanahasem81
@rimanahasem81 2 месяца назад
I want to see further steps for heavy metal analysis (how to determine quantity of any metal pb/cr/zn)...do you have any video on this??
@julietdeffinacayo8519
@julietdeffinacayo8519 Год назад
I want to analyze some elements in ash salt (filtrate). The ash was already got from burnt plant residue and the ash salt was prepared by leeching water through the ash material. Is it possible to analyze the already prepared ash salt. It is already in solution.
@MicroChemsExperiments
@MicroChemsExperiments Год назад
If the ash salt is prepared correctly (complete ash with no black color) then you can test your sample with this solution
@user-gc4bp7ur4h
@user-gc4bp7ur4h 2 года назад
Hello! I just wanted to ask - what is the purpose of the filtering step? What is being filtered out? Thank you!
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Filtration is done to ensure that any sort of interference will be filtered out, if any, present in the Ash. Ash particles, which are not dissolved in the diluent, may be filtered out.
@glasslinesmadhes
@glasslinesmadhes 10 месяцев назад
Very much practical oriented. Nice 🎉
@abench975
@abench975 Год назад
can i use this method to determine Calcium, Iron, Copper, Manganese, Zinc, Sodium and Potassium in onion?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Yes you can
@anjalinepal2849
@anjalinepal2849 9 месяцев назад
Which method dry ashing or wet digestion is suitable for determining minerals in liquid supplements???
@SVTenpoche
@SVTenpoche 2 года назад
Can we used this method for the determination of heavy metals in water (waste, naturel) ? if yes, wath is the volume used for sampling? Thank you
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Yes. You can. You can use 2ml of sample
@hamzatyunus2722
@hamzatyunus2722 10 месяцев назад
Why is it necessary to grind the sample into powdery form before ashing?
@deepankitadas8892
@deepankitadas8892 Год назад
Can I use this method to detect heavy metals from cosmetics products like eyeshadow...or should I use wet digestion method
@augustafrancis254
@augustafrancis254 3 года назад
In determining heavy metal metals in tea samples , can this method be used ?
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Yes. You can use.
@syahirahatiqah9459
@syahirahatiqah9459 3 года назад
Hai want to ask, is it necessary to use filter paper Whatman 42 ? because in my lab just have qualitative filter paper such as Ross 1a, Whatman 1PS, Munktell 1F and MGC..
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Yes you can use any of these of smaller pore size among your mentioned filter papers.
@avishakazari6925
@avishakazari6925 3 года назад
Can this method be used for determining heavy metals in plants? from leaves, roots, and stems?
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Yes. You can use
@meenaselvaraj3241
@meenaselvaraj3241 2 года назад
can i use UV-visible spectrophotometer instead of AAS
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
If you have method for UV then you can prepare your sample by this method.
@norhaimadibaratun8350
@norhaimadibaratun8350 Год назад
So satisfying to watch.
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Thank you
@Chemtoliify
@Chemtoliify 2 года назад
Can this method be used for determining Lead (Pb) in lead sulfide ores and lead contentrates.
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Yes.
@Chemtoliify
@Chemtoliify 2 года назад
@@MicroChemsExperiments thank you so much, please keep going
@shathanclay1997
@shathanclay1997 3 года назад
why do you use a pen marker? is required special marker stell pin, the pin that uses is no effect disappear in a muffle furnace?
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
You are absolutely right. Stell pin was not available to us at that moment, thats why we have shown by pen marker.
@mathagroups2865
@mathagroups2865 3 года назад
Kindly mention the maximum temperature and hour. Thank you for your reply
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Max temperature is 650 degree celcius and time is 7 hours.
@tania4681
@tania4681 Год назад
Can I store the prepared sample in refrigerator and at what temperature? I want to send the sample to another place because my laboratory don't have AAS. Thank you
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Store at 4-8 degree Celsius and maintain that temperature during transportation
@nurulainnajwasuhaimai4418
@nurulainnajwasuhaimai4418 Год назад
I plan to measure ash content and then measure mineral content and also heavy metals. For heavy metals i would like to use wet digestion method. In your opinion, do i need to make a wet digestion if i already drying ash from measuring ash content before? Thanks in advance!
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Yes. For heavy metal analysis, wet digestion is better method than the dry ashing.
@nurulainnajwasuhaimai4418
@nurulainnajwasuhaimai4418 Год назад
@@MicroChemsExperiments okay thank youu btw your videos really helps me 👍🏻
@silmeesingkala5902
@silmeesingkala5902 Год назад
If I do dry ashing before and use AAS next day, can I do like that? And what should I do? Because i don't have time enough for it. Thanks for answering
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Keep the Ash sample closed to avoid contamination and prepare the sample from ash on the day of analysis in AAS. Or you can keep and store the prepared solution at 4-8 degree Celsius.
@syahirahatiqah9459
@syahirahatiqah9459 3 года назад
what is the volume of volumetric flask that you use in filtration sample process ? Is it 50ml ?
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
You are right. 50ml
@syahirahatiqah9459
@syahirahatiqah9459 3 года назад
thank you for the reply :)
@foodotechos7600
@foodotechos7600 Год назад
can we use this method for calcium, phosphorous, mg, mn, iodine, sodium and iron , if yes can we this method by using HCL
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Yes
@foodotechos7600
@foodotechos7600 Год назад
@@MicroChemsExperiments and with hcl also?
@ovaneschakoyan7861
@ovaneschakoyan7861 Год назад
Can i use that method for cerels-wheat and maize,and sunflower meal.And any huge loss of mercury with this or not?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
You can use this method
@user-ry4vj3zd3y
@user-ry4vj3zd3y 11 месяцев назад
What's the different between this Ash method and Total Digestion method?
@MicroChemsExperiments
@MicroChemsExperiments 11 месяцев назад
No idea about total digestion method
@teddyku6159
@teddyku6159 2 года назад
Hi. May i know this method suitable for Calcium and Phosphorus? Or wet digestion will ne better.??
@teddyku6159
@teddyku6159 2 года назад
Be*
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Wet Digestion
@aesthetic_shobiez_life
@aesthetic_shobiez_life 5 месяцев назад
How i use liquid solution and how it filtered
@MicroChemsExperiments
@MicroChemsExperiments 5 месяцев назад
Your question is not cleared
@leewilliam3417
@leewilliam3417 6 месяцев назад
Great work😊
@MicroChemsExperiments
@MicroChemsExperiments 6 месяцев назад
Thank you
@shafadaraillona7850
@shafadaraillona7850 3 года назад
in 7:05, if the color still black, does it mean the sample still contain organic matter? is that why we should add some acid?
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
You are right. Presence of Black color means that the sample still contains organic matters. At this point we add some acid. This addition of acid facilitates to break down the Carbon strong bonds making free carbon which can easily be removed from the sample by applying additional heat.
@shafadaraillona7850
@shafadaraillona7850 3 года назад
@@MicroChemsExperiments thank u so much!!
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
@@shafadaraillona7850 most welcome
@tania4681
@tania4681 Год назад
Can I replace the deionized water with distilled water (aquades)? Thank you
@MicroChemsExperiments
@MicroChemsExperiments Год назад
No
@mathagroups2865
@mathagroups2865 3 года назад
Do I use microwave oven for sample dry? It's possible?
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Yes. You can use Microwave Oven for drying the sample.
@yennypaolahernandezherrera7666
hi, can i use this method for determination of arsenic in rice?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Thank to you too
@elaftahir4851
@elaftahir4851 Год назад
Can this method used for liquid sample in cosmetic sample for example??
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Yes. You can
@user-et6pu3gy8u
@user-et6pu3gy8u Год назад
Can we used for PVC VN-65L 1 and PP materials?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
Not sure about it
@agastinrajt1964
@agastinrajt1964 3 года назад
I want to prepare a sample from mango because I need details of the ash content so please give me suggestions about how to prepare a dry sample from mango ie. From fruit.
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
1. Remove moisture by following this video: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-DtSH8n1jbyM.html You will get a dry sample. 2. Determine ash content by following this video: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-n2Qwb8Pw8YE.html
@eyacreates5674
@eyacreates5674 3 года назад
can this method be use to determine the ammount of heavy metal in shrimp
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Yes, you can use
@turkcearapca4297
@turkcearapca4297 2 года назад
Can we use ashing to determine pb ,cu, cd, cromium& mercury in yogurt?
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Yes. You can use this method.
@turkcearapca4297
@turkcearapca4297 2 года назад
Ok, Thanks for your answer❤❤
@swafnams644
@swafnams644 2 года назад
Sir , what is the need of blank sample in there? please explain me
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Blank is needed to minimise the reading of reagent used for this preparation
@Shashikumar55822
@Shashikumar55822 2 года назад
Can this method be used for determining micronutrients in seeds? Plz reply
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Only for heavy metal and mineral determination
@Shashikumar55822
@Shashikumar55822 2 года назад
@@MicroChemsExperiments kindly, tell the process used for the micronutrient analysis in seed? Plz reply
@jestonilanggido2904
@jestonilanggido2904 Год назад
Can this method be used for determining heavy metals in blood serum?
@MicroChemsExperiments
@MicroChemsExperiments Год назад
No. use the following method: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-vAEyeroMfWk.html
@lakykhatun7119
@lakykhatun7119 2 года назад
Can we use the crucible without cover or lid
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
You can but you shouldn't as it may lead to the cross contamination
@mohammadshahdathossain5209
@mohammadshahdathossain5209 3 года назад
Magnificent
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
@Mohammad Shahdat Hossain Thank you sir😊😊
@drabhijeetpachpor5498
@drabhijeetpachpor5498 Год назад
Don't waste water, you can close the tap while rubbing the crucible pot.
@MicroChemsExperiments
@MicroChemsExperiments Год назад
You are correct.
@drabhijeetpachpor5498
@drabhijeetpachpor5498 Год назад
Thank you for your response, your videos are detailed and informative. Keep posting
@MicroChemsExperiments
@MicroChemsExperiments Год назад
@@drabhijeetpachpor5498 Sure. Stay with us
@Shashikumar55822
@Shashikumar55822 2 года назад
Kindly tell the process used to determine the micronutrient content in seeds. Plzz
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-vAEyeroMfWk.html
@Titi-je3ff
@Titi-je3ff 3 года назад
Dnt need to heat with hno3
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Kathuri Kk, No, Ashing Method dont need any heat with HNO3
@mariahameed2183
@mariahameed2183 2 года назад
Can you please share the reference of this method .... Thanking in anticipation
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Combined from AOAC method and journals and validated in-house.
@tahabazine3397
@tahabazine3397 3 года назад
Which method did you follow ?
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Taha bazine, we followed ASEAN Manual For Food Analysis
@tahabazine3397
@tahabazine3397 3 года назад
@@MicroChemsExperiments Thnx 😁
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
@@tahabazine3397 You are most welcome
@karntokaali9778
@karntokaali9778 3 года назад
Please 🥺 I want information a bout prepar blood serum to assess zinc and selenium by GF AAS
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Karntoka Ali, you can analysis your serum sample for Zinc & Selenium by following our video of WET DIGESTION. No modification is needed
@mathagroups2865
@mathagroups2865 3 года назад
I am asking normal microwave oven ( in home)
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
What did you mean by drying? You must need muffled furnace to make ash.
@contehspecial8824
@contehspecial8824 2 года назад
Can you please share the CRM with me Thanks
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
How can I share?
@thesupermysteries1355
@thesupermysteries1355 3 года назад
what is the sample?
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Animal Feed sample
@thesupermysteries1355
@thesupermysteries1355 3 года назад
@@MicroChemsExperiments ok 😉😉
@jones547
@jones547 3 года назад
Can this method be used for the determination of heavy metals in insects?
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Yes. You can use
@jones547
@jones547 2 года назад
@@MicroChemsExperiments what about the wet digestion method? Can we use it for insects heavy metal determination?
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
@@jones547 Yes. You can also use wet digestion method for insects
@jones547
@jones547 2 года назад
@@MicroChemsExperiments Thank you so much 🙏 your video is really helpful for my project 😊
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
@@jones547 Thank you
@purnimapatel3483
@purnimapatel3483 2 года назад
Please tell filter paper no.
@MicroChemsExperiments
@MicroChemsExperiments 2 года назад
Whatman Filter Paper-1
@nitinyadav5004
@nitinyadav5004 3 года назад
Aapki lab Kha h, Mugha seed oil ki iodine value, and some other properties nikalwani h
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
Kalkata main hamara lab hain. Iodine value determination method is already uploaded here. Same mathod should be followed for Mugha seed oil. Thank you.
@nitinyadav5004
@nitinyadav5004 3 года назад
Protein content in oil bhi daal do
@MicroChemsExperiments
@MicroChemsExperiments 3 года назад
@@nitinyadav5004 Detetmonation of ptotein is already uploaded. Pkease follow that method
@nitinyadav5004
@nitinyadav5004 3 года назад
I want to work in your lab
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